Chloroplast DNA restriction-site variation was surveyed among 40 accessions representing all 11 species of giant senecios (Dendrosenecio, Asteraceae) at all but one known location, plus three outgroup species. Remarkably little variation (only 9 variable sites out of roughly 1000 sites examined) was found among the 40 giant senecio accessions, yet as a group they differ significantly (at 18 sites) from Cineraria deltoidea, the closest known relative. This pattern indicates that the giant senecios underwent a recent dramatic radiation in eastern Africa and evolved from a relatively isolated lineage within the Senecioneae. Biogeographic interpretation of the molecular phylogeny suggests that the giant senecios originated high on Mt. Kilimanjaro, with subsequent dispersion to the Aberdares, Mt. Kenya, and the Cherangani Hills, followed by dispersion westward to the Ruwenzori Mountains, and then south to the Virunga Mountains, Mt. Kahuzi, and Mt. Muhi, but with dispersion back to Mt. Elgon. Geographic radiation was an important antecedent to the diversification in eastern Africa, which primarily involved repeated altitudinal radiation, both up and down the mountains, leading to morphological parallelism in both directions. In general, the plants on a given mountain are more closely related to each other than they are to plants on other mountains, and plants on nearby mountains are more closely related to each other than they are to plants on more distant mountains. The individual steps of the geographic radiation have occurred at various altitudes, some clearly the result of intermountain dispersal. The molecular evidence suggests that two species are extant ancestors to other species on the same or nearby mountains.The giant senecios are an unusual group of plants that have diversified to occupy a range of habitats, from 2500 to 4600 m, on 10 mountains >3300 m tall within 40 of the equator in Zaire, Rwanda, Uganda, Kenya, and Tanzania (1). Now segregated as the genus Dendrosenecio (Hauman ex Hedberg) Nordenstam, the giant senecios comprise 11 species and 6 nonautonymic subspecific taxa (2). Their habitats range from upper montane forest (mist-forest) through the giant heather zone and highaltitude wet meadows and extend almost to the upper limit of the unusual afro-alpine zone, whose climate has been characterized as "summer every day and winter every night" because strong diurnal fluctuations exceed seasonal variation (3). These plants typically have a giant-rosette growth form with a massive terminal leaf rosette borne atop a thick woody stem. During reproduction, a large terminal inflorescence is produced, and two to four lateral branches are usually initiated. Old plants appear as candelabras the size of telephone poles, with each branch bearing a leaf rosette (Fig. 1). The giantrosette growth form, large pith volume, marcescent foliage, and nyctinasty have evolved independently in the montane east The giant senecios show an unusual trend of massive plants occurring at high elevations, with smaller plan...