Potential application of algicidal bacteria for improved lipid recovery with specific algae

Abstract

The utility of specific strains of natural algicidal bacteria isolated from shallow wetland sediments was evaluated against several strains of algae with potential immediate or future commercial value. Two strains of bacteria, Pseudomonas pseudoalcaligenes AD6 and Aeromonas hydrophila AD9, were identified and demonstrated to have algicidal activity against the microalgae Neochloris oleoabundans and Dunaliella tertiolecta. These bacteria were further evaluated for the potential to improve lipid extraction using a mild solvent extraction approach. Aeromonas hydrophila AD9 showed a nearly 12-fold increase in lipid extraction with D. tertiolecta, while both bacteria showed a sixfold improvement in lipid extraction with N. oleoabundans.

Gene Deletions Resulting in Increased Nitrogen Release by Azotobacter vinelandii: Application of a Novel Nitrogen Biosensor

“…Cultures of Chlorella sorokiniana UTEX 1602 were obtained from the UTEX culture collection of algae (Austin, TX) and have been maintained for several years by subculturing on solid media (11). Algae strains were cultured in a freshwater medium as described previously (26). Algal cells in solution were measured using a hemocytometer following the directions of the manufacturer (Hausser Scientific, Horsham, PA).…”

Green microalgae biomolecule separations and recovery

“…Interestingly, the co-cultured organisms can be isolated from known microalgae predators and previous work has demonstrated a degree of selectivity and specificity that can be achieved using predator-derived organisms. Chlorella, Nannochloropsis, and Dunaliella species have been successfully disrupted using this technique (Chen et al 2013a, b;Lenneman et al 2014). Advantages of algicidal lysis include cost, elimination of need for external enzyme application during downstream processing, high selectivity, and mild extraction conditions (pH, temperature, etc.)…”

Algicidal microorganisms and secreted algicides: New tools to induce microalgal cell disruption

“…Furthermore, mutualistic and allelopathic interaction between bacteria and microalgae have recently been highlighted for applications in biofuels production processes as a potential strategy to control culture contamination during algal cultivation (Bacellar Mendes and Vermelho 2013), to fast-sedimentation of microalgae by bacterial aggregation and to disrupt microalgae biomass using algicidal microorganisms Cheng et al 2013;Lenneman et al 2014;Lü et al 2013;Muñoz et al 2014;Wang and Yuan 2014).…”

“…For the purpose of biofuel production, the selected alga-lytic microorganisms have been isolated from different ecosystems. They have been collected from the natural environment of marine microalgae in order to reach cell lysis in saline conditions (Lenneman et al 2014, Matsumuto et al 2003Muñoz et al 2014) or isolated from heterogeneous environmental samples recovered in contaminated open ponds or shallow marsh sediments (Lenneman et al 2014). Cellulolytic bacteria were also isolated from digestive guts of algae predators such as filter-feeding bivalve mollusks (Muñoz et al 2014), and from lignin-rich, pulp mill effluent (Wang and Yuan 2014).…”

“…Cellulolytic bacteria were also isolated from digestive guts of algae predators such as filter-feeding bivalve mollusks (Muñoz et al 2014), and from lignin-rich, pulp mill effluent (Wang and Yuan 2014). Specific microalgae cell wall damage showed evidence of the effectiveness of biological treatment (Afi et al 1996;Chen et al 2013;Lenneman et al 2014;Lü et al 2013). One of the major reasons for the damage to microalgal cell wall was reported to be the enzymatic degradation, which was confirmed by the detection of enzymatic activities in co-culture supernatants (Afi et al 1996;Chen et al 2013;Cheng et al 2013;Lü et al 2013;Matsumoto et al 2003;Muñoz et al 2014).…”