Potassium-Selective Atomic Force Microscopy on Ion-Releasing Substrates and Living Cells

Schär-Zammaretti, Prisca; Ziegler, Urs; Forster, Ian C.; Groscurth, Peter; Spichiger‐Keller, Ursula E.

doi:10.1021/ac025605n

Cited by 21 publications

(12 citation statements)

References 45 publications

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“…These areas of high concentration were used as indicators of the presence of active potassium channels. Relative regional ion channel activity can then be tracked by tracking this adhesion between different curves over time [49]. Such measurements have not yet been performed on neurons; however, similar imaging techniques might be employed to track signaling activity in axons.…”

Section: Future Directionsmentioning

confidence: 99%

Neuron Biomechanics Probed by Atomic Force Microscopy

Spedden

Staii

2013

IJMS

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Mechanical interactions play a key role in many processes associated with neuronal growth and development. Over the last few years there has been significant progress in our understanding of the role played by the substrate stiffness in neuronal growth, of the cell-substrate adhesion forces, of the generation of traction forces during axonal elongation, and of the relationships between the neuron soma elastic properties and its health. The particular capabilities of the Atomic Force Microscope (AFM), such as high spatial resolution, high degree of control over the magnitude and orientation of the applied forces, minimal sample damage, and the ability to image and interact with cells in physiologically relevant conditions make this technique particularly suitable for measuring mechanical properties of living neuronal cells. This article reviews recent advances on using the AFM for studying neuronal biomechanics, provides an overview about the state-of-the-art measurements, and suggests directions for future applications.

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Section: Future Directionsmentioning

confidence: 99%

Neuron Biomechanics Probed by Atomic Force Microscopy

Spedden

Staii

2013

IJMS

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“…Researchers are attempting to use the former method to detect cellular metabolites directly with sensors and thus detect cell growth [2]. Analyses of various substances are being tested using the latter method [3,4], which has been used to measure inorganic ions, glucose and other substances inside cells.…”

Section: Mammalian Cell Sensorsmentioning

confidence: 99%

Live Cell-Based Sensor Devices

Okuda-Shimazaki¹,

Wada²,

Taniguchi³

2007

TOBIOTJ

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Living cells maintain life functions by responding quickly and with great sensitivity to changes in the external environment. Consequently, sensors using cells as active elements are thought to be able to perform analyses faster and with more sensitivity than previously possible. Cell-based sensors can be roughly divided into two types. The first uses microorganisms such as Escherichia coli or yeast as active elements (Microbial cells). The second type uses human and animal cells (Mammalian cells). The first type can be cultivated rather easily and has the advantages of being inexpensive and portable. The second type is more complex but has the advantage of potentially being used with human subjects. Most research in this area is concentrated on the first type, microbial sensors, but research on sensors that use mammalian cells has recently become more widespread.We are exploring the modification of mammalian cells using genetic engineering techniques and developing nextgeneration cell sensors that can visually represent specific reactions. Recently, few researchers, including our group, have successfully produced devices from sensor cells that can process a variety of specimens using MEMS, NEMS, and other nano/micro processing technologies. In this review, we will introduce our research and describe recent trends in this field.

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“…The ultimate goal of the project was to support drug development and to show the feasibility of localizing potassium channels on living tissues and cell membranes with chemically modified AFM-cantilevers [37]. In addition, with AFM the mapping of the topography may be combined with the mapping of ion channel activity.…”

Section: Application Of the Model To Bk Ca Channels Of Living Mdck-f1mentioning

confidence: 99%

“…AFM experiments and results of force volume files elaborated as adhesion force maps were compared to immunofluorescence images, to Patch-Clamp electrophysiology experiments and to scans of the topography [14] [37]. Ion-channels of living cells were investigated at the Institute of Anatomy at the University of Zürich (Prof. Dr. med.…”

Section: Application Of the Model To Bk Ca Channels Of Living Mdck-f1mentioning

confidence: 99%

The Centre for Chemical Sensors and Chemical Information Technology (CCS) in Micro- and Nanotechnology

Spichiger‐Keller¹,

Schär-Zammarettia²

2004

Chimia

Self Cite

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A selection of the most challenging projects in micro-and nanotechnology tackled at CCS during the last few years are discussed. The ultimate challenge was to localize voltage-gated and ligand-gated ion channels on membranes of living cells by ion-selective AFM-tips. In order to address this goal, a procedure to simulate the ion transport over ion-channels was developed. Two types of living cells were investigated; Madin-Darby canine kidney (MDCK-F) cells and adult rat cardiomyocytes (ARC). The results of force microscopy were compared to the patch clamp technique and to immunoselective staining and localization by confocal laser scanning microscopy (CLSM). The lateral resolution of the AFM imaging technique, the rate-limiting steps, the force applied to the cells by the AFM-cantilever and the mechanism of the detection principle are addressed.

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Potassium-Selective Atomic Force Microscopy on Ion-Releasing Substrates and Living Cells

Cited by 21 publications

References 45 publications

Neuron Biomechanics Probed by Atomic Force Microscopy

Neuron Biomechanics Probed by Atomic Force Microscopy

Live Cell-Based Sensor Devices

The Centre for Chemical Sensors and Chemical Information Technology (CCS) in Micro- and Nanotechnology

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