Potent therapeutic effects of ruscogenin on gastric ulcer established by acetic acid

Ercan, Gülçin; Tartar, Rümeysa İlbar; Solmaz, Ali; Gülçiçek, Osman Bilgin; Karagülle, Onur Olgaç; Meriç, Serhat; Çayören, Hüseyin; Kuşaslan, Ramazan; Kemik, Ahu Sarbay; Kayalı, Damla Gökçeoğlu; Çetınel, Şule; Çelik, Atilla

doi:10.1016/j.asjsur.2019.07.001

Cited by 28 publications

(17 citation statements)

References 29 publications

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“…In our previous study, we showed acetic acid-induced gastritis resulting in devastating damage on ultrastructure of chief and parietal cells while ruscogenin almost completely ameliorated these effects and preserved the ultrastructure, suggesting a correlation between cellular integrity and anti-inflammatory effects [19]. In this study, the ruscogenin treatment ameliorated the cerulein-induced ultrastructural degenerations in the endocrine and exocrine cells of pancreatic tissue, especially in the treatment group.…”

Section: Discussionsupporting

confidence: 55%

“…Two main active ingredients of this plant responsible from its pharmacological effects, ruscogenin and neoruscogenin recently gain attention for their anti-inflammatory and antioxidant characteristics, as well as their vasoconstrictive and venotonic features. In our previous study, we have also demonstrated that the ruscogenin treatment reduced ulceration in gastric tissues in a chronic ulcer model established by acetic acid induction [19]. The healing effects of ruscogenin in acetic acid-induced gastric ulcer may be due to inhibiting oxidative stress, promoting antioxidant mechanisms and the inhibition of lipid peroxidation by maintaining a balance in collagen content of extracellular cell matrix and ultrastructure [19].…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, we have also demonstrated that the ruscogenin treatment reduced ulceration in gastric tissues in a chronic ulcer model established by acetic acid induction [19]. The healing effects of ruscogenin in acetic acid-induced gastric ulcer may be due to inhibiting oxidative stress, promoting antioxidant mechanisms and the inhibition of lipid peroxidation by maintaining a balance in collagen content of extracellular cell matrix and ultrastructure [19]. In the present study, we discovered the healing and protective effects of ruscogenin in a cerulein-induced AP rat model by histopathological, immunohistochemical, biochemical, and electron microscopical analysis.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, ruscogenin was considered to exert its potent efficacy in gastric ulcer via anti-inflammatory actions through reducing TNF-α, IL-6 and IL-8, antioxidant actions through enhancing glutathione activities, in addition to depression in oxidative stress levels and suppressive effects on lipid metabolism [19]. In the present study, the efficient protective and therapeutic effects are probably due to anti-inflammatory actions through decreasing blood cytokine levels of CRP, hs-CRP levels, TNF-α, IL-6, IL-1β, ICAM-1, by reducing the pancreatic enzymes amylase and lipase in the blood, and by suppressing the expression of transcription factor NF-κB, and by staving off the augmentation in NO production through suppressing NOS1 in pancreatic tissues.…”

Section: Discussionmentioning

confidence: 99%

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Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

et al. 2019

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PurposeTo determine the potential protective and therapeutic effects and action mechanism of ruscogenin on cerulein-induced acute pancreatitis (AP) model in rats.MethodsOverall, 32 rats were attenuated to the sham (2-mL/kg/day isotonic solution for 4 weeks), control (20-µg/kg cerulein-induced AP for 12 hours), prophylaxis groups (cerulein-induced AP following 3-mL/kg/day ruscogenin for 4 weeks) and treatment (3-mL/kg/day ruscogenin following cerulein-induced AP for 12 hours). Blood samples were collected for biochemical analysis of nitric oxide synthase 1 (NOS1/neuronal NOS), malondialdehyde (MDA) and intercellular adhesion molecule 1 (ICAM-1). After sacrification, pancreas tissues were collected and prepared for light microscopic (hematoxylin and eosin), immunohistochemical (nuclear factor kappa B) and biochemical analysis (tumor necrosis factor-alpha [TNF-α], interleukin-6 and 1β [IL-6 and IL-1β], CRP, high-sensitivity CRP [hs-CRP] amylase, lipase, and ICAM-1). Ultrastructural analysis was performed by transmission electron microscopy.ResultsThe protective and therapeutic actions of ruscogenin were accomplished by improvements in histopathology, by decreasing blood cytokine levels of CRP, hs-CRP levels, TNF-α, IL-6, IL-1β, ICAM-1, by reducing the pancreatic enzymes amylase and lipase in blood, and by suppressing the expression of nuclear factor kappa B, ICAM-1, and NOS-1, but not MDA in pancreatic tissues. Ruscogenin also improved cerulein-induced ultrastructural degenerations in endocrine and exocrine cells, especially in treatment group.ConclusionThe present findings have demonstrated the beneficial protective and therapeutical effects of ruscogenin, nominating it as a highly promising supplementary agent to be considered in the treatment of AP, and even as a protective agent against the damages induced by disease.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

et al. 2019

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“…These increase in their expression levels usually mean the aggravation of body damage. IL-6 can activate the central nervous system to regulate injury and defense, and at the same time regulate immune response and acute phase response, which is significantly increased in patients with gastric ulcer [33]. TNF-α is a kind of endogenous multidirectional inflammatory cell factors that can activate neutrophils and continue to damage the gastric mucosa, causing ulcers to be difficult to heal [34].…”

Section: Discussionmentioning

confidence: 99%

Codonopsis Decoction Inhibits Aspirin-Induced Gastric Mucosal Injury in Rats by Regulating COX, COX-1, COX-2, TNF-α, IL-6 and TGF-α

Wang¹,

Lu²,

Deng³

et al. 2021

BJSTR

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Codonopsis (Codonopsis pilosula (Franch.) Nannf.) is a common medicinal and edible plant with a variety of biological functions, including enhancing gastrointestinal motility, anti-inflammatory, antioxidant, regulating glucose and lipid metabolism, regulating immune response and anti-tumor. Aspirin is a widely used clinical drug with multiple therapeutic effects. However, long-term use of aspirin could also bring some side effects, including gastrointestinal discomfort, liver and kidney damage, asthma, and allergies. The present study is aimed to investigate the effect of Codonopsis decoction on aspirin-induced gastric injuries. Cell proliferation was evaluated by MTT assay and cell apoptosis was investigated by flow cytometry. Gastric mucosal injury index and HE staining were used to evaluate the pathological changes of gastric tissue. The levels of cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and transforming growth factor-α (TGF-α) in GES-1 cells culture supernatant were detected by ELISA kits. The levels of cyclooxygenase (COX), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and TGF-α in gastric tissue and serum were detected by ELISA kits. Results showed that Codonopsis decoction significantly attenuated the aspirin-induced cytotoxicity and apoptosis of GES-1 cells. Codonopsis decoction significantly increased the levels of COX-1, COX-2 and TGF-α in GES-1 cells treated by aspirin. Codonopsis decoction could also increase the expression levels of COX and TGF-α in vivo. Codonopsis decoction significantly reduced the levels of TNF-α and IL-6 both in vivo and in vitro. In addition, Codonopsis decoction could increase the daily weight gain of rats taking aspirin, and significantly reduce the gastric tissue damage and gastric mucosal injury index. These data indicated that Codonopsis decoction may possess potential to reduce the side effects caused by oral aspirin and may help the safe use of aspirin.

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Antiulcerogenic and healing activity of hecogenin acetate in rodents

Sousa

Neto²,

Costa³

et al. 2022

Naunyn-Schmiedeberg's Arch Pharmacol

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Peptic ulcers are lesions in the gastric and duodenal mucosa generated by an imbalance between protective factors (gastroduodenal mucus secretion, bicarbonate production, adequate blood ow) and harmful factors (excess pepsin or hydrochloric acid). Some drugs used in peptic ulcer therapy are associated with adverse effects. The aim of this study was to evaluate the antiulcerogenic and healing activity of hecogenin acetate (HA) in acute and chronic models of gastric lesions in rodents. MethodsThe antiulcerogenic activity of HA was evaluated in models of gastric lesions induced by absolute ethanol and in acidi ed ethanol with HA (5; 10 and 20 mg/kg). For the model of gastric lesions induced by ischemia and reperfusion, rats were pre-treated with HA (5; 10, 20 mg/kg). After were submitted to 30 min of ischemia, followed by 1 h of reperfusion. To evaluate the healing activity was induced gastric ulcer using acetic acid (80%) in rats. After 24h they were treated for 7 consecutive days with HA (10 and 20 mg/kg). ResultsWere evaluated the possible signs of toxicity, measurement of the lesions, collagen deposition and histological analysis. HA signi cantly reduced the area of the lesion in models of gastric lesions induced by absolute and acidi ed ethanol, ischemia-induced gastric lesions and reperfusion, and regarding healing. In the collagen deposition, the presence and increase of collagen demonstrates healing effect. ConclusionThe AH has antiulcerogenic and healing potential demonstrated by the decrease in gastric injury and presence of collagen bers, respectively.

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Potent therapeutic effects of ruscogenin on gastric ulcer established by acetic acid

Cited by 28 publications

References 29 publications

Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

Codonopsis Decoction Inhibits Aspirin-Induced Gastric Mucosal Injury in Rats by Regulating COX, COX-1, COX-2, TNF-α, IL-6 and TGF-α

Antiulcerogenic and healing activity of hecogenin acetate in rodents

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