1990
DOI: 10.1016/0168-1656(90)90049-h
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Potential and problems of animal cells in suspension culture

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Cited by 34 publications
(10 citation statements)
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“…Serum is routinely added to the cryopreservation media as a source of nutrients, and other undefi ned factors, in spite of scientifi c and technical disadvantages to its inclusion, its high cost, and the increasing availability of serum-free alternatives [17][18][19][20]. Obviously, for in vivo clinical use, the elimination of animal serum proteins and all possible sources of infectious diseases such as hepatitis, human immunodefi ciency virus (HIV), and bovine spongiform encephalopathy (BSE) is a prerequisite [21][22][23].…”
Section: Introductionmentioning
confidence: 99%
“…Serum is routinely added to the cryopreservation media as a source of nutrients, and other undefi ned factors, in spite of scientifi c and technical disadvantages to its inclusion, its high cost, and the increasing availability of serum-free alternatives [17][18][19][20]. Obviously, for in vivo clinical use, the elimination of animal serum proteins and all possible sources of infectious diseases such as hepatitis, human immunodefi ciency virus (HIV), and bovine spongiform encephalopathy (BSE) is a prerequisite [21][22][23].…”
Section: Introductionmentioning
confidence: 99%
“…MSCs encapsulated in 3‐D porous microcarrier beads are protected from shear stresses, although diffusion of oxygen and nutrients within beads can be limited. Furthermore, one challenge associated with microcarrier culture is optimization of cell‐harvesting protocols to guarantee efficient cell‐bead separation and high cell recovery without compromising viability or functionality . (3) MSCs cultured on 3‐D matrices fabricated using polymers, ceramic materials, or decellularized matrices .…”
Section: Introductionmentioning
confidence: 99%
“…Packed‐bed and hollow fiber bioreactors have been commonly used for perfusion culture of immobilized mammalian cells 7. High cell densities of 10 7 –10 8 cells per milliliter can be easily achieved in immobilized cell cultures, extending the productive lifetime of cells and resulting in increased mAb productivity 8, 9. Disadvantages of perfusion culture include their complex nature and difficulty in scale up.…”
Section: Introductionmentioning
confidence: 99%