Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

Abstract

PurposeTo determine the potential protective and therapeutic effects and action mechanism of ruscogenin on cerulein-induced acute pancreatitis (AP) model in rats.MethodsOverall, 32 rats were attenuated to the sham (2-mL/kg/day isotonic solution for 4 weeks), control (20-µg/kg cerulein-induced AP for 12 hours), prophylaxis groups (cerulein-induced AP following 3-mL/kg/day ruscogenin for 4 weeks) and treatment (3-mL/kg/day ruscogenin following cerulein-induced AP for 12 hours). Blood samples were collected for biochemical analysis of nitric oxide synthase 1 (NOS1/neuronal NOS), malondialdehyde (MDA) and intercellular adhesion molecule 1 (ICAM-1). After sacrification, pancreas tissues were collected and prepared for light microscopic (hematoxylin and eosin), immunohistochemical (nuclear factor kappa B) and biochemical analysis (tumor necrosis factor-alpha [TNF-α], interleukin-6 and 1β [IL-6 and IL-1β], CRP, high-sensitivity CRP [hs-CRP] amylase, lipase, and ICAM-1). Ultrastructural analysis was performed by transmission electron microscopy.ResultsThe protective and therapeutic actions of ruscogenin were accomplished by improvements in histopathology, by decreasing blood cytokine levels of CRP, hs-CRP levels, TNF-α, IL-6, IL-1β, ICAM-1, by reducing the pancreatic enzymes amylase and lipase in blood, and by suppressing the expression of nuclear factor kappa B, ICAM-1, and NOS-1, but not MDA in pancreatic tissues. Ruscogenin also improved cerulein-induced ultrastructural degenerations in endocrine and exocrine cells, especially in treatment group.ConclusionThe present findings have demonstrated the beneficial protective and therapeutical effects of ruscogenin, nominating it as a highly promising supplementary agent to be considered in the treatment of AP, and even as a protective agent against the damages induced by disease.

Protective Effect of Nano-Moringa Oleifera leaves Extract and/or Low Doses of γ- Irradiation on Acute Pancreatitis Model Induced in Rats.

“…Local recruitment and stimulation of inflammatory cells (neutrophils and macrophages) released from injured acinar cells results in the generation of proinflammatory cytokines tumour necrosis factor-alpha in acute pancreatitis (TNF-). TNF-is released by activated pancreatic macrophages in response to local tissue injury, resulting in multiple organ failure [32] [37]. Moreover, Nieminen et al [38] discovered that CRP levels in serum were related with the severity of acute pancreatitis and its outcome.…”

Prophylactic low-molecular-weight heparin administration protected against severe acute pancreatitis partially by VEGF/Flt-1 signaling in a rat model

“…39 TNF-a and IL-6 are dominating pro-inflammatory cytokines in pancreatitis. 40 The present study was aimed to evaluate the protective effect and the underlying mechanism of prophylactic LMWH treatment on taurocholate-induced SAP in a rat model. It was found LMWH treatment resulted in reduction of the serum amylase activities at 1 h, 6 h, and 12 h and alleviation in pancreatic injury score and water content in rat SAP model.…”

Ruscogenin alleviates cognitive dysfunction by inhibiting the activation of isoflurane-induced NLRP3 inflammasome in aged mice

“…For example, ruscogenin exerted antifibrotic and anti-inflammatory effects to attenuate streptozotocin-induced diabetic nephropathy (Lu et al, 2014b). Monocrotaline-induced pulmonary hypertension (Bi et al, 2013), cerulein-induced acute pancreatitis (Ercan et al, 2019) and particulate matter-induced acute lung injury (Wang et al, 2021b) were ameliorated by ruscogenin through its anti-inflammatory capacity. Cerebral ischemic-induced inflammatory pathway was also suppressed by ruscogenin (Guan et al, 2013), and ruscogenin alleviated ischemia/reperfusion-induced blood-brain barrier dysfunction (Zhang et al, 2020).…”