Potato virus Y infection affects flavonoid profiles of Physalis angulata L.

Abstract

Physalis angulata L. is an annual herb of Solanaceae. Among several biological activities described for this species, its cytotoxic action is due to the presence of flavonoids. Natural infection of P. angulata by Potato virus Y, strain O (PVY O ), has already been described. The main goal of this study is to verify whether PVY O infection influences the secondary metabolism of P. angulata, evaluating total amount of phenolic compounds and the content and profiles of flavonoids. Twenty-day-old plants were distributed into three groups: control (C 1 ), mechanically injured (C 2 ), and artificially inoculated (PVY O ). After 21 days, leaves from local and systemic infection were collected and compounds were analyzed by UV-Vis and HPLC. Five flavonoids were partially identified as rutin, kaempferol-4 0 -O-ramnoglucosyl, kaempferol-3-Oramnoglucosyl, and two other kaempferol derivatives. Quantitative analysis of total phenolic compounds and total flavonoid in the local infection were similar, with higher percentage in the C 1 group, followed by the PVY O group and, consequently, the lowest percentage present in C 2 group. There were no qualitative differences in flavonoid profiles between healthy and infected groups. However, lower concentrations of these compounds were detected in the systemic infection of infected plants, suggesting that PVY O influences secondary metabolism by reducing the amounts of flavonoids.

Ocimum basilicum-Mediated Synthesis of Silver Nanoparticles Induces Innate Immune Responses against Cucumber Mosaic Virus in Squash

“…The obtained results indicated a reduction in both phenolic and flavonoid contents (42.8 ± 1.5 and 11.5 ± 0.8 mg/g d. wt, respectively) in CMV treatment compared to Mock plants (76.7 ± 9.1 and 13 ± 1.2 mg/g d. wt, respectively) which is in line with the free radical scavenging activity ( Figure 7 B,C). The reduction of phenolic and flavonoid contents was reported among the main traits for several viral plant infections [ 73 , 74 ] and could be attributed to viral suppressor activity and severe mosaic symptoms [ 75 ]. The treatment with Ag-NPs revealed parallel impacts on phenolic and flavonoid contents, where the pre-CMV treatment significantly enhanced both contents to 72.1 ± 9.7 and 13.9 ± 2.1 mg/g d. wt.…”