Potency of double-layered Poly L-lactic Acid scaffold in tissue engineering of tendon tissue

Inui, Atsuyuki; Kokubu, Tatsuo; Makino, Takeshi; Nagura, Issei; Toyokawa, Narikazu; Sakata, Ryosuke; Kotera, Masaru; Nishino, Takashi; Fujioka, Hiroyuki; Kurosaka, Masahiro

doi:10.1007/s00264-009-0917-8

Cited by 35 publications

(32 citation statements)

References 28 publications

(32 reference statements)

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“…Then, PLLA is modified or copolymerized with other degradable polymers to reduce degradation time, as shown by the use of radiations to create radicals in the ester alpha carbon which, upon rearrangement, shortens the polymer backbone through the removal of an ester bond and the release of carbon dioxide [100,101]. PLLA is used as bone fixator, scaffold for bone [102,103], cartilage [104], tendon [105], neural [106], and vascular [107] regeneration. Similarly, PDLLA is an amorphous polymer with the random positions of its two isomeric monomers within the polymer chain.…”

Section: Polyestersmentioning

confidence: 99%

Biodegradable polymers for dental tissue engineering and regeneration

Conte¹,

Riccitiello²,

Luise³

et al. 2017

Biodegradable Polymers: Recent Developments and New Perspectives

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Section: Polyestersmentioning

confidence: 99%

Biodegradable polymers for dental tissue engineering and regeneration

Conte¹,

Riccitiello²,

Luise³

et al. 2017

Biodegradable Polymers: Recent Developments and New Perspectives

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“…Clinical studies have shown that a local inflammatory reaction to PLLA might arise because its degradation products are acidic [9] and that the slow degradation rate of PLLA fibres might inhibit host tissue maturation [10]. Therefore, we hypothesised that fewer synthetic fibres would reduce the inflammatory reaction and accelerate host tissue maturation, resulting in better ligament reconstruction.…”

Section: Introductionmentioning

confidence: 99%

“…However, absorbable scaffolds can cause adverse effects, including local inflammatory reactions [9,10].…”

Section: Introductionmentioning

confidence: 99%

Ligament regeneration using an absorbable stent-shaped poly-l-lactic acid scaffold in a rabbit model

Nishimoto

Kokubu

Inui

et al. 2012

International Orthopaedics (SICOT)

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Purpose Ligaments are frequently damaged in sports activities and trauma, and severe ligament injury can lead to joint instability and osteoarthritis. In this study, we aimed to regenerate the medial collateral ligament (MCL) using an absorbable stent-shaped poly-L-lactic acid (PLLA) scaffold in a rabbit model to examine the biocompatibility and mechanical properties. Methods Twenty-three Japanese white rabbits were used in this study. MCL defects were surgically created in the knee joints and then reconstructed using stent-shaped PLLA scaffolds. As controls, flexor digitorum longus (FDL) tendons were implanted into the contralateral knees. Seven rabbits were sacrificed at three time points, conducted four, eight and 16 weeks after the operation. The regenerated tissues were histologically evaluated using fibre alignment scoring, morphology of fibroblast scoring and immunohistochemical analysis of types I and III collagen. The regenerated tissues were also biomechanically evaluated by measuring the ultimate failure load and stiffness. Results At four weeks post-operation, spindle-shaped cells were observed on the inside of the scaffolds. At eight weeks, maturation of the regenerated tissues and collagen fibre alignment parallel to the ligaments was observed. At 16 weeks, the fibre alignment had become denser. The fibre alignment and morphology of fibroblast scores significantly increased in a time-dependent manner. Expression of type I collagen was more strongly observed in the scaffold group at eight and 16 weeks post-operation than at four weeks. Type III collagen was also observed at four, eight and 16 weeks post-operation. A thin layer of fibrocartilage was observed at the ligament-bone junction at eight and 16 weeks. The ultimate failure load of the scaffold group was 46.7±20.7 N, 66.5±11.0 N and 74.3±11.5 N at four, eight and 16 weeks post-operation, respectively. There was no statistical difference between the normal MCL and the scaffold group at 16 weeks post-operation. Conclusions The stent-shaped PLLA scaffold allowed for MCL regeneration with type I collagen expression and fibrocartilage formation and resulted in sufficient mechanical function.

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“…Lastly, the biomaterial should support meniscocyte growth and differentiation. Different types of biomaterials made from natural polymers such as collagen, or synthetic polymers including polyglycolide, polylactides [13] and polycaprolactone [14] have been developed. Particularly good results were seen with BF-1 CO-PET, which is a biomaterial consisting of fast degrading hyaluronic acid (30 %) and slower degrading poly-ε-caprolactone (70 %) augmented with polyethyleneteraphtalate (PET) fibers [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…The addition of PET is usually done to augment the biomechanical capabilities of the scaffold [12,13], yet recent research has shown a beneficial effect of PET on the differentiation of cells of the mesenchymal lineage other than meniscocytes. If such effects also existed for mensicocytes they might have a profound effect on in vivo results of meniscus repair with a PET augmented biomaterial [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Polyethylene terephthalate (PET) enhances chondrogenic differentiation of ovine meniscocytes in a hyaluronic acid/polycaprolactone scaffold in vitro

Koller

Nehrer

Vavken

et al. 2012

International Orthopaedics (SICOT)

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Purpose The purpose of this study was to assess the effect of polyethylene terephthalate (PET) on proliferation, differentiation, and attachment of ovine meniscocytes seeded in a hyaluronic acid/polycaprolactone biomaterial (BF-1) Methods BF-1 (30 % hyaluronic acid and 70 % polycaprolactone) cylinders with PET (CO-PET) or without PET, were seeded with 2x10 6 ovine meniscus cells. The specimens were harvested in triplets at 12 hours, seven, 14, 21 and 28 days. DNA content was measured to test proliferation, histological analysis for cell morphology, and biochemical assessment of glycosaminoglycan content and RT-PCR for type I and II collagen were used to assess differentiation, with immunohistochemistry as post-translational control. Attachment was evaluated by electronic microscopy at 28 days. Results DNA content was consistent and equal across groups, suggesting no effect of PET on cell proliferation. However, the BF-1 CO-PET showed a higher percentage of cells with spherical morphology which is typical for a chondrocytic phenotype. This biomaterial with PET also showed a higher type II collagen mRNA expression and an eightfold higher GAG-content than the material without PET. Small amounts of type I collagen mRNA expression were present on both materials at all time points. PCR results were confirmed by immunohistochemistry. Conclusion Addition of PET to a hyaluronic acid/polycaprolactone biomaterial enhances a cartilaginous phenotype, increased type II collagen mRNA expression and a higher GAG production in ovine mensicocytes.

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Potency of double-layered Poly L-lactic Acid scaffold in tissue engineering of tendon tissue

Cited by 35 publications

References 28 publications

Biodegradable polymers for dental tissue engineering and regeneration

Biodegradable polymers for dental tissue engineering and regeneration

Ligament regeneration using an absorbable stent-shaped poly-l-lactic acid scaffold in a rabbit model

Polyethylene terephthalate (PET) enhances chondrogenic differentiation of ovine meniscocytes in a hyaluronic acid/polycaprolactone scaffold in vitro

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