Potential of an endophytic bacteria Bacillus amyloliquefaciens 3–5 as biocontrol agent against potato scab

Cui, Lingxiao; Yang, Chengde; Wang, Yinyu; Ma, Ting; Cai, Fengfeng; Wei, Lijuan; Jin, Mengjun; Osei, Richard; Zhang, Junlian; Tang, Minghua

doi:10.1016/j.micpath.2021.105382

Cited by 26 publications

(11 citation statements)

References 58 publications

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“…Biological control has been recognized as a promising and sustainable measure because of less pollution to the environment and a lower health danger compared with the chemical control. B. amyloliquefaciens with a higher antagonistic activity against phytopathogens can be successfully used as a potential biocontrol agent to control diverse crop diseases, including potato scab caused by Streptomyces griseoplanus [ 24 ]; wheat root rot caused by Bipolaris sorokiniana [ 25 ]; ginseng gray mold caused by B. cinerea [ 26 ]; alfalfa anthracnose caused by C. truncatum [ 27 ]; tomato bacterial wilt caused by Ralstonia solanacearum [ 28 ]; tomato bacterial canker caused by Clavibacter michiganensis [ 29 ]; wheat Fusarium head blight caused by F. graminearum [ 30 ]; and sugar beet leaf spot disease caused by Pseudomonas syringae pv. aptata [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Biological Control of Verticillium Wilt and Growth Promotion in Tomato by Rhizospheric Soil-Derived Bacillus amyloliquefaciens Oj-2.16

et al. 2022

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Verticillium wilt disease caused by Verticillium dahliae seriously affects tomato quality and yield. In this work, strain Oj-2.16 was isolated from rhizosphere soil of the medicinal plant Ophiopogon japonicas and identified as Bacillus amyloliquefaciens on the basis of morphological, physiological, and biochemical characteristics and 16S rDNA sequencing. Strain Oj-2.16 exhibited a high inhibition rate against V. dahliae, and the hyphae inhibited by Oj-2.16 were found to be destroyed on scanning electron microscopy. Lipopeptide and dipeptide genes were detected in the Oj-2.16 genome by PCR amplification involved in surfactin, iturin, fengycin, and bacilysin biosynthesis. In pot experiments, the biocontrol efficacy of strain Oj-2.16 against Verticillium wilt in tomato was 89.26%, which was slightly higher than the efficacy of the chemical fungicide carbendazim. Strain Oj-2.16 can produce indole acetic acid, siderophores, assimilate various carbon sources, and significantly promoted the growth of tomato seedlings by increasing plant height, root length, stem width, fresh weight, and dry weight by 44.44%, 122.22%, 80.19%, 57.65%, 64.00%, respectively. Furthermore, defense-related antioxidant CAT, SOD, POD, and PAL enzyme activities significantly increased and MDA contents significantly decreased in tomato seedlings treated with strain Oj-2.16 upon inoculation of V. dahliae compared with the pathogen-inoculated control. In summary, we concluded that B. amyloliquefaciens Oj-2.16 could be used as a promising candidate for the biocontrol of Verticillium wilt and as plant growth stimulator of tomato.

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Section: Discussionmentioning

confidence: 99%

Biological Control of Verticillium Wilt and Growth Promotion in Tomato by Rhizospheric Soil-Derived Bacillus amyloliquefaciens Oj-2.16

et al. 2022

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“…amyloliquefaciens to show antibacterial activity against Streptomyces griseoplanus which causes potato scabs. Six diverse antibacterial compounds were detected after PCR analysis [ 69 ]. Iturin and surfactin-like compounds were reported from endophytic B .…”

Section: Discussionmentioning

confidence: 99%

Endophytic bacteria Klebsiella spp. and Bacillus spp. from Alternanthera philoxeroides in Madiwala Lake exhibit additive plant growth-promoting and biocontrol activities

Biswas,

Philip,

Jayaram

et al. 2023

Journal of Genetic Engineering and Biotechnology

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Background The worldwide increase in human population and environmental damage has put immense pressure on the overall global crop production making it inadequate to feed the entire population. Therefore, the need for sustainable and environment-friendly practices to enhance agricultural productivity is a pressing priority. Endophytic bacteria with plant growth-promoting ability and biocontrol activity can strongly enhance plant growth under changing environmental biotic and abiotic conditions. Herein, we isolated halotolerant endophytic bacteria from an aquatic plant, Alternanthera philoxeroides, from the polluted waters of Madiwala Lake in Bangalore and studied their plant growth promotion (PGP) and biocontrol ability for use as bioinoculant. Results The isolated bacterial endophytes were screened for salt tolerance ranging from 5 to 15% NaCl concentration. Klebsiella pneumoniae showed halotolerant up to 10% NaCl and Bacillus amyloliquefaciens and Bacillus subtilis showed up to 15%. All three strains demonstrated good PGP abilities such as aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, phosphate solubilization, ammonia production, and nitrogen fixation. In addition, K. pneumoniae also exhibited high indoleacetic acid (IAA) production (195.66 ± 2.51 µg/ml) and potassium solubilization (2.13 ± 0.07 ppm). B. amyloliquefaciens and B. subtilis showed good extracellular enzyme production against cellulase, lipase, protease, and amylase. Both the isolates showed a broad spectrum of antimicrobial activity against the tested organisms. The optimization of IAA production by K. pneumoniae was done by the response surface methodology (RSM) tool. Characterization of IAA produced by the isolate was done by gas chromatography-mass spectrometry (GCMS) analysis. The enhanced plant growth-promoting ability of K. pneumoniae was also demonstrated using various growth parameters in a pot trial experiment using the seeds of Vigna unguiculata. Conclusion The isolated bacterial endophytes reported in this study can be utilized as PGP promotion and biocontrol agents in agricultural applications, to enhance crop yield under salinity stress. The isolate K. pneumoniae may be used as a biofertilizer in sustainable agriculture and more work can be done to optimize the best formulations for its application as a microbial inoculant for crops.

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“…They found that low concentration of CuONPs increased the production of actinorhodin (ACT) antibiotics, whereas high concentrations inhibited this process ( Liu et al, 2019 ). Common scab disease occurs throughout the world's potato-cultivating regions as an accusative agent of Streptomyces scabies and other strains, including S. acidiscabies, S. turgidiscabies, S. ipomoeae, and S. griseoplanus ( Loria et al, 2006 , Lambert et al, 2007 , Cui et al, 2021 ). Numerous potato crops contain Bacillus , and they are known for their potential as biocontrol agents ( Han et al, 2005 , Khedher et al, 2015 , Cui et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Common scab disease occurs throughout the world's potato-cultivating regions as an accusative agent of Streptomyces scabies and other strains, including S. acidiscabies, S. turgidiscabies, S. ipomoeae, and S. griseoplanus ( Loria et al, 2006 , Lambert et al, 2007 , Cui et al, 2021 ). Numerous potato crops contain Bacillus , and they are known for their potential as biocontrol agents ( Han et al, 2005 , Khedher et al, 2015 , Cui et al, 2021 ). B. megaterium L2 has yielded five bioactive compounds with antimicrobial activity: phenylacetic acid, behenic acid, erucamide, palmitic acid, and β-sitosterol ( Xie et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Draft genome sequence of potato crop bacterial isolates and nanoparticles-intervention for the induction of secondary metabolites biosynthesis

Al-Theyab¹,

Alrasheed²,

Abuelizz³

et al. 2023

Saudi Pharmaceutical Journal

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Potential of an endophytic bacteria Bacillus amyloliquefaciens 3–5 as biocontrol agent against potato scab

Cited by 26 publications

References 58 publications

Biological Control of Verticillium Wilt and Growth Promotion in Tomato by Rhizospheric Soil-Derived Bacillus amyloliquefaciens Oj-2.16

Biological Control of Verticillium Wilt and Growth Promotion in Tomato by Rhizospheric Soil-Derived Bacillus amyloliquefaciens Oj-2.16

Endophytic bacteria Klebsiella spp. and Bacillus spp. from Alternanthera philoxeroides in Madiwala Lake exhibit additive plant growth-promoting and biocontrol activities

Draft genome sequence of potato crop bacterial isolates and nanoparticles-intervention for the induction of secondary metabolites biosynthesis

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