Isolation and characterization of potential multiple extracellular enzyme-producing bacteria from waste dumping area in Addis Ababa

Masi, Chandran; Tebiso, Abel; V, Selva Kumar K

doi:10.1016/j.heliyon.2022.e12645

Cited by 11 publications

(5 citation statements)

References 44 publications

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“…The majority of the strains showed a white colour on the NA. Similar results were reported by Ifandi and Alwi 26 and Masi et al, 28 who reported the dominant white and yellow colours of thermophilic bacteria. The majority had round colonies with an irregular form, elevated elevation, rough surface, and translucent opacity.…”

Section: Morphological and Biochemical Characterization Of The Isolat...supporting

confidence: 91%

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Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia

Saidan,

Jarboui,

Alsharari

et al. 2024

J. Pure Appl. Microbiol.

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This study aims to investigate the morphological and biochemical characteristics of thermophilic bacteria isolated from thermophilic biotopes in the Al-Jouf region of Saudi Arabia for the first time. Additionally, the production of thermostable bacterial enzymes (proteases, amylases, cellulases, gelatinases, and lipases) was investigated. Six main bacterial species were identified via 16S rRNA sequencing and phylogenetic analysis. Twenty water and soil samples were collected from several thermophilic sites in the Dumat Al-Jandal and Al-Qurayyat cities. In total, 28 bacterial strains were isolated and biochemically characterized. Most isolated strains showed high protease and amylase production. 46 and 28% of isolated strains showed the production of cellulase and gelatinase, respectively. However, lipase production was not observed in any isolated bacteria. Four Bacillus licheniformis and two Bacillus subtilis strains exhibited high GC content (55%). Our findings suggest thermophilic bacteria as a source of thermostable enzymes for pharmaceutical and industrial applications.

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Section: Morphological and Biochemical Characterization Of The Isolat...supporting

confidence: 91%

“…The same authors showed that Aneurinibacillus, Aeribacillus, and Anoxybacillus exhibited high enzyme activity. Recently, Masi et al 28 isolated 102 thermophilic strains from the Addis Ababa landfill site (2023). Among the bacteria analysed, 35 were…”

Section: Production Of Extracellular Thermostable Enzymesmentioning

confidence: 99%

Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia

Saidan,

Jarboui,

Alsharari

et al. 2024

J. Pure Appl. Microbiol.

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“…Then, the serial dilution was carried out using physiological NaCl to minimize or reduce the number or density of microbes suspended in the liquid so that their colonies could be well-separated. The method used for bacterial isolation is the spread plate method, which is suitable to provide better visibility on colony shapes of microorganisms grown on agar media (Masi et al, 2023). The results on plates from the 10 -5 graded dilution showed the best visibility of bacterial colonies where each colony formed was fully separated.…”

Section: Resultsmentioning

confidence: 99%

Detection and Pathogenicity Level Screening of Hemolytic Bacteria Isolated From Unpacked Chicken Nugget

Alfiyani,

Mukaromah,

Afriansyah

et al. 2023

lijhls

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Food poisoning is a severe threat that can lead to death. According to the WHO, cases of food poisoning have been on the rise in recent years. Pathogenic bacteria are responsible for food poisoning, often originating from contaminated food. Chicken nuggets, a popular ready-to-eat food, are susceptible to bacterial growth due to the risk of microbial contamination during storage. This study aims to assess the pathogenicity levels of bacteria in chicken nuggets when stored at room temperature for seven days. Samples from chicken nuggets left at room temperature were diluted and cultured on Nutrient Agar (NA) media. Subsequently, bacterial pathogenicity screening was conducted using MacConkey Agar (MC), Blood Agar Plate (BAP), and Chocolate Agar Plate (CAP). The results revealed six bacterial isolates labeled FMCN-1 to FMCN-6 (FMCN denoting Fatmawati Market chicken nugget). Most isolates exhibited a moderate level of pathogenicity (FMCN-2, FMCN-3, FMCN-4, FMCN-5, and FMCN-6), with only one isolate demonstrating low pathogenicity (FMCN-1). In conclusion, storing chicken nuggets at room temperature for seven days can lead to bacterial contamination with mostly moderate pathogenicity. Further molecular analysis is required to identify the potentially harmful bacteria.

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“…In October 2021, we isolated a strain in saline-alkali soil sample from Ningxia Autonomous Region, China. The soil sample was first cleared of debris and filtered using a 100-mesh screen, then washed and rinsed three times with sterile water [23]. Subsequently, the soil was diluted 10×, 10 2 ×, 10 3 ×, 10 4 ×, 10 5 ×, 10 6 ×, 10 7 ×, and 10 8 × with sterile water and inoculated (100 µL) onto potato dextrose agar (PDA) using the spread plate technique [24,25].…”

Section: Experimental Procedures 21 Isolation and Identification Of T...mentioning

confidence: 99%

Discovery, Identification, and Insecticidal Activity of an Aspergillus flavus Strain Isolated from a Saline–Alkali Soil Sample

Song,

Liu,

Feng

et al. 2023

Microorganisms

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Aphids are one of the most destructive pests in agricultural production. In addition, aphids are able to easily develop resistance to chemical insecticides due to their rapid reproduction and short generation periods. To explore an effective and environmentally friendly aphid control strategy, we isolated and examined a fungus with aphid-parasitizing activity. The strain (YJNfs21.11) was identified as Aspergillus flavus by ITS, 28S, and BenA gene sequence analysis. Scanning electron microscopy and transmission electron microscopy revealed that the infection hyphae of ‘YJNfs21.11’ colonized and penetrated the aphid epidermal layer and subsequently colonized the body cavity. Field experiments showed that ‘YJNfs21.11’ and its fermentation products exerted considerable control on aphids, with a corrected efficacy of 96.87%. The lipase, protease, and chitinase secreted by fungi help aphid cuticle degradation, thus assisting spores in completing the infection process. Additionally, changes were observed in the mobility and physical signs of aphids, with death occurring within 60 h of infection. Our results demonstrate that A. flavus ‘YJNfs21.11’ exhibits considerable control on Aphis gossypii Glover and Hyalopterus arundimis Fabricius, making it a suitable biological control agent.

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Isolation and characterization of potential multiple extracellular enzyme-producing bacteria from waste dumping area in Addis Ababa

Cited by 11 publications

References 44 publications

Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia

Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia

Detection and Pathogenicity Level Screening of Hemolytic Bacteria Isolated From Unpacked Chicken Nugget

Discovery, Identification, and Insecticidal Activity of an Aspergillus flavus Strain Isolated from a Saline–Alkali Soil Sample

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