Exploring the Expression of Survivin on Neoadjuvant Chemotherapy in Invasive Breast Carcinoma

Rustamadji, Primariadewi; Wiyarta, Elvan; Anggreani, Ineke

doi:10.3889/oamjms.2022.9940

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…The protocol was modi ed from previous papers [11][12][13]. The para n-preserved tissues were sectioned to a thickness of 3 µm using a microtome and mounted on coated transparencies.…”

Section: Slides Preparationmentioning

confidence: 99%

Prognostic Significance of CD133 Expression in Breast Cancer: Correlation with the Nottingham Prognostic Index

rustamadji,

wiyarta

2024

Preprint

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Background CD133 is a transmembrane protein, identified as a cancer stem cell marker, and its prognostic significance in various malignancies, including breast cancer, has been studied. This study aims to investigate the prognostic significance of CD133 expression in breast cancer and its correlation with the Nottingham Prognostic Index (NPI). Methods This retrospective cohort study examined specimens from female patients diagnosed with Invasive Breast Cancer (IBC). CD133 expression was evaluated using the H-score based on staining intensity. Patient data such as age, tumor dimensions, Nottingham Prognostic Index, and other clinicopathological parameters were collected. Comparative univariate analysis and correlation analysis were performed. Results Our study included 60 patients, with significant differences in CD133 H-score values were observed among lymphovascular invasion (LVI) patients (p < 0.001), patients with metastasis (p < 0.001), and patients with NPI (p = 0.005). There was a significant positive correlation between CD133 H-score and NPI (r: 0.31; p0.014) and also between NPI and tumor size (r: 0.66; p0.001). Conclusion Our findings suggest that CD133 may serve as a prognostic marker in breast cancer, with higher expression levels linked to more aggressive disease characteristics and a poorer prognosis as indicated by NPI.

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“…The protocol was modi ed from previous papers [11][12][13]. The para n-preserved tissues were sectioned to a thickness of 3 µm using a microtome and mounted on coated transparencies.…”

Section: Slides Preparationmentioning

confidence: 99%

Prognostic Significance of CD133 Expression in Breast Cancer: Correlation with the Nottingham Prognostic Index

rustamadji,

wiyarta

2024

Preprint

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“…The staining technique is followed by Primariadewi et al (2021) and Kusmardi et al (2021) (8,(19)(20)(21)(22). According to typical protocols, a paraffin slice of a breast cancer specimen was deparaffinized in xylol (Merck, Jakarta, Indonesia) and rehydrated in 96%, 70% absolute alcohol series (Merck, Jakarta, Indonesia) and distilled water for 5 minutes.…”

Section: Slide Preparation and Ihc Stainingmentioning

confidence: 99%

Exploring the Integrated Role of AKT2, CD44v6, And MT1-MMP as Predictors of Axillary Lymph Node Metastasis in Invasive Breast Carcinoma of No Special Type

2022

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Scan to discover onlineBackground & Objective: Invasive breast carcinoma of no special type (IBC-NST) is the most common type of breast cancer, which mainly causes axillary lymph-node metastasis (ALNM). Building on our previous research, we wanted to explore the optimal combination of AKT2, CD44v6, and MT1-MMP for the ALNM prediction. Methods:The presence or absence of ALNM was used to separate 46 paraffin blocks containing IBC-NST primary tumors into two groups. Age, tumor grade, tumor size, receptor status (ER, PR, HER2, Ki-67, TOP2A), and test biomarker expression were evaluated. Biomarker expressions were assessed by IHC staining and categorized according to their respective cut-offs from our previous study, while other data were collected from archives. Data was gathered and analyzed using univariate, multivariate, and AUROC models. Results:The expression of CD44v6 (OR: 12.77, 95% CI: 2.18-87.12, P=0.005) was identified as the independent variable for ALNM. Meanwhile, AKT2 expression (OR: 3.22, 95% CI: 0.36-22.41, P=0.237) and MT1-MMP expression (OR: 5.35, 95% CI: 0.83-34.54, P=0.078) did not demonstrate a statistically significant independent association in respect to ALNM. Combining AKT2 and MT1-MMP on CD44v6 increased overall accuracy by 4% compared to CD44v6 alone (AUROC 0.89 vs. 0.85). Conclusion:The combined usage of AKT2, CD44v6, and MT1-MMP revealed no significant change compared to CD44v6 alone. Due to the cost and practicality, we propose using CD44v6 as a predictor biomarker of ALNM in IBC-NST.

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“…Due to of the association of NFkB, Survivin, and Cyclin D1 with NC resistance, the expression level of each of these biomarkers can be different at pre- and post-NC in IBC ( 10 ). Analysis of expression levels before and after the NC is critical because these findings may support the application of these biomarkers as predictive markers of NC response in IBC ( 11 , 12 ).…”

Section: Introductionmentioning

confidence: 99%

Correlation of Before and After Invasive Breast Cancer Neoadjuvant Chemotherapy for NFkB, Cyclin D1, and Survivin Expression

2023

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Background & Objective: Patients undergoing neoadjuvant chemotherapy (NC) for invasive breast cancer (IBC) therapy need biomarkers to track their progress. Because of the relationship between NFkB, Survivin, and Cyclin D1 with NC resistance, the different expression levels of each of these biomarkers can be different between pre- and post-NC in IBC. However, no research has examined the correlation between these biomarkers before and after the NC expression. This study aimed to determine the correlation among them. Methods: Biomarkers expression (low and high) was used to classify 30 samples. ER, PR, HER2, Ki-67 status, tumor grade, age, and NC response were assessed. The amounts of Survivin, Cyclin D1, and NFkB were evaluated using immunohistochemistry, and the samples were classified based on the cut-off. Chi-square and linear regression were used to evaluate the data. Results: No significant association was found with the changes in the expression of Survivin, Cyclin D1, and NFkB, both before and after the NC. Significant moderate correlations were shown between before and after the NC Survivin expression (r = 0.513) and Cyclin D1 expression (r = 0.543). The correlation between expression of NFkB before and after the NC was not significant. Conclusion: The high potential of these proteins as prognostic indicators was demonstrated by the strong positive association between the expression of Survivin and Cyclin D1 before and after the NC. This upregulation of biomarkers indicates chemoresistance in developing IBC in the presence of NC.

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Exploring the Expression of Survivin on Neoadjuvant Chemotherapy in Invasive Breast Carcinoma

Cited by 3 publications

References 25 publications

Prognostic Significance of CD133 Expression in Breast Cancer: Correlation with the Nottingham Prognostic Index

Prognostic Significance of CD133 Expression in Breast Cancer: Correlation with the Nottingham Prognostic Index

Exploring the Integrated Role of AKT2, CD44v6, And MT1-MMP as Predictors of Axillary Lymph Node Metastasis in Invasive Breast Carcinoma of No Special Type

Correlation of Before and After Invasive Breast Cancer Neoadjuvant Chemotherapy for NFkB, Cyclin D1, and Survivin Expression

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