Draft genome sequence of potato crop bacterial isolates and nanoparticles-intervention for the induction of secondary metabolites biosynthesis

Al-Theyab, Nada; Alrasheed, Omar; Abuelizz, Hatem A.; Liang, Mingtao

doi:10.1016/j.jsps.2023.04.016

Cited by 2 publications

(6 citation statements)

References 60 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HPLC profiles of compounds 1 and 2 have previously been studied [ 22 ]. However, they were not separated well as the peaks were slightly overlapped.…”

Section: Resultsmentioning

confidence: 99%

“…The AntiSMASH prediction has previously been discussed and suggested the naringenin molecule that appeared in the MIBiG comparison of the molecules with structural similarities correlated with those found in S. calvuligerus [ 22 ]. Indeed, the HPLC profile of P. megaterium from crude extract isolations showed daidzein (1) and genistein (2) isoflavones as shown in Section 3.2 on structure elucidation.…”

Section: Resultsmentioning

confidence: 99%

“…Kamchatica CHI gene, as shown in Figure 3 . P. megaterium’s genome draft has previously been studied and identified using nanopore technology [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

“…The extraction of targeted compounds after AuNP intervention in P. megaterium metabolism were reported previously [ 22 ]. Briefly, the biomass of bacterial growth was centrifuged, and the cell-free supernatants were obtained and then extracted with an equal volume of ethyl acetate.…”

Section: Methodsmentioning

confidence: 99%

“…Kamchatica CHI gene, as shown in Figure 3. P. megaterium's genome draft has previously been studied and identified using nanopore technology [22]. Then, we were more interested in finding similar genes from the chalcone synthase/stilbene synthase (CHS/STS) family in other species, such as S. griseus, S. calvuligerus, and Saccharopolyspora erythraea.…”

Section: Biosynthetic Pathway For the Two Isolated Compoundsmentioning

confidence: 99%

See 4 more Smart Citations

Priestia megaterium Metabolism: Isolation, Identification of Naringenin Analogues and Genes Elevated Associated with Nanoparticle Intervention

Al-Theyab,

Abuelizz,

Al-Hamoud

et al. 2023

CIMB

Self Cite

View full text Add to dashboard Cite

The impact of gold nanoparticles (AuNPs) on the biosynthetic manipulation of Priestia megaterium metabolism where an existing gene cluster is enhanced to produce and enrich bioactive secondary metabolites has been studied previously. In this research, we aimed to isolate and elucidate the structure of metabolites of compounds 1 and 2 which have been analyzed previously in P. megaterium crude extract. This was achieved through a PREP-ODS C18 column with an HPLC-UV/visible detector. Then, the compounds were subjected to nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS), and Fourier-transform infrared spectroscopy (FT-IR) techniques. Furthermore, bioinformatics and transcriptome analysis were used to examine the gene expression for which the secondary metabolites produced in the presence of AuNPs showed significant enhancement in transcriptomic responses. The metabolites of compounds 1 and 2 were identified as daidzein and genistein, respectively. The real-time polymerase chain reaction (RT-PCR) technique was used to assess the expression of three genes (csoR, CHS, and yjiB) from a panel of selected genes known to be involved in the biosynthesis of the identified secondary metabolites. The expression levels of two genes (csoR and yijB) increased in response to AuNP intervention, whereas CHS was unaffected.

show abstract

“…The HPLC profiles of compounds 1 and 2 have previously been studied [ 22 ]. However, they were not separated well as the peaks were slightly overlapped.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Kamchatica CHI gene, as shown in Figure 3 . P. megaterium’s genome draft has previously been studied and identified using nanopore technology [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Biosynthetic Pathway For the Two Isolated Compoundsmentioning

confidence: 99%

See 3 more Smart Citations