Potential antioxidant bioactive peptides from camel milk proteins

Abstract

Camel milk is traditionally considered to have medicinal characteristics that it has potential health benefits and could help to treat several illnesses. Particularly, it is closest to human breast milk and has high levels of nutrients and bioactive components. The aim of this study was to explore the antioxidant peptides derived from protein fractions of camel milk. Camel milk proteins (CMP) were fractionated into camel casein protein (CCP) and camel whey protein (CWP), which were hydrolyzed with pepsin to produce peptic digests P-CCP and P-CWP, respectively. RP-HPLC was used for fractionation of the peptides from the P-CCP and P-CWP. The antioxidant activities were evaluated using superoxide anion generating system of xanthine oxidase (XOD) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Active peptides were analyzed using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) whereas a number of antioxidant peptides, with masses ranging from 913 to 2,951 Da, derived mainly from alpha-casein, lactophorin and lactoferrin, were identified. When yeast cells are used as a system for modeling mitochondrial disease, the peptides in caseins and whey fractions significantly enhanced the tolerance of yeast cells against peroxide-induced oxidative stress. The results show that both caseins and whey proteins of camel milk possess bioactive peptides with significant radical-scavenging activities and thus herald a fascinating opportunity for their potential as nutraceuticals or therapeutic peptides for prevention and treatment of oxidative stress-associated diseases.

Radioprotective Effect of Walnut Oligopeptides Against Gamma Radiation-Induced Splenocyte Apoptosis and Intestinal Injury in Mice

“…Antioxidants are known to scavenge free radicals, and therefore considered strong candidates for development of radioprotector [16]. The antioxidant capacities of bioactive peptides, in which different amino acid compositions have different validities, have been extensively studied [8,17,18]. Previous studies demonstrated that walnut protein hydrolysate was an effective antioxidant for scavenging various free radicals through different scavenging ways and make it a potential therapeutic agent for alleviation of memory impairment [19,20].…”

Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma

“…Our results are consistent with the literature. Recently, Ibrahim et al (2018) studied potential antioxidant bioactive proteins from CM proteins. Reversed-phase HPLC was used for the fractionation of peptides.…”

Exploring Potential Bioactive Peptides in Fermented Bactrian Camel’s Milk and Mare’s Milk Made by Mongolian Nomads

“…On the other hand, the lactophorin-derived peptides, R 75 RHQNQNPK 83 and R 76 HQNQNPK 83 , found in this study were embedded in the middle part of mature lactophorin. Ibrahim et al [38] recently found two lactophorin-derived peptides, E 113 NTMRETMDFLKSLF 127 and A 79 TTLEGKLVEL 89 , in pepsin-digested camel milk whey hydrolysates that confer tolerance against H 2 O 2 -induced oxidative stress to yeast cells. The biological significance of the lactophorin-derived peptides found in the fermented camel's milk in this study remains to be elucidated.…”