Potent Reversible Inhibition of Myeloperoxidase by Aromatic Hydroxamates

Forbes, Louisa V.; Sjögren, Tove; Auchère, Françoise; Thong, Bob; Eriksson, Håkan; Unitt, John; Kettle, Anthony J.

doi:10.1016/j.freeradbiomed.2013.10.712

Cited by 11 publications

(22 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, the molecular docking calculations were validated using caffeic acid, gallic acid, quercetin, salicylhydroxamic acid (SHA) and 2‐(3,5‐bistrifluoromethylbenzylamino)‐6‐oxo‐1H‐pyrimidine‐5‐carbohydroxamic acid (HX1; a non‐covalent MPO inhibitor; PDB ID 4C1M) . Next, the docking calculations were performed with the following ligands: caffeic acid, trans ‐cinnamic acid, p ‐coumaric acid, ferulic acid, SHA and 4‐aminobenzoic acid hydrazide (ABAH), using the AutoDock Vina software with an exhaustiveness value of 1000.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Baccharis dracunculifoliaDC (Asteraceae) selectively modulates the effector functions of human neutrophils

Figueiredo-Rinhel

Melo

Bortot

et al. 2017

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

“…Eight 2.40‐GHz Intel processor cores were used for the simulations. The search volume corresponded to one heterodimer composed of a heavy and a light chain of the human MPO structure complexed with HX1 . The crystallographic waters inside the active site and HX1 were removed before the docking calculations.…”

Section: Methodsmentioning

confidence: 99%

Baccharis dracunculifoliaDC (Asteraceae) selectively modulates the effector functions of human neutrophils

Figueiredo-Rinhel

Melo

Bortot

et al. 2017

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

show abstract

“…Substrates gain access to the proximal surface of the cavern, wherein the heme prosthetic group acts as floor of the MPO active site. A narrower substrate-binding chase leads from the enzyme and can be seen in the MPO isoform C structure (see PDB accession number 1LXP), MPO cocrystallized with cyanide and thiocyanate (1DNW), the complex of MPO with cyanide (1DNU), the thioxanthine-inhibited MPO (3ZS1), the ceruloplasmin-bound MPO (4EJX), and the MPO with aromatic hydroxamates (4C1M) [32][33][34][35][36][37][38]. As mentioned, the protoporphyrin IX macrocycle of MPO has 3 covalent bonds with the parent protein as shown in Fig.…”

Section: What Sets Mpo Apart From Other Peroxidases?mentioning

confidence: 99%

Methods for measuring myeloperoxidase activity toward assessing inhibitor efficacy in living systems

Huang

Milton

Arnold

et al. 2016

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Myeloperoxidase aids in clearance of microbes by generation of peroxidase-mediated oxidants that kill leukocyte-engulfed pathogens. In this review, we will examine 1) strategies for in vitro evaluation of myeloperoxidase function and its inhibition, 2) ways to monitor generation of certain oxidant species during inflammation, and 3) how these methods can be used to approximate the total polymorphonuclear neutrophil chemotaxis following insult. Several optical imaging probes are designed to target reactive oxygen and nitrogen species during polymorphonuclear neutrophil inflammatory burst following injury. Here, we review the following 1) the broad effect of myeloperoxidase on normal physiology, 2) the difference between myeloperoxidase and other peroxidases, 3) the current optical probes available for use as surrogates for direct measures of myeloperoxidase-derived oxidants, and 4) the range of preclinical options for imaging myeloperoxidase accumulation at sites of inflammation in mice. We also stress the advantages and drawbacks of each of these methods, the pharmacokinetic considerations that may limit probe use to strictly cell cultures for some reactive oxygen and nitrogen species, rather than in vivo utility as indicators of myeloperoxidase function. Taken together, our review should shed light on the fundamental rational behind these techniques for measuring myeloperoxidase activity and polymorphonuclear neutrophil response after injury toward developing safe myeloperoxidase inhibitors as potential therapy for chronic obstructive pulmonary disease and rheumatoid arthritis.

show abstract

“…In all the cases, rhizome oil exhibited more potential than the leaf oil (p < 0.05). Suggestive evidences on 1,8-cineole-and monoterpene-rich EOs on reducing airway inflammation, MPO activity, and decreased cytokine levels portrait the importance and efficacy of this topical study for therapeutic development [65]. Held et al [28] have studied the oral anti-inflammatory effect of 1,8-cineole in various animal models, but the mechanism by which it exerts the activity was not clear.…”

Section: Inhibition Of Heat-induced Hemolysis By Aceosmentioning

confidence: 99%

Topical Anti‐Inflammatory Activity of Essential Oils of Alpinia calcarata Rosc., Its Main Constituents, and Possible Mechanism of Action

Chandrakanthan

Handunnetti

Premakumara

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

This study aimed at investigating the anti-inflammatory potential of essential oil from rhizome and leaf of Alpinia calcarata Rosc. (ACEO) with the focus of its topical anti-inflammatory activity along with its dominant compounds 1,8-cineole and α-terpineol using mouse ear edema model. ACEOs were analyzed by GC-MS. The anti-inflammatory activity was determined by studying the inhibition of overproduction of proinflammatory mediators—nitric oxide, reactive oxygen species, prostaglandins, cyclooxygenases, and cytokines induced by lipopolysaccharides in murine macrophages. Topical anti-inflammatory and antinociceptive activity was studied by 12-O-tetradecanoylphorbol-13-acetate (TPA) induced skin inflammation and formalin-induced pain model in mice, respectively. Rhizome oil has 1,8-cineole (31.08%), α-terpineol (10.31%), and fenchyl acetate (10.73%) as major compounds whereas the ACEO from leaves has 1,8-cineole (38.45%), a-terpineol (11.62%), and camphor (10%). ACEOs reduced the production of inflammatory mediators in vitro in a concentration-dependent manner. Further, ACEO and its major compounds reduced ear thickness, weight, myeloperoxidase, and cytokines significantly (p<0.01) in mouse ear. Dose-dependent reduction in flinching and licking in both the phases of pain sensation concludes the topical analgesic effect. Our findings suggest the potency of topical use of ACEOs for inflammatory disease conditions.

show abstract

Potent Reversible Inhibition of Myeloperoxidase by Aromatic Hydroxamates

Cited by 11 publications

References 47 publications

Baccharis dracunculifoliaDC (Asteraceae) selectively modulates the effector functions of human neutrophils

Baccharis dracunculifoliaDC (Asteraceae) selectively modulates the effector functions of human neutrophils

Methods for measuring myeloperoxidase activity toward assessing inhibitor efficacy in living systems

Topical Anti‐Inflammatory Activity of Essential Oils of Alpinia calcarata Rosc., Its Main Constituents, and Possible Mechanism of Action

Contact Info

Product

Resources

About