Potency of jellyfish alkaloid (Aeginura sp) on kidney histopathology and relative percent survival (RPS) of tiger grouper (Epinephelus fuscoguttatus) against Vibrio harveyi

Abstract

The purpose of this research were to determine the effect of alkaloid jellyfish compounds on kidney histopathology and to know the best doses to the Relative Percent Survival (RPS) of tiger grouper. The method of this research was descriptive and treatment with Completely randomized design. The treatment of active alkaloid compound on feed was given for 28 days. And then challenge-tested to 107 cells.ml−1 of Vibrio harveyi into the media for one day. Using alkaloids compound of Aeginura sp. on the feed with the doses of Control= 0 g alkaloid / kg feed; A = 0.5 g alkaloid / kg feed; B = 0.75 g alkaloid / kg feed; C = 1.0 g alkaloid / kg feed; Alkaloid D = 1.25 g / kg feed. The result of the scoring shows that the 4 treatment with given alkaloid dosage are able to decrease the damage level on the tissues of the fish’ kidneys. The average total damage of treatment C =1gr Alkaloid dosage is found to have the lowest value compared to another treatment with the percentage damages of 13,47% which is categorized low. Those results show better value than the infected fish without any medication with total damage of 42.27% within the medium category. The best RPS was found at a treatment of C with the value of 100%. For further study, it was suggested using a dose of 1 g alkaloid / kg feed for the best result. It was proved by an absent of the damage on kidney and the value of RPS reached 100%.

Effect of the Exposure of Aloe Vera Polyphenol Fraction on Changes in Blood Glucose of Koi Fish (Cyprinus carpio) as a Stress Response

“…The observational data on clinical symptoms below show that polyphenols are substances that can make fish unstable, can cause damage to internal organs, pale and red spots on the operculum causing the number of erythrocytes, hematocrit, and hemoglobin to decrease, this toxic polyphenol is a bioactive compound that is cytotoxic. This poison can disrupt or damage cell membranes, which can interfere with the transport of compounds in and out of cells causing rupture of blood vessels resulting in injury and hematopoietic organs that cannot compensate for the loss of erythrocytes due to bleeding [39,40]. The toxic mechanism of a compound, for example polyphenols, will work by totally damaging the cytoplasmic membrane by precipitating cell proteins, Ah receptors (AhR) that can bind to ligands such as polyphenolic compounds, polycyclic aromatic HKs or dioxins [41].…”