Potential Biomarkers of Skeletal Muscle Damage

Rebalka, Irena A.; Hawke, Thomas J.

doi:10.2217/bmm.13.163

Cited by 16 publications

(12 citation statements)

References 22 publications

(25 reference statements)

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“…Although aminotransferases are widely applied in the clinic as sensitive biomarkers for hepatocyte injury, the concurrence of increased serum CK and aminotransferases is indicative for muscle injury in most cases in dystrophinopathy [ 20 , 42 , 43 ]. However, the elevated levels of aminotransferases in muscular dystrophy sometimes make clinicians confused, which leads to the misdiagnosis of liver diseases and unnecessary liver biopsy [ 20 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…CK is the well-known biomarker closely associated with muscle injury and muscular dystrophy, and elevated levels of CK can differentiate some types of muscular dystrophy [ 43 ]. Thus, we chose CK to adjust the baselines of aminotransferases in this study.…”

Section: Discussionmentioning

confidence: 99%

“…In patients with dystrophinopathy, the elevated levels of CK and aminotransferases can vary with age and other factors, such as genotype, clinical phenotype, and motor function, all of which made it difficult to establish applicable baselines of CK and aminotransferase levels for diagnostic purposes [ 43 ]. Interestingly, we found that CK-adjusted ALT could diminish the variability associated with age and other factors correlated with muscle injury, which suggests that CK/ALT is less influenced by muscle injury and has the possibility of application for dystrophinopathy.…”

Section: Discussionmentioning

confidence: 99%

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Ratio of Creatine Kinase to Alanine Aminotransferase as a Biomarker of Acute Liver Injury in Dystrophinopathy

Wang

Chen

et al. 2018

Disease Markers

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Objective To investigate the ratios of creatine kinase (CK) to aminotransferases as biomarkers of acute liver injury in dystrophinopathy. Methods C57 and mdx (dystrophic) mice were treated with a hepatotoxic reagent D-galactosamine (D-GalN). The degrees of liver and muscle injury were assessed using histological examinations. To examine whether serum CK-adjusted aminotransferase levels could indicate liver status in dystrophic mice, the CK/alanine aminotransferase (ALT) and CK/aspartate aminotransferase (AST) ratios were analyzed. Furthermore, we enrolled 658 male patients with dystrophinopathy and 378 male patients without muscle and liver injury as control, whose serum ALT, AST, and CK levels were examined. Results Animal experiments indicated that D-GalN treatment could induce acute liver injury but not muscle injury. Additionally, D-GalN decreased the CK/ALT and CK/AST ratios in both C57 mice and mdx mice (P < 0.001). However, there was an overlap of the CK/AST ratio between dystrophic mice with and without acute liver injury. In patients with dystrophinopathy, CK-adjusted ALT diminished the variability associated with age, genotype, clinical phenotype, and motor function (P > 0.05). Conclusions CK/ALT is a potential biomarker for the differential evaluation of acute liver injury in dystrophic mice, which highlights the value to further evaluate the practice of CK/ALT in dystrophinopathy patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Ratio of Creatine Kinase to Alanine Aminotransferase as a Biomarker of Acute Liver Injury in Dystrophinopathy

Wang

Chen

et al. 2018

Disease Markers

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“…Routine clinical biofluid analyses in the field of muscle pathology are usually restricted to simple blood tests with a focus on muscle-derived serum proteins that are indicative of skeletal muscle damage, including creatine kinase, myoglobin, alanine aminotransferase, aspartate aminotransferase and a variety of glycolytic enzymes [40] . However, in the case of childhood disorders of the neuromuscular system, these serum markers are relatively non-specific and potentially susceptible to (i) modifying effects due to co-morbidities, (ii) considerable age-related changes during childhood development, (iii) fluctuations during disease progression, (iv) obscuring influences of patient gender and ethnicity, (v) prone to changes due to general life style, nutritional status, metabolic imbalances and physical activity levels, (vi) modifying effects of intramuscular injections, surgery or drug treatments, and (vii) seasonal variations in concentration levels [4] .…”

Section: Resultsmentioning

confidence: 99%

Proteomic identification of elevated saliva kallikrein levels in the mdx-4cv mouse model of Duchenne muscular dystrophy

Murphy

Zweyer

Mundegar

et al. 2019

Biochemistry and Biophysics Reports

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Dystrophinopathies are multi-system disorders that affect the skeletal musculature, the cardio-respiratory system and the central nervous system. The systematic screening of suitable biofluids for released or altered proteins promises new insights into the highly complex pathophysiology of X-linked muscular dystrophy. However, standard detection approaches using antibody-based assays often fail to reproducibly detect low-abundance protein isoforms in dilute biological fluids. In contrast, mass spectrometric screening approaches enable the proteome-wide identification of minor protein changes in biofluids. This report describes the findings from the comparative proteomic analysis of whole saliva samples from wild type versus the established mdx-4cv mouse model of highly progressive muscular dystrophy, focusing on the kallikrein protein family. Kallikrein-1 (Klk1) and 13 Klk1-related peptidases were identified in saliva and serum from normal mice. Comparative proteomics revealed elevated saliva levels of the Klk1-related peptidases Klk1-b1, Klk1-b5 and Klk-b22, as well as an increased Klk-1 concentration, which agrees with higher Klk-1 levels in serum from mdx-4cv mice. This indicates altered cellular signaling, extracellular matrix remodeling and an altered immune response in the mdx-4cv mouse, and establishes liquid biopsy procedures as suitable bioanalytical tools for the systematic survey of complex pathobiochemical changes in animal models of muscular dystrophy.

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“…Studies have reported that after the rupture of skeletal muscle cell membranes caused by injuries, serum levels of proteins such as creatine kinase (CK) and lactate dehydrogenase (LHD) leak out into the bloodstream 7 , but the existence of three CK isoforms present in large release sites impairs the use of this enzyme alone as an indicator of overtraining 8 . The interaction of physical fitness on psychophysiological responses demonstrates that individuals who present better aerobic conditioning obtain lower physiological responses to stress 9,10 , thus proving to be a determinant of athlete performance.…”

Section: Introductionmentioning

confidence: 99%

Psychometric, Anthropometric and Biochemical Profile of Mountain Bike Amateur Cyclists: A Longitudinal Study

Andrade¹,

Santos

Aniceto

et al. 2018

J Phys Educ

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Atletas praticantes de mountain bike podem desenvolver problemas relacionados ao excesso de treinamento, ocasionando perda de desempenho. O objetivo foi analisar as variáveis psicométricas, bioquímicas e composição corporal de ciclistas amadores que praticam o mountain bike. Participaram 10 ciclistas ativos a dois anos. Registrou-se as variáveis no 1º, 15º e 30º dia durante um mês. Por meio dos questionários POMS e REST-Q foram avaliados estado de humor e estresserecuperação, por meio do percentual e quilograma de gordura, e massa magra, analisou-se a composição corporal, por meio das enzimas creatinaquinase (CK) e lactato desidrogenase (LDH) avaliou o dano muscular. Para a análise foi utilizada ANOVA/medidas repetidas. Observou-se que os voluntários estavam com índices significativos de tensão, depressão e hostilidade no 15º e 30º dia em relação ao 1º (p<0.05); relataram menor conflito/pressão e exaustão emocional no 15º dia em relação ao 1º (p<0.05). Não foram encontradas diferenças significantes na atividade das enzimas LDH e CK, e em nenhum dos componentes de composição corporal no 30º dia em relação ao primeiro (p<0.05). Conclui-se que o treinamento realizado sem monitoramento de um profissional que prescreva e o acompanhe diariamente pode acarretar em alterações psicométricas importantes e indicativas de overtraining. Palavras-chave: Atletas. Educação Física e treinamento. Metabolismo.

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Potential Biomarkers of Skeletal Muscle Damage

Cited by 16 publications

References 22 publications

Ratio of Creatine Kinase to Alanine Aminotransferase as a Biomarker of Acute Liver Injury in Dystrophinopathy

Ratio of Creatine Kinase to Alanine Aminotransferase as a Biomarker of Acute Liver Injury in Dystrophinopathy

Proteomic identification of elevated saliva kallikrein levels in the mdx-4cv mouse model of Duchenne muscular dystrophy

Psychometric, Anthropometric and Biochemical Profile of Mountain Bike Amateur Cyclists: A Longitudinal Study

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