Potential action of copper surfaces on meticillin-resistant Staphylococcus aureus

Weaver, Louise; Noyce, J.O.; Michels, Harold T.; Keevil, C. W.

doi:10.1111/j.1365-2672.2010.04852.x

Cited by 120 publications

(103 citation statements)

References 26 publications

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“…wound dressings or as a material for common hospital surfaces [9,10]. In addition, copper incorporated in paints has also been shown to inhibit biofilm formation of bacteria and could thus be employed as an antifouling agent [11].…”

Section: Introductionmentioning

confidence: 99%

The antimicrobial and antibiofilm activities of copper(II) complexes

Beeton

Aldrich‐Wright

Bolhuis

2014

Journal of Inorganic Biochemistry

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Gram-positive and Gram-negative bacteria, albeit that activity was generally higher for the former. The antibiofilm activity was then determined against a clinical isolate of meticillin-resistant Staphylococcus aureus (MRSA). Strikingly, the copper complexes tested showed significant activity against biofilms, and were better in the removal of biofilms than vancomycin, an antibiotic that is currently used in the treatment of MRSA infections.

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Section: Introductionmentioning

confidence: 99%

The antimicrobial and antibiofilm activities of copper(II) complexes

Beeton

Aldrich‐Wright

Bolhuis

2014

Journal of Inorganic Biochemistry

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“…Resistance to host-mediated copper killing of Escherichia coli (3), Salmonella enterica (4), and Mycobacterium tuberculosis (5,6) and sulfur assimilation and cysteine biosynthesis in M. tuberculosis (7,8) are two such processes. S. aureus is particularly sensitive to rapid killing when exposed to copper or copper alloy surfaces, justifying this therapeutic direction (9,10).…”

mentioning

confidence: 99%

Control of Copper Resistance and Inorganic Sulfur Metabolism by Paralogous Regulators in Staphylococcus aureus

Grossoehme

Kehl-Fie

et al. 2011

Journal of Biological Chemistry

214

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All strains of Staphylococcus aureus encode a putative copper-sensitive operon repressor (CsoR) and one other CsoR-like protein of unknown function. We show here that NWMN_1991 encodes a bona fide Cu(I)-inducible CsoR of a genetically unlinked copA-copZ copper resistance operon in S. aureus strain Newman. In contrast, an unannotated open reading frame found between NWMN_0027 and NWMN_0026 (denoted NWMN_0026.5) encodes a CsoR-like regulator that represses expression of adjacent genes by binding specifically to a pair of canonical operator sites positioned in the NWMN_0027-0026.5 intergenic region. Inspection of these regulated genes suggests a role in assimilation of inorganic sulfur from thiosulfate and vectorial sulfur transfer, and we designate NWMN_ 0026.5 as CstR (CsoR-like sulfur transferase repressor). Expression analysis demonstrates that CsoR and CstR control their respective regulons in response to distinct stimuli with no overlap in vivo. Unlike CsoR, CstR does not form a stable complex with Cu(I); operator binding is instead inhibited by oxidation of the intersubunit cysteine pair to a mixture of disulfide and trisulfide linkages by a likely metabolite of thiosulfate assimilation, sulfite. CsoR is unreactive toward sulfite under the same conditions. We conclude that CsoR and CstR are paralogs in S. aureus that function in the same cytoplasm to control distinct physiological processes.The Gram-positive opportunistic human pathogen Staphylococcus aureus is the causative agent of a wide range of hospital and community-acquired infections that are associated with significant morbidity (1). With the incidence of methicillinresistant strains increasing in previously low prevalence areas (2), new antibiotic therapies that target novel metabolic pathways are urgently needed. One approach is to target those processes that allow a pathogen to respond to environmental stresses that might change depending on the microenvironmental host niche in which the organism finds itself. Resistance to host-mediated copper killing of Escherichia coli (3), Salmonella enterica (4), and Mycobacterium tuberculosis (5, 6) and sulfur assimilation and cysteine biosynthesis in M. tuberculosis (7,8) are two such processes. S. aureus is particularly sensitive to rapid killing when exposed to copper or copper alloy surfaces, justifying this therapeutic direction (9, 10).M. tuberculosis CsoR 6 (copper-sensitive operon repressor) is a founding member of large family of regulators now known collectively to respond to Cu(I), Ni(II), and perhaps other stressors, the structural basis of which is not fully understood (11, 12). All CsoR family proteins lack a known canonical DNA binding domain and are projected to adopt the flat disc-shaped dimer of dimers homotetrameric structure characteristic of Cu(I)-sensing CsoRs, with individual dimers consisting of an antiparallel four-helix bundle flanked by a C-terminal ␣3 helix (13,14). Two cysteine residues on opposite subunits within a dimer make coordination bonds to the Cu(I) ion, with the third ...

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“…Other researchers observed that Cu could displace Fe atoms from dehydratase Fe-S clusters and block branched-chain amino acid synthesis in E. coli 37) . Various scientific studies account for the beneficial use of copper and its alloys, most notably with its multitoxicity, which also renders copper effective against multiresistant germs like the "superbug" MRSA (methicillin resistant Staphylococcus aureus) 38) or carbapenemase-resistant bacteria 39) . On the other hand, we know that a high concentration of Cu can cause upset stomach, nausea and diarrhea and can lead to tissue injury and disease 40) .…”

Section: Discussionmentioning

confidence: 99%

The anti-bacterial activity of titanium-copper sintered alloy against <i>Porphyromonas gingivalis</i> <i>in vitro </i>

et al. 2016

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This study investigates the anti-bacterial property of Ti-Cu sintered alloys against Porphyromonas gingivalis. The anti-anaerobic property of Ti-Cu sintered alloys against P. gingivalis was investigated by antibacterial activity test, DNA measurement, DAPI staining and morphology observation. The antibacterial rates of the Ti-5Cu against P. gingivalis after 18 and 24 h incubation were 36.04 and 54.39%, and those of Ti-10Cu were 68.69 and 75.39%, which were lower than their anti-aerobic abilities. The concentration of P. gingivalis DNA gradually decreased with the increasing Cu content, which was nearly 50% after 24 h incubation on Ti-10Cu. SEM results showed that the shape of P. gingivalis changed and the bacteria broke apart with the addition of Cu and the extension of the culture time. Ti-Cu sintered alloys could not only kill anaerobic bacteria but also reduce the activity of the survived bacteria. The anti-anaerobic mechanism was thought to be in associated with the Cu ion released from Ti-Cu alloy.

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Potential action of copper surfaces on meticillin-resistant Staphylococcus aureus

Cited by 120 publications

References 26 publications

The antimicrobial and antibiofilm activities of copper(II) complexes

The antimicrobial and antibiofilm activities of copper(II) complexes

Control of Copper Resistance and Inorganic Sulfur Metabolism by Paralogous Regulators in Staphylococcus aureus

The anti-bacterial activity of titanium-copper sintered alloy against <i>Porphyromonas gingivalis</i> <i>in vitro </i>

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