POTENT ANTIBACTERIAL OF GEL KAWANG FRUITS (Litocarpus celebicus, (Miq) Rehder) ETHANOL EXTRACT ORIGIN FROM PAPUA INDONESIA

Abstract

Objective: Kawang fruit (Lithocarpus celebicus (Miq.) Rehder) is native plant of Boven Digoel, Papua, Indonesia, have flavonoids which has antibacterial activity. Antiseptic is a substance used to inhibit the growth or destroy microorganisms that live on the surface of the body. Their resistance to antiseptics on the market and it needed a new antiseptic-based natural ingredients that are relatively safer. The purpose of this study to make an antiseptic gel with extract ethanol of Kawang fruit and determine its effectiveness against Staphylococcus aureus ATCC 29 213 and Escherichia coli ATCC 25922.Methods: Extraction, phytochemical screening, testing activities with the agar diffusion method, determination minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC), antiseptic gel formulation, and test preparation gel. The test results showed the Kawang fruit has activity against bacteria with value MIC and MBC on S. aureus ATCC 29213 is 5% (b/b) and 10% (b/b), and on E. coli ATCC 25922 is 10% and 20%. The extraction is formulated in the form of a gel with a concentration of 5%, 10%, and 20%. Formulations made using 2% hydroxy ethyl methyl cellulose. Results:The test results show the effectiveness of antiseptic gel extracts have inhibitory better on S. aureus ATCC 29213 than E. coli ATCC 25922. Conclusion:Antiseptic gel preparation of Kawang fruit ethanol extract at a concentration of 5% have shown their antibacterial effectivity. Its inhibition to S. aureus ATCC 29213 was better than E. coli ATCC 25 922.

Evaluation of Bactericidal Activity of an Antiseptic Gelthrough the Dilution-Neutralization Method

“…On the other hand, Gram-positive microorganisms present a greater sensibility to the product compared to the Gram-negative microorganisms such as Escherichia coli, with which a count greater than 300 cfu. ml -1 was obtained at 30 s, therefore the specification of the standard was considered and the result was reported as>3 x10 2 Some authors, [14][15][16] assure that the strains that present greater resistance in vitro to antimicrobial products are Escherichia coli and Klebsiella sp. However, considering the initial concentration of the inoculum of work for E. coli, a reduction of 99.9% was demonstrated for Escherichia coli from one minute of contact, results that are shown in table 5.…”