Potential effect of hemoglobin solutions on blood components

Microcirculatory Function and Tissue Damage Is Improved After Therapeutic Injection of Bovine Hemoglobin in Severe Acute Rodent Pancreatitis

“…20 Somewhat surprising is the fact that HBOC-200 improved microcirculation despite its described effect of hyperaggregability, which might lead to leukocyte activation and adherence. 22,23 In contrast, this study is in accordance to previous studies that showed no increased expression of adherence receptors. 24 The clinical potential is also reflected by histologic assessment.…”

In vivo effects of Hb solutions on blood viscosity and rheologic behavior of RBCs: comparison with clinically used volume expanders

“…The most commonly accepted paradigm for the vasoactive effect of the HbOCs is that the heme moiety of HbOCs will trap endothelium‐derived relaxing factor or nitrous oxide and thus result in vasoconstriction, 28 although this has recently been shown to play only a minor role 29 . We present here evidence that the chemical modification of the hemoprotein induces significant effects on the plasma and whole‐blood viscosity as well as on RBC aggregation, as recently suggested 29 .…”

Effects of three Hb‐based oxygen‐carrying solutions on neutrophil activation in vitro: quantitative measurement of the expression of adherence receptors

“…Some HbOCs have been observed to induce modifications of the in vitro rheologic behavior of RBCs, such as hyper‐aggregability 2,3 . To further characterize the action of these solutions on blood cells, it is therefore desirable to evaluate whether Hb solutions have an in vitro effect on WBCs, and particularly on neutrophils.…”

“…Understanding the impact of the different HbOCs on peripheral blood cells is desirable, as some HbOCs are undergoing Phase III clinical trials and may shortly become therapeutic agents for use in humans. Previous studies have shown that Hb solutions produced some in vitro modifications in RBC behavior, including changes in aggregation tendency 2,3 . However, the influence of Hb solutions on WBC activation and behavior has not been extensively investigated, although in vitro studies have shown that cell‐free Hb stimulates MNCs, leading to the release of IL‐6, IL‐8, and TNFα and to an increase in their procoagulant activity 4,5 …”