Potential of bacteria consortium as growth controller of pathogenic fungi Fusarium oxysporum F. sp. cubense (Foc)

Abstract

Utilization of rhizosphere bacteria as agents for controlling of soilborne pathogenic soil microbiomes has been reported in many studies since decades. The aim of the research is identification and characterization the potential of isolated bacteria consortium for controlling of Fusarium oxysporum f.sp. cubense (Foc). The bacteria were selected through the antagonistic test, pathogenic test, and compatibility test. The effectiveness of inhibition of selected bacteria were tested in-vitro through the greenhouse-scale of suppressive soil experiments. The result showed that the Bacillus cereus strain CCM 2010 and BS 3–4B have the highest percentage of inhibition to the Foc (25.68–29.02%). Non-pathogenic bacteria with a percentage of inhibition above 20%, consists of 3 consortia by compatibility testing. Three bacterial consortiums were obtained, the first consortium (BS 3–4B, Bacillus cereus strain CCM 2010, Staphylococcus arlettae strain ATCC 43957, Bacillus cytotoxicus strain NVH 391–98 and Bacillus pseudomycoides strain NBRC 101232), 2nd consortium (Bacillus cereus strain CCM 2010 and Lysinibacillus xylanilyticus strain XDB9), and 3rd consortium (Lysinibacillus xylanilyticus strain XDB9 dan Bacillus pseudomycoides strain NBRC 101232). The data showed application of selected single bacteria able to delay fusarium disease in banana plants more than 45 days post infection in comparison to control plants.

Expression of nutrient uptake-related genes in response to beneficial bacterial consortium application on Musa acuminata

“…The solvent used was distilled water. The media was cooked and sterilized using an autoclave at 15 atm and 121°C for 15 minutes [6].…”

“…The supernatant was discarded, and the pellet was resuspended using sterile 0.9% NaCl until it reached the limit of 2 ml tubes. The bacterial consortium suspension was then homogenized using a vortex mixer, and then the bacterial density was measured using a spectrophotometer [6]. The bacterial density applied based on McFarland 0.5 standard is 10⁸ CFU/ml [7].…”

“…The soil was stirred manually using a shovel evenly. The soil with the bacterial suspension was then stored closed in the greenhouse and left for 1 week [6]. Preparation of suspensions and application of bacterial consortia were carried out four times, starting from the planting period H+0, H+7, H+14, and H+21 on the treatment plants [8].…”

“…bacteria have their efficiency level to provide phosphorus nutrients to plants. It was reported that the bacterial consortium Bacillus pseudomycoides strain NBRC 101232 and Lysinibacillus xylanilyticus strain XDB9 were able to inhibit wilt disease of Fusarium-infected plantain plants on a greenhouse scale so that the bacterial consortium has the potential to become a biocontrol agent [6]. Therefore, this study will focus on analyzing the increase in expression of genes related to nutrient use efficiency after the application of soil bacterial consortium Lysinibacillus xylanilyticus and Bacillus pseudomycoides and prove the potential of soil bacterial consortium Lysinibacillus xylanilyticus and Bacillus pseudomycoides as a biofertilizer candidate.…”

Kemampuan Antagonis Bakteri Endofit dan Konsorsiumnya terhadap Pertumbuhan Jamur Culvularia oryzae Bugnic

“…Konsorsium bakteri dapat menghasilkan enzim kitinase yang mendegradasi dinding sel jamur pathogen, melindungi tanaman terhadap patogen, meningkatkan ketahanan tanaman dan menghasilkan anti-jamur (Hadi et al, 2021)…”