Potential factors involved in the early pathogenesis of Streptococcus uberis mastitis: a review

Fessia, Alumine Soledad; Odierno, Liliana

doi:10.1007/s12223-021-00879-9

Cited by 10 publications

(10 citation statements)

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“…Mastitis is considered a serious problem because it not only causes huge economic losses (through reduced milk yield and quality, early culling and corresponding treatment costs), but also threatens public health [ 1 , 2 ]. Streptococcus uberis ( S. uberis ) is a bacteria classified as a major environmental pathogen associated with subclinical and clinical mastitis in lactating cows, non-lactating cows and heifers [ 3 , 4 ]. Because it is ubiquitous in the cow’s environment (e.g., present in water, feces, barn environment, bedding material, etc.…”

Section: Introductionmentioning

confidence: 99%

Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis

Wang

Bissonnette

Laterrière

et al. 2022

J Animal Sci Biotechnol

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Background Mastitis caused by different pathogens including Streptococcus uberis (S. uberis) is responsible for huge economic losses to the dairy industry. In order to investigate the potential genetic and epigenetic regulatory mechanisms of subclinical mastitis due to S. uberis, the DNA methylome (whole genome DNA methylation sequencing) and transcriptome (RNA sequencing) of milk somatic cells from cows with naturally occurring S. uberis subclinical mastitis and healthy control cows (n = 3/group) were studied. Results Globally, the DNA methylation levels of CpG sites were low in the promoters and first exons but high in inner exons and introns. The DNA methylation levels at the promoter, first exon and first intron regions were negatively correlated with the expression level of genes at a whole-genome-wide scale. In general, DNA methylation level was lower in S. uberis-positive group (SUG) than in the control group (CTG). A total of 174,342 differentially methylated cytosines (DMCs) (FDR < 0.05) were identified between SUG and CTG, including 132,237, 7412 and 34,693 DMCs in the context of CpG, CHG and CHH (H = A or T or C), respectively. Besides, 101,612 methylation haplotype blocks (MHBs) were identified, including 451 MHBs that were significantly different (dMHB) between the two groups. A total of 2130 differentially expressed (DE) genes (1378 with up-regulated and 752 with down-regulated expression) were found in SUG. Integration of methylome and transcriptome data with MethGET program revealed 1623 genes with significant changes in their methylation levels and/or gene expression changes (MetGDE genes, MethGET P-value < 0.001). Functional enrichment of genes harboring ≥ 15 DMCs, DE genes and MetGDE genes suggest significant involvement of DNA methylation changes in the regulation of the host immune response to S. uberis infection, especially cytokine activities. Furthermore, discriminant correlation analysis with DIABLO method identified 26 candidate biomarkers, including 6 DE genes, 15 CpG-DMCs and 5 dMHBs that discriminated between SUG and CTG. Conclusion The integration of methylome and transcriptome of milk somatic cells suggests the possible involvement of DNA methylation changes in the regulation of the host immune response to subclinical mastitis due to S. uberis. The presented genetic and epigenetic biomarkers could contribute to the design of management strategies of subclinical mastitis and breeding for mastitis resistance.

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Section: Introductionmentioning

confidence: 99%

Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis

Wang

Bissonnette

Laterrière

et al. 2022

J Animal Sci Biotechnol

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“…uberis is mainly environmental, but within-cow and cow-to-cow transmission is also possible based on epidemiological studies. Moreover, the pathogen can easily adapt to a variety of niches, including the skin, oral cavity, feces, respiratory tract, and rectum, due to its nutritional flexibility (Fessia and Odierno 2021 ). The establishment of S .…”

Section: Discussionmentioning

confidence: 99%

“…Mastitis-associated risk factors in lactating and non-lactating cows include udder structure, genetic aspects, diet, age of cows, stage of lactation, milking system, milking interval, hygiene, and dry period (Zigo et al 2021 ). The main cause of bovine mastitis is pathogens like Staphylococcus aureus , Escherichia coli , Streptococcus uberis , S. agalactiae , S. dysgalactiae , and Klebsiella pneumoniae (Fessia and Odierno 2021 ). The incidence of mastitis has decreased since the introduction of the mastitis five-point plan in the 1970s.…”

Section: Introductionmentioning

confidence: 99%

“…uberis have been described, including plasminogen activator A (PauA), acid capsule, hyaluronidase, S . uberis adhesion molecule (SUAM), glyceraldehyde-3-phosphate dehydrogenase C (GapC), Opp proteins, elongation factor Ts, hemolysin-like protein, sortase A (SrtA), surface lipoprotein, fibronectin-binding protein, C5a peptidase, lactoferrin-binding protein, and collagen-like surface-anchored protein (Fessia and Odierno 2021 ). Among these factors, PauA, GapC, and SUAM have been extensively studied for the development of vaccines against S .…”

Section: Introductionmentioning

confidence: 99%

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In silico analysis of virulence factors of Streptococcus uberis for a chimeric vaccine design

Yılmaz Çolak

2024

In Silico Pharmacol.

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Streptococcus uberis is one of the causative agents of bovine mastitis, which has detrimental effects on animal health and the dairy industry. Despite decades of research, the requirement for effective vaccines against the disease remains unmet. The goal of this study was to create a multi-epitope vaccine using five virulence factors of S. uberis through the reverse vaccinology approach, which has been employed due to its high efficiency and applicability. Plasminogen activator A (PauA), glyceraldehyde-3-phosphate dehydrogenase C (GapC), C5a peptidase, S. uberis adhesion molecule (SUAM), and sortase A (SrtA) were selected for the T cytotoxic (CTL) and B cell epitope analyses as they were extensively studied in S. uberis or other pathogens. Eighteen CTL and ten B cell epitopes that were antigenic, non-toxic, and non-allergenic were selected in order to design a chimeric vaccine candidate that in silico analysis revealed to be potentially immunogenic, non-allergenic, and stable. Molecular docking analysis of the vaccine candidate with Toll-like receptor (TLR) 2 and TLR 4 revealed stable interactions between the candidate and the immune receptors. Meanwhile, the stability of the docked complexes was confirmed using normal mode analysis. Additionally, in silico immune simulation of the vaccine candidate demonstrated the stimulation of primary immune responses, indicating that the chimeric protein can hold promise as a viable vaccine candidate for preventing S. uberis mastitis. Moreover, the current study can provide a background for designing epitope-based vaccines based on the explored epitopes.

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“…Most of the bacteria grow strictly anaerobically, most of which are saprophytic parasitic bacteria and a few are pathogens. It secretes exotoxins and enzymes that can cause disease in humans and animals [30]. Finally, for Streptococcus uberis, related reports mainly revolve around bovine mastitis, there are few reports in humans [31].…”

Section: Discussionmentioning

confidence: 99%

Characterizing Severe Early Childhood Caries Microbiota through Culturomics

Zhao,

2023

IJCEMR

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Severe early childhood caries (SECC) is a common widespread caries affecting multiple deciduous teeth in children, which seriously affects children's physical and mental health and growth. Investigation of their pathogenic flora is essential. Two children with SECC and two caries-free children of the same age were included in this study. Using cultureomics techniques, that is, microorganisms in oral dental plaque were isolated and cultured by setting different conditions (temperature, oxygen conditions, culture medium). Species were identified by 16S rRNA sequencing, and strain function was analyzed in depth in combination with clinical information. A total of 37 species were identified in this study. 4 species had never been associated with the oral cavity before, and 3 of them had never been isolated from clinical specimens. This pilot study was able to provide some new information on the complexity of the microbiota in SECC patients versus healthy children of the same age.

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Potential factors involved in the early pathogenesis of Streptococcus uberis mastitis: a review

Cited by 10 publications

References 152 publications

Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis

Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis

In silico analysis of virulence factors of Streptococcus uberis for a chimeric vaccine design

Characterizing Severe Early Childhood Caries Microbiota through Culturomics

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