Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition

Martı́nez, Silvina Pérez; Conesa, Daniela; Cuasnicú, Patricia S.

doi:10.1016/0165-0378(95)00927-d

Cited by 50 publications

(29 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The preparation of sperm and tissue extracts was performed as previously described elsewhere (18). Extracted proteins were separated by SDS-PAGE along with broad range protein markers (Life Technologies, Rockville, MD).…”

Section: Electrophoresis and Western Blotmentioning

confidence: 99%

“…Results indicated that active immunization of male and female rats with purified DE raised specific anti-DE antibodies in most animals and produced a significant and reversible inhibition of fertility in both sexes (17). Subsequent studies revealed that this inhibition would be mediated by the entry of antibodies into the reproductive tract and their specific interference with the sperm fertilizing ability (18,19), supporting the use of this epididymal protein for the development of an immunocontraceptive method.…”

mentioning

confidence: 97%

See 1 more Smart Citation

Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives

Ellerman

Busso

Maldera

et al. 2008

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

Section: Electrophoresis and Western Blotmentioning

confidence: 99%

mentioning

confidence: 97%

Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives

Ellerman

Busso

Maldera

et al. 2008

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

“…The evidence came from in vivo observations showing that rat sperm exposed to a serum against rCRISP1 prior to uterine insemination exhibited a significantly reduced fertilizing ability (Perez Martinez et al, 1995). Interestingly, the non-fertilized eggs did not accumulate sperm in the perivitelline space as expected for the blocking of a protein involved in gamete fusion, suggesting that the antirCRISP1 antibodies might have interfered with an event prior to sperm-egg fusion.…”

Section: Epididymal Crisp1mentioning

confidence: 99%

Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction

Cohen¹,

Busso²,

Ros³

et al. 2008

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Mammalian fertilization is a complex multi-step process mediated by different molecules present on both gametes. CRISP1 (cysteine-rich secretory protein 1) is an epididymal protein thought to participate in gamete fusion through its binding to egg-complementary sites. Structure-function studies using recombinant fragments of CRISP1 as well as synthetic peptides reveal that its egg-binding ability resides in a 12 amino acid region corresponding to an evolutionary conserved motif of the CRISP family, named Signature 2 (S2). Further experiments analyzing both the ability of other CRISP proteins to bind to the rat egg and the amino acid sequence of their S2 regions show that the amino acid sequence of the S2 is needed for CRISP1 to interact with the egg. CRISP1 appears to be involved in the first step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. The observation that sperm testicular CRISP2 is also able to bind to the egg surface suggests a role for this protein in gamete fusion. Subsequent experiments confirmed the participation of CRISP2 in this step of fertilization and revealed that CRISP1 and CRISP2 interact with common egg surface binding sites. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization. These observations contribute to a better understanding of the molecular mechanisms underlying mammalian fertilization.

show abstract

“…Although some studies show reduced sperm-zona binding in vitro, males retained fertility. [5][6][7] Although these studies, and the Crisp1 knockout mouse, suggest that the collective inhibition of all CRISPs may form the basis of a contraceptive, immunocontraceptives are unlikely to be an effective and acceptable mode of action.…”

Section: Crisps and Male Infertilitymentioning

confidence: 99%

“…Not surprisingly, therefore, the CRISP genes have been nominated as infertility candidate genes and proposed as targets for contraceptive action. [5][6][7] This article will review current literature on the role of CRISPs in male fertility; it will draw upon the function of related non-mammalian CRISPs and other related proteins to infer function and finally will summarize the actual evidence for the role of CRISPs in mammalian male fertility.…”

Section: Introductionmentioning

confidence: 99%

The role of cysteine-rich secretory proteins in male fertility

Koppers¹,

Reddy²,

O'Bryan³

2010

Asian J Androl

View full text Add to dashboard Cite

The cysteine-rich secretory proteins (CRISPs) are a subgroup of the CRISP, antigen 5 and Pr-1 (CAP) protein superfamily, and are found only in vertebrates. They show a strong expression bias to the mammalian male reproductive tract and the venom of poisonous reptiles. Within the male reproductive tract CRISPs have been implicated in many aspects of male germ cell biology spanning haploid germ cell development, epididymal maturation, capacitation, motility and the actual processes of fertilization. At a structural level, CRISPs are composed of two domains, a CAP domain, which has been implicated in cell-cell adhesion, and a CRISP domain, which has been shown to regulate several classes of ion channels across multiple species. Herein, we will review the current literature on the role of CRISPs in male fertility, and by inference to related non-mammalian protein, infer potential biochemical functions.

show abstract

Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition

Cited by 50 publications

References 25 publications

Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives

Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives

Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction

The role of cysteine-rich secretory proteins in male fertility

Contact Info

Product

Resources

About