Microcirculatory Function and Tissue Damage Is Improved After Therapeutic Injection of Bovine Hemoglobin in Severe Acute Rodent Pancreatitis

Strate, Tim; Mann, Oliver; Kleinhans, Helge; Rusani, Skelkim; Schneider, Claus; Yekebas, Emre F.; Freitag, M.; Standl, Thomas; Bloechle, C.; Izbicki, Jakob R.

doi:10.1097/01.mpa.0000157481.22155.2d

Cited by 55 publications

(36 citation statements)

References 23 publications

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“…Red blood cell count and hematocrit are among the prognostic markers of acute pancreatitis (22,24); therefore, a decrease in the abovementioned biochemical parameters in the control group was expected. The increase in red blood cell count as well as in hematocrit and hemoglobin levels after everolimus treatment suggests an improvement of tissue oxygenation (25) and of microcirculatory function (26). A decrease in blood urea nitrogen level is a rapid and reliable prognostic marker for acute pancreatitis (22).…”

Section: Discussionmentioning

confidence: 99%

Effects of everolimus on a rat model of cerulein-induced experimental acute pancreatitis

Özkardeş¹,

Bozkurt²,

Dumlu³

et al. 2015

UCD

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Objective: To analyze the biochemical and histopathological effects of everolimus in an experimental rat model of cerulein-induced acute pancreatitis. The aim of the present study was to determine the effects of everolimus on blood biochemical parameters and tissue histopathology in an experimental rat model of cerulein-induced acute pancreatitis. Material and Methods:In 30 Wistar albino rats (male; 240-260 g), acute pancreatitis was induced by an intraperitoneal injection of cerulein (50 μg/kg) administered twice in 2 h. They were equally divided into the following three groups: 0.9% isotonic solution (Group 1; control), everolimus once (Group 2), and everolimus twice (Group 3) by oral gavage after cerulein injection. Thirty hours after the induction of pancreatitis, blood samples were collected by direct intracardiac puncture, rats were sacrificed, and pancreatic tissue samples were obtained.Results: Biochemical analyses of the blood samples showed statistically significant difference in red blood cell count as well as hemoglobin, hematocrit, urea, and alanine transaminase levels among the study groups (p<0.05 in all). Everolimus proved to significantly increase red blood cell count in a dose-independent manner. Hemoglobin and hematocrit levels significantly increased only after treatment with one dose of everolimus. Urea level was significantly different between the Groups 2 and 3; however, no change was observed in both groups when compared with the control. Alanine transaminase level significantly decreased only after treatment with two doses of everolimus. Histopathological analyses revealed that everolimus significantly decreased inflammation and perivascular infiltrate in a dose-dependent manner (35% in Group 2, 75% in Group 3; p=0.048). Conclusion:Treatment with two doses of everolimus improved some biochemical and histopathological parameters of experimental rat models of cerulein-induced acute pancreatitis and implied the specific inhibition of inflammatory response pathways. Keywords: Pancreatitis, cerulein, everolimus, inflammation INTRODUCTIONAcute pancreatitis is a localized inflammatory condition with a high mortality rate (2.1%-9.2%) worldwide. Because of its potential to trigger systemic inflammatory response, it can cause multiple organ failure (1, 2). The common causes of acute pancreatitis are alcohol abuse or gallstone disease (60%-75% of the cases) followed by hypertriglyceridemia, hyperparathyroidism, pancreatic malignancy, endoscopic retrograde cholangiopancreatography, trauma, infectious agents (increasingly associated with HIV infection), drugs (such as tetracycline, azathioprine, ethinyl estradiol, mercaptopurine, and sulfamethoxazole), autoimmunity, abdominal trauma, ischemia, diabetes mellitus, porphyria, and heredity (3-5).Although the disease is common, the treatment of acute pancreatitis is still very limited. There are only a number of pharmacological approaches, which are mostly supportive involving adequate fluid resuscitation and oxygen supplementation, to maintain normal art...

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Section: Discussionmentioning

confidence: 99%

Effects of everolimus on a rat model of cerulein-induced experimental acute pancreatitis

Özkardeş¹,

Bozkurt²,

Dumlu³

et al. 2015

UCD

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“…A portion of pancreatic tissue was fixed in 10% neutral formalin fixative solution for histological studies. After fixation tissues were embedded in paraffin, solid sections were cut at 5 µm and the sections were stained with haematoxylin and eosin [17] .…”

Section: Histopathology Of Pancreasmentioning

confidence: 99%

Histological changes and antidiabetic activities of Icacina trichantha tuber extract in beta–cells of alloxan induced diabetic rats

Monday

Uzoma

2013

Asian Pacific Journal of Tropical Biomedicine

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“…Intravascular volume depletion leads to hemoconcentration (hematocrit ≥44), tachycardia, hypotension, scanty urine output, and prerenal azotemia. There is abundant experimental evidence that early aggressive fluid resuscitation and improved delivery of oxygen prevent or minimize pancreatic necrosis and improve survival (176)(177)(178). Although comparable studies have not been carried out in clinical practice, there is widespread acceptance of the importance of aggressive fluid resuscitation in acute pancreatitis.…”

Section: Treatment Guideline I: Supportive Carementioning

confidence: 99%

Practice Guidelines in Acute Pancreatitis

Banks

Freeman

2006

Am J Gastroenterology

1,662

980

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Microcirculatory Function and Tissue Damage Is Improved After Therapeutic Injection of Bovine Hemoglobin in Severe Acute Rodent Pancreatitis

Abstract: Therapeutic intravenous infusion of bovine hemoglobin improves pancreatic microcirculation and reduces pancreatic tissue damage in severe acute rodent pancreatitis but is not as effective as early (prophylactic) administration.

Cited by 55 publications

References 23 publications

Effects of everolimus on a rat model of cerulein-induced experimental acute pancreatitis

Effects of everolimus on a rat model of cerulein-induced experimental acute pancreatitis

Histological changes and antidiabetic activities of Icacina trichantha tuber extract in beta–cells of alloxan induced diabetic rats

Practice Guidelines in Acute Pancreatitis

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