Potency ranking of valproic acid analogues as to inhibition of cardiac differentiation of embryonic stem cells in comparison to their in vivo embryotoxicity

Jong, Esther de; Doedée, Anne; Reis-Fernandes, Marcos A.; Nau, Heinz; Piersma, Aldert H.

doi:10.1016/j.reprotox.2010.11.012

Cited by 28 publications

(15 citation statements)

References 34 publications

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“…In both human and mouse embryonic stem cells, VPA decreased the expression of nkx2.5 , a marker of cardiomyocyte differentiation (de Jong et al. ; Krug et al. ), although the mechanism underlying decreased expression of nkx2.5 is still unknown.…”

Section: Heartmentioning

confidence: 99%

“…VPA also induced cardiac malformation and pericardial edema in ZEBDET (Pruvot et al 2012;Inoue et al 2015). In both human and mouse embryonic stem cells, VPA decreased the expression of nkx2.5, a marker of cardiomyocyte differentiation (de Jong et al 2011b;Krug et al 2013), although the mechanism underlying decreased expression of nkx2.5 is still unknown. Diclofenac, a nonsteroidal anti-inflammatory drug, has been shown to cause cardiac malformation and pericardial edema in ZEBDET Inoue et al 2015).…”

Section: Heartmentioning

confidence: 99%

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Using zebrafish in systems toxicology for developmental toxicity testing

Nishimura

Inoue

Sasagawa

et al. 2016

Congenital Anomalies

165

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With the high cost and the long-term assessment of developmental toxicity testing in mammals, the vertebrate zebrafish has become a useful alternative model organism for high-throughput developmental toxicity testing. Zebrafish is also very favorable for the 3R perspective in toxicology; however, the methodologies used by research groups vary greatly, posing considerable challenges to integrative analysis. In this review, we discuss zebrafish developmental toxicity testing, focusing on the methods of chemical exposure, the assessment of morphological abnormalities, housing conditions and their effects on the production of healthy embryos, and future directions. Zebrafish as a systems toxicology model has the potential to elucidate developmental toxicity pathways, and to provide a sound basis for human health risk assessments.

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Section: Heartmentioning

confidence: 99%

Section: Heartmentioning

confidence: 99%

Using zebrafish in systems toxicology for developmental toxicity testing

Nishimura

Inoue

Sasagawa

et al. 2016

Congenital Anomalies

165

View full text Add to dashboard Cite

show abstract

“…For example a histopathology in testes observed in repeated-dose studies will be followed up by tests on spermatotoxicity models, not a two-generation study if the classification cannot be done based on the finding alone. This approach, which we termed "alert-driven testing" (Bremer et al, 2007a), bryotoxic potentials of even very closely related structures (de Jong et al, 2011). Optimizations of protocols were reported (De Smedt et al, 2008;Seiler et al, 2004;Seiler and Spielmann, 2011).…”

Section: Reduction To Key Eventsmentioning

confidence: 99%

A roadmap for the development of alternative (non-animal) methods for systemic toxicity testing

Basketter

Clewell

Kimber

et al. 2012

ALTEX

203

165

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“…This concept has been successfully applied for valproic acid, a known developmental toxic compound, and different structural analogues (87). Similarly, this concept might be applied in metal toxicity.…”

Section: Substance Tandemsmentioning

confidence: 99%

Compound selection for in vitro modeling of developmental neurotoxicity

Kadereit

Zimmer²,

Thriel³

et al. 2012

Front Biosci

100

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Development of in vitro systems, such as those based on embryonic stem cell differentiation, depends on the selection of adequate test and training compounds. We recommend the use of two classes of positive controls, the "gold standard compounds" for which developmental neurotoxicity (DNT) has been proven in man, and the "pathway compounds" that are known to disrupt signalling pathways and key processes relevant for neuronal differentiation. We introduce the concept of toxicity endophenotypes (TEP) as changes in neuronal connectivity resulting from exposure to developmental toxicants. Thus, TEPs provide the scientific rationale for modeling DNT with simple in vitro models of key neurodevelopmental events. In this context, we discuss scientific and technical aspects of the test compound selection process. We suggest to include compounds with unspecific toxicity, besides negative control compounds, and we recommend tandem approaches to determine relative toxicities instead of absolute measures. Finally, we discuss how to avoid pitfalls by distinguishing between unspecific forms of cytotoxicity and specific developmental neurotoxicity. A compilation of compound lists corresponding to the above-discussed principles supplement this review.

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Potency ranking of valproic acid analogues as to inhibition of cardiac differentiation of embryonic stem cells in comparison to their in vivo embryotoxicity

Cited by 28 publications

References 34 publications

Using zebrafish in systems toxicology for developmental toxicity testing

Using zebrafish in systems toxicology for developmental toxicity testing

A roadmap for the development of alternative (non-animal) methods for systemic toxicity testing

Compound selection for in vitro modeling of developmental neurotoxicity

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