Potato Cold Acclimation

Chen, Hwei-Hwang; Li, P.H.

doi:10.1016/b978-0-12-447602-8.50007-6

Cited by 21 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the levels of expression of the genes corresponding to pSM784 in cultivars Iroquois, Algonquin, and Trek are, respectively, 33%, 24%, and 10% of that in Anik. A similar trend is shown by the expression ofthe genes corresponding to pSM2358 and pSM220 1. The level of expression in nonacclimated seedlings was similar in all cultivars and was only 3 to 4% for pSM784, …”

Section: Cas-gene Expression Related To Freezing-tolerancesupporting

confidence: 55%

“…ABA was used because it has been implicated in plant responses to environmental stresses (14), particularly to water stress ( 14) and low temperature stress ( 1,3). Water stress was imposed by placing the seedlings in polyethylene glycol-6000 (water potential of -15 bars), heat 4Abbreviations: LT5o, temperature at which 50% seedlings fail to survive; CAS, cold-acclimation-specific; ds, double-stranded; kb, kilobase; ss, single-stranded; SSC, 150 mm NaCl-1 5 mm sodium citrate.…”

Section: Administration Of Stressmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Cloning and Relationship to Freezing Tolerance of Cold-Acclimation-Specific Genes of Alfalfa

Mohapatra¹,

Wolfraim²,

Poole³

et al. 1989

Plant Physiol.

157

125

View full text Add to dashboard Cite

Cold-acclimation-specific (CAS) gene expression has been examined by screening a cDNA library prepared from poly(A)+ RNA of cold-acclimated seedlings of a freezing-tolerant variety of alfalfa (Medcago falcata cv Anik). Three CAS cDNA clones, pSM784, pSM2201, and pSM2358, representing different sequence species, have been used to investigate the relative abundance and time-course of accumulation of corresponding transcripts. Results obtained show that the expression of these CAS genes is regulated in a coordinated manner most likely at the level of transcription. The expression of genes, as measured by mRNA abundance corresponding to the three CAS cDNA clones, is not stimulated or induced by heat shock, water stress, abscisic acid, or wounding. A positive correlation is observed between the expression of these cloned sequences and the degree of freezing-tolerance in four alfalfa cultivars.Freezing temperatures constitute one ofthe most important environmental constraints limiting the productivity and distribution of plants. Although plants are known to differ in their ability to withstand freezing temperatures, the molecular/genetic basis of this differential freezing-tolerance is unclear. It is known, however, that a prior exposure of plants to low nonfreezing temperatures (cold-acclimation) increases their tolerance to subsequent freezing (14). Many physiological and biochemical changes are known to occur in plants during cold-acclimation (5, 10, 13, 21) and it has been suggested (26) Cold-acclimation at 4°C was carried out as described (17) for the time periods mentioned in the text or figure legends. Tests of freezing tolerance were also carried out as described previously (17) except that several temperatures, namely, -50C, -8C, -12C, and -15C were used and LT50 values4 were determined. Administration of StressSeedlings were subjected to water stress, heat shock, wounding, and ABA treatment. ABA was used because it has been implicated in plant responses to environmental stresses (14), particularly to water stress ( 14) and low temperature stress ( 1,3). Water stress was imposed by placing the seedlings in polyethylene glycol-6000 (water potential of -15 bars), heat 4Abbreviations: LT5o, temperature at which 50% seedlings fail to

show abstract

Section: Cas-gene Expression Related To Freezing-tolerancesupporting

confidence: 55%

Section: Administration Of Stressmentioning

confidence: 99%

Molecular Cloning and Relationship to Freezing Tolerance of Cold-Acclimation-Specific Genes of Alfalfa

Mohapatra¹,

Wolfraim²,

Poole³

et al. 1989

Plant Physiol.

157

125

View full text Add to dashboard Cite

show abstract

“…These plants were subjected to somewhat reduced light intensity during cold acclimation as compared to control plants; however, the daylength was not changed (Fowler and Thomashow 2002). These results may have limited applicability to over-wintering woody species that respond to a combination of shortening daylength and sub-freezing temperatures through fall and winter and acquire maximal cold acclimation over a period of months (Weiser 1970;Sakai and Larcher 1987) as opposed to a few hours or few days as in herbaceous species (Chen and Li 1982;Ristic and Ashworth 1993).…”

Section: Introductionmentioning

confidence: 86%

Comparative analysis of expressed sequence tags from cold-acclimated and non-acclimated leaves of Rhododendron catawbiense Michx

Hui

Dhanaraj

Rowland³

et al. 2005

Planta

109

View full text Add to dashboard Cite

An expressed sequence tag (EST) analysis approach was undertaken to identify major genes involved in cold acclimation of Rhododendron, a broad-leaf, woody evergreen species. Two cDNA libraries were constructed, one from winter-collected (cold-acclimated, CA; leaf freezing tolerance -53 degrees C) leaves, and the other from summer-collected (non-acclimated, NA; leaf freezing tolerance -7 degrees C) leaves of field-grown Rhododendron catawbiense plants. A total of 862 5'-end high-quality ESTs were generated by sequencing cDNA clones from the two libraries (423 from CA and 439 from NA library). Only about 6.3% of assembled unique transcripts were shared between the libraries, suggesting remarkable differences in gene expression between CA and NA leaves. Analysis of the relative frequency at which specific cDNAs were picked from each library indicated that four genes or gene families were highly abundant in the CA library including early light-induced proteins (ELIP), dehydrins/late embryogenesis abundant proteins (LEA), cytochrome P450, and beta-amylase. Similarly, seven genes or gene families were highly abundant in the NA library and included chlorophyll a/b-binding protein, NADH dehydrogenase subunit I, plastidic aldolase, and serine:glyoxylate aminotransferase, among others. Northern blot analyses for seven selected abundant genes confirmed their preferential expression in either CA or NA leaf tissues. Our results suggest that osmotic regulation, desiccation tolerance, photoinhibition tolerance, and photosynthesis adjustment are some of the key components of cold adaptation in Rhododendron.

show abstract

“…Cellular and metabolic changes during cold acclimation include a rise in the levels of sugars, soluble proteins, proline, and organic acids, as well as the appearance of new isoforms of proteins and an altered lipid membrane composition (Chen and Li, 1982;Hughes and Dunn, 1996;Chen and Li, 2005). Steponkus (1984) suggested that the accumulation of compatible solutes in the cytoplasm contributes to freezing survival by reducing the rate and extent of cellular dehydration, by sequestering toxic ions and/or by protecting macromolecules against dehydration-induced denaturation.…”

Section: Discussionmentioning

confidence: 97%

Effects of cold-hardening on compatible solutes and antioxidant enzyme activities related to freezing tolerance in Ammopiptanthus mongolicus seedlings

et al. 2008

View full text Add to dashboard Cite

Cold acclimation is associated with many metabolic changes that lead to an increase of freezing tolerance. In order to investigate the biochemical process of cold acclimation in Ammopiptanthus mongolicus, seedlings were acclimated at 2°C under 16-h photoperiod (150 μmol·m -2 ·s -1 photosynthetically active radiation) for 14 d. Freezing tolerance in seedlings increased after 14 d of cold-hardening. Contents of protein, proline and solute carbohydrate in cotyledon increased after cold acclimation. Patterns of isozymes of superoxide dismutase (SOD), peroxidase, catalase and polyphenol oxidase (PPO) were investigated. The activities of SOD, peroxidase and PPO in cold acclimated plants were increased during cold-hardening. We deduced that compatible solutes and antioxidant enzymes play important roles in development of freezing tolerance during cold acclimation in this evergreen woody plant.

show abstract

Potato Cold Acclimation

Cited by 21 publications

References 29 publications

Molecular Cloning and Relationship to Freezing Tolerance of Cold-Acclimation-Specific Genes of Alfalfa

Molecular Cloning and Relationship to Freezing Tolerance of Cold-Acclimation-Specific Genes of Alfalfa

Comparative analysis of expressed sequence tags from cold-acclimated and non-acclimated leaves of Rhododendron catawbiense Michx

Effects of cold-hardening on compatible solutes and antioxidant enzyme activities related to freezing tolerance in Ammopiptanthus mongolicus seedlings

Contact Info

Product

Resources

About