Potential enzyme activity of thermophilic bacteria from hot spring in Egypt

Abstract

This study aims to isolate and identify thermophilic hydrolytic bacteria from hot spring in South Sinai, Egypt for several industrial applications. In this work, 29 bacterial isolates from hot spring in Egypt were isolated and screened for the production of three thermozymes (amylase, cellulase and protease) at different high temperatures. Fifteen isolates were amylase producer, twenty-one were cellulase producer and twelve isolates were protease producer at different high temperatures. Ten bacterial isolates (34.5%) produced the three extracellular enzymes. All bacterial isolates were identified phenotypically as Bacillus spp. This study concludes that hot springs in Egypt is a source for the isolation of thermophilic bacteria producing thermostable enzymes.

Isolation, characterization, and cloning of thermostable pullulanase from Geobacillus stearothermophilus ADM-11

“…The pullulanase gene was reported in mesophilic bacteria such as Bacillus pseudofirmus 703 ( Lu et al, 2018 ) and Bacillus strain KSM-1876 ( Hatada et al, 2001 ), thermophilic bacteria such as Thermotoga maritime MSB8 ( Zhao et al, 2023 ), hyperthermophiles (such as Rhodothermus marinus ( Gomes et al, 2003 ), and Thermococcus siculi HJ21 ( Wu et al, 2023 ). Extremophilic microorganisms, which thrive under severe conditions, can be classified into 7 families; acidophiles, alkaliphilies, halophiles, metalopiles, psychrophlies, and thermophiles ( Taha et al, 2020 ). These thermophiles prefer a temperature of 50 °C or higher for the production of enzymes such as pectinase, cellulose, chitinase, amylase, lipase, DNA polymerases, etc.…”

Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia

“…4 Thermophiles (optimal growth above 50°C) and hyperthermophiles (optimal growth above 80°C) are among the most studied microorganisms in extreme environments. 5,6 Their natural biotopes vary and can be terrestrial (geothermal springs, desert and volcanic soils, oil drills, and compost) or marine (deep hydrothermal mountains). Extreme temperature, pH, and anoxic conditions are responsible for the high genomic and metabolic flexibility of thermophilic microbial communities colonising these hot environments, making the exploitation of such microbial proteins attractive for biotechnological applications.…”

Isolation and characterization of potential multiple extracellular enzyme-producing bacteria from waste dumping area in Addis Ababa

“…Many possible mesophilic microbial strains have been discovered from landfill soil and waste, including bacteria and fungi that can rapidly proliferate and biodegrade organic wastes despite high organic matter concentrations ( Krishnan et al, 2021 ; Song et al, 2018 ). Extremophilic microorganisms are classified according to the type of extreme condition which they prefer to grow into seven families, thermophiles, psychrophiles, halophiles, acidophiles, alkaliphiles, metalophiles, and piezophiles ( Taha et al, 2020 ). Extremophiles are involved in thermophilic microorganisms (those with an optimum growth temperature of 50 °C or higher) because they produced by thermostable enzymes (such as cellulases, amylases, pectinases, chitinases, proteases, lipases, xylanases, and DNA polymerases); all such enzymes have unique features that make them suitable for biotechnological processes at high temperatures ( Mohammad et al, 2017 ).…”