Potential Inhibition by Phaleria macrocarpa Leaves Ethanol Extract on Ki-67 Expression in Distal Colon Mouse

Kusmardi, Kusmardi; Wiyarta, Elvan; Estuningtyas, Ari; Sahar, Nurhuda; Midoen, Yurnadi Hanafi; Tedjo, Aryo; Pakpahan, Alfred

doi:10.5530/pj.2021.13.56

Cited by 9 publications

(7 citation statements)

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“…The protocol was modi ed from previous papers [11][12][13]. The para n-preserved tissues were sectioned to a thickness of 3 µm using a microtome and mounted on coated transparencies.…”

Section: Slides Preparationmentioning

confidence: 99%

Prognostic Significance of CD133 Expression in Breast Cancer: Correlation with the Nottingham Prognostic Index

rustamadji,

wiyarta

2024

Preprint

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Background CD133 is a transmembrane protein, identified as a cancer stem cell marker, and its prognostic significance in various malignancies, including breast cancer, has been studied. This study aims to investigate the prognostic significance of CD133 expression in breast cancer and its correlation with the Nottingham Prognostic Index (NPI). Methods This retrospective cohort study examined specimens from female patients diagnosed with Invasive Breast Cancer (IBC). CD133 expression was evaluated using the H-score based on staining intensity. Patient data such as age, tumor dimensions, Nottingham Prognostic Index, and other clinicopathological parameters were collected. Comparative univariate analysis and correlation analysis were performed. Results Our study included 60 patients, with significant differences in CD133 H-score values were observed among lymphovascular invasion (LVI) patients (p < 0.001), patients with metastasis (p < 0.001), and patients with NPI (p = 0.005). There was a significant positive correlation between CD133 H-score and NPI (r: 0.31; p0.014) and also between NPI and tumor size (r: 0.66; p0.001). Conclusion Our findings suggest that CD133 may serve as a prognostic marker in breast cancer, with higher expression levels linked to more aggressive disease characteristics and a poorer prognosis as indicated by NPI.

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“…The protocol was modi ed from previous papers [11][12][13]. The para n-preserved tissues were sectioned to a thickness of 3 µm using a microtome and mounted on coated transparencies.…”

Section: Slides Preparationmentioning

confidence: 99%

Prognostic Significance of CD133 Expression in Breast Cancer: Correlation with the Nottingham Prognostic Index

rustamadji,

wiyarta

2024

Preprint

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“…A study performed by Kusmardi and the team proved the ability of P. macrocarpa leaf ethanol extract (PMLEE) to upregulate the expression of Caspase-3 ( Kusmardi et al, 2021a ). It decreased the expression of Ki-67 ( Kusmardi et al, 2021b ) in induced dextran sodium sulfate (DSS) colon cells of Balb/c mice. Caspase-3 was used as an apoptosis marker, while Ki-67 was used as a proliferative marker in the ulcerative colitis study.…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Phaleria macrocarpa (Scheff.) Boerl.: An updated review of pharmacological effects, toxicity studies, and separation techniques

Ahmad¹,

Mazlan²,

Aziz³

et al. 2023

Saudi Pharmaceutical Journal

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“…The staining technique is followed by Primariadewi et al (2021) and [3], [4], [13], [14]. According to standard protocols, a paraffin slice of a breast cancer specimen was deparaffinized in xylol (Merck, Jakarta, Indonesia) and rehydrated for 5 min in a 96%, 70%, and distilled water series (Merck, Jakarta, Indonesia).…”

Section: Slide Preparation and Ihc Stainingmentioning

confidence: 99%

Exploring the Expression of Survivin on Neoadjuvant Chemotherapy in Invasive Breast Carcinoma

Rustamadji

Wiyarta

Anggreani

2022

Open Access Maced J Med Sci

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Background: Biomarkers are required to monitor the response to neoadjuvant chemotherapy (NC) in patients with invasive breast cancer (IBC). The purpose of this study is to determine the function of Survivin in the administration of NC, both taxane- and non-taxane-based, to patients with IBC. Methods: Thirty-one samples were categorized according to the NC's administrative status (before or after) and the type of NC used (taxane- or non-taxane-based). Age, tumor grade, receptor status (ER, PR, HER2, Ki-67), and survivin expression were evaluated. Survivin expressions were evaluated by IHC staining and categorized according median H-score cut-offs, while other data were collected from archives. Data was gathered and analyzed using generalized linear model. Results: Survivin expression decreased following NC administration, although not significantly (p=0.285). The taxane group had lower survivin expression. Statistically, this was not significant (p=0.329). The non-taxane group had the same outcome (p=0.792). The decline in survivin expression was greater in the taxane group than in the non-taxane group, although it was not statistically significant (p=0.369). Conclusion: Although the changes in survivin expression were not statistically significant, when clinical and laboratory data are analyzed, survivin expression has the potential to be a predictive biomarker of NC response as well as clinical outcome in IBC.

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Potential Inhibition by Phaleria macrocarpa Leaves Ethanol Extract on Ki-67 Expression in Distal Colon Mouse

Cited by 9 publications

References 0 publications

Prognostic Significance of CD133 Expression in Breast Cancer: Correlation with the Nottingham Prognostic Index

Prognostic Significance of CD133 Expression in Breast Cancer: Correlation with the Nottingham Prognostic Index

Phaleria macrocarpa (Scheff.) Boerl.: An updated review of pharmacological effects, toxicity studies, and separation techniques

Exploring the Expression of Survivin on Neoadjuvant Chemotherapy in Invasive Breast Carcinoma

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