Potent Antibacterial Composite Nonwovens Functionalized with Bioactive Peptides and Polymers

Barbieri, Eduardo; Cutright, Camden C.; Ramesh, Srivatsan; Khan, Saad A.; Efimenko, Kirill; Genzer, Jan; Menegatti, Stefano

doi:10.1002/admi.202201061

Cited by 2 publications

(3 citation statements)

References 71 publications

(87 reference statements)

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“…This overnight stock was used to inoculate in triplicate 100 mL of LB in a 500 mL flask grown at 30 °C on a shaker at 225 rpm to log phase (OD > 1.0 approximately 9 h). Bacteria were harvested by centrifugation at 4100 rcf for 4 min and resuspended in sterile phosphate buffer saline (PBS) . The suspension was adjusted to 10 8 colony forming units (CFU)/mL, based on OD 600 measurement (BioRad Smartspec 3000, Hercules, CA) using an OD to CFU conversion of 10 8 CFU per 1.0 OD (600 nm), as determined by plate counting on LB agar.…”

Section: Materials and Methodsmentioning

confidence: 99%

Cellulose Acetate-Stabilized Pickering Emulsions: Preparation, Rheology, and Incorporation of Agricultural Active Ingredients

Sohail,

Pirzada,

Guenther

et al. 2023

ACS Sustainable Chem. Eng.

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We report the use of cellulose acetate (CA) nanoparticles (NPs) to produce oil in water Pickering emulsions. The CA NP can emulsify various oils and form stable emulsions at concentrations as low as 0.5 wt %. Rheological and microscopic analyses show evidence of interconnected NP aggregate networks between droplets. Yield stress measurements display evidence of "double" yielding. We postulate that the presence of the NP aggregates provides a secondary network between droplet clusters resulting in such behavior. We demonstrate the suitability of the emulsions as agriculture formulations by incorporating an agrochemical, abamectin (Abm), and a plant-growth-promoting microbe (PGPM) in the emulsions. Release assays exhibit sustained Abm release, promising higher efficacy at lower usage volumes. Incorporation of nonsporulating PGPM Pseudomonas simiae in the emulsions shows significantly higher microbe viability compared to controls after 70 days of storage. By demonstrating the application of CA NPs as a sustainable Pickering emulsifier, this study introduces the use of CA as a platform technology for the delivery of diverse agriculture cargos. A comprehensive evaluation of the system is articulated in a fundamental microstructure analysis and a demonstration of practical on-site attributes, including shelf-life stability and functional performance, verified through bioassays and plant growth studies.

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Section: Materials and Methodsmentioning

confidence: 99%

Cellulose Acetate-Stabilized Pickering Emulsions: Preparation, Rheology, and Incorporation of Agricultural Active Ingredients

Sohail,

Pirzada,

Guenther

et al. 2023

ACS Sustainable Chem. Eng.

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“…Using low surface energy polymeric binders such as PDMS in developing superhydrophobic coatings can improve the durability of antibacterial agents on coated surfaces. However, it can also potentially weaken the bactericidal performance of the used antibacterial agents due to preventing the direct contact between nanoparticles and bacteria . Therefore, for developing effective antibacterial superhydrophobic coatings on textiles, some parameters such as the concentration of binder, antibacterial agent type and content, thickness of coatings, and the possible impacts on the intrinsic characteristics of substrate should thoroughly be considered.…”

Section: Introductionmentioning

confidence: 99%

“…However, it can also potentially weaken the bactericidal performance of the used antibacterial agents due to preventing the direct contact between nanoparticles and bacteria. 33 Therefore, for developing effective antibacterial superhydrophobic coatings on textiles, some parameters such as the concentration of binder, antibacterial agent type and content, thickness of coatings, and the possible impacts on the intrinsic characteristics of substrate should thoroughly be considered. Achieving antibacterial superhydrophobic fabrics is challenging due to the uneven topography and geometry of fibers in the structure of a fabric, which can inevitably provide suitable sites for the adhesion of microorganisms and forming biofilms.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Superhydrophobic Cotton Fabric with Photothermal, Self-Cleaning, and Ultraviolet Protection Functionalities

Pakdel

Sharp

Kashi

et al. 2023

ACS Appl. Mater. Interfaces

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Cotton fabrics with superhydrophobic, antibacterial, UV protection, and photothermal properties were developed using Ag/PDMS coatings, and the role of coating formulations on the obtained functionalities was studied. Specific attention was paid to understanding the relationships between the fabrics’ superhydrophobicity and antibacterial activity against Escherichia coli (E. coli) bacteria. UV protection performance of Ag/PDMS coatings was thoroughly evaluated based on the variation of UV transmission rate through coated fabrics and photoinduced chemiluminescence spectra. Moreover, the effect of silver nanoparticles (Ag NPs) and PDMS on developing a photothermal effect on fabrics was discussed. It was found that the content of Ag NPs and PDMS played critical roles in determining the water contact angle (WCA) on modified fabrics. The largest WCA was 171.31°, which was durable even after numerous accelerated wash cycles and abrasions. Antibacterial activity of fabrics showed the positive effect of pure PDMS in bacterial growth inhibition. Moreover, it was found that the antibacterial performance was greatly affected by the content of Ag NPs loaded on fabrics rather than their superhydrophobic status. Moreover, increasing the content of Ag NPs boosted the UV protection level of fabrics, improved fabrics photostability, and reduced the UV transmission rate through fabrics. Testing the photothermal effect confirmed that the content of Ag NPs and PDMS both played prominent roles, where Ag acted as a photothermal agent and PDMS determined the NIR reflection rate from the coated surface. The modified fabrics were characterized using TGA, SEM, FTIR, and XRD techniques, and it was confirmed that using a higher amount of PDMS increased the amount of Ag NPs deposition on fabrics.

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Potent Antibacterial Composite Nonwovens Functionalized with Bioactive Peptides and Polymers

Abstract: or KRRWRIWLV-NH 2 at 0.5 mg mL −1 for 5 min. Following incubation, all wafers were copiously rinsed with MilliQ water and dried using a stream of N 2 .

Cited by 2 publications

References 71 publications

Cellulose Acetate-Stabilized Pickering Emulsions: Preparation, Rheology, and Incorporation of Agricultural Active Ingredients

Cellulose Acetate-Stabilized Pickering Emulsions: Preparation, Rheology, and Incorporation of Agricultural Active Ingredients

Antibacterial Superhydrophobic Cotton Fabric with Photothermal, Self-Cleaning, and Ultraviolet Protection Functionalities

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