Potential of Cellulase of Chaetomium Globosum for Preparation and Characterization of Microcrystalline Cellulose From Water Hyacinth (Eichhornia Crassipes)

Abstract

Objective: This study aimed to increase the yield of microcrystalline cellulose (MCC) made from water hyacinth ɑ-cellulose by enzymatic hydrolysis by using purified enzyme and to find it’s characteristics compared to the reference.
 Methods: In this research, MCC was prepared from water hyacinth powder through the chemical isolation process of ɑ-cellulose, followed by enzymatic hydrolysis with purified cellulase from Chaetomium globosum. The yield of MCC was improved by using purified enzyme and optimization of temperature, pH, and hydrolysis time. Identification was carried out by using ZnCl and infrared spectrophotometry, followed by characterization of MCC include particle size analysis (PSA) and diffracto­gram pattern (X-Ray Diffraction) compared to reference Avicel PH 101.
 Results: Purified enzyme from Chaetomium globosum has high activity with a clear zone area of 45 mm with cellulolytic index 6.5 that almost same as Trichoderma reesei (50 mm), with the cellulase enzyme activity of 6.691 U/ml. The optimum condition was at a temperature of 50⁰C and pH 6.0 with the hydrolysis time of 2 h, which produced 95% yield of MCC. Identification with ZnCl and FTIR spectrum showed positive results, similar to the reference. The results of organoleptic test, particle size analysis, and diffracto­gram pattern (X-Ray Diffraction) showed crystalline characteristic similar to reference (Avicel PH 101).
 Conclusion: Enzyme from Chaetomium globosum has a higher activity of cellulase than Trichoderma reesei with MCC obtained was 95%. Based on the comparison of the organoleptic test, particle size analysis, and diffracto­gram pattern, MCC from water hyacinth has a great potential which showed similar characteristic to reference (Avicel pH 101).

Molecular Characterization and Cellulolytic Capacity of Chaetomium globosum Isolates

“…On the other hand, unstained areas indicated the regions where the CMC have broken down to β-1. Suryadi, et al [29] found that Chaetomium globosum has a high ability of cellulase production (by forming clear zone around the filtrate, 45 mm). Longoni, et al [30] reported the functional role of two cellulases of C. globosum involved in preliminary degradation of plant cell wall polymers and also pointed to the functional role of other enzymes involved in cellulose degradation.…”

Scientific Fungal Report of Moldy Jeans in Indonesia

“…However, limited report found regarding the potential use of this fungi in Indonesia. The only available data was provided by Suryadi et al (2019), in the topic of potential of cellulase of Ch. Globosum for preparation and characterization of microcrystalline cellulose from water hyacinth (Eichhornia crassipes).…”

Isolation of Cellulase from Selected Fungal Strains and Its Use for Manufacture Microcrystal Cellulose from Kapuk Cortex (Ceiba Pentandra (L.) Gaertn)

“…The combined solution was agitated for one hour over a shaking incubator at 160 rpm, 50°C, and then centrifuged for 20 min at 10,000 rpm. The precipitated residue was washed with distilled water (Suryadi et al, 2019) .…”