Postsynthetic Modification of the Magnetic Zirconium–Organic Framework for Efficient and Rapid Solid-Phase Extraction of DNA

Abstract

In recent years, several approaches have been applied to modify metal–organic frameworks (MOFs) owing to their excellent structural tunability such as higher extraction efficiency than that of primitive crystals. Herein, Zr-based MOFs (UiO-66-NH2) with a suitable size modulated by acetic acid were successfully synthesized for effective DNA extraction. The bonding conformations and adsorption mechanism indicated a high affinity between UiO-66-NH2 and the DNA molecules. Furthermore, Fe3O4 nanoparticles were immobilized on the UiO-66-NH2 surface to allow MOFs with magnetism. The magnetic zirconium–organic framework (MZMOF) retained the intact structure of MOFs and simplified subsequent extraction operations. In the DNA recovery investigation, MZMOF showed high recovery efficiency for both short-stranded DNA (90.4%) and pseudovirus DNA (95.1%). In addition, it showed superior DNA extraction efficiency from plasma (57.6%) and swab preservation solution (86.5%). The prepared MZMOF was employed for highly specific extraction of viral DNA and cfDNA from samples. To further simplify the extraction process, MZMOF was applied to immiscible phase filtration assisted by a surface tension (IFAST) chip for facilitating rapid DNA extraction with sensitive point-of-care testing. The developed MZMOF-based extraction method has significant potential for increasing the demand for rapid and efficient nucleic acid extraction.

Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

“…Nucleic acid extraction has been miniaturized to require only microliter or milliliter volumes to reduce the use of toxic organic solvents [ 32 , 33 ], but it is still difficult to be compatible with microfluidic devices for reasons such as corrosiveness. Instead, solid-phase nucleic acid extraction methods, which allow for the separation of substances with limited amounts of reagents, are more frequently used as assays paired with microfluidic chips [ [34] , [35] , [36] ]. Therefore, we focus on the latest advances in the solid phase extraction field.…”

Metal‐Organic Frameworks Nanocarriers for Functional Nucleic Acid Delivery in Biomedical Applications

“…Pan et al. prepared magnetic zirconium‐Organic Framework (MZMOF) for DNA extraction [82] . Correspondingly, Meng et al.…”

“…Pan et al prepared magnetic zirconium-Organic Framework (MZMOF) for DNA extraction. [82] Correspondingly, Meng et al prepared a nano-MOFs complex which dispersed in a lactic acid-based deep eutectic solvent(Fe 3 O 4 -COOH@UiO- [74] Copyright (2013), with permission from Publisher.…”

UV-Assisted Hyperbranched Poly(β-amino ester) Modification of a Silica Membrane for Two-Step Microfluidic DNA Extraction from Blood

“…However, proteins in the lysed cell can nonspecifically bind to these surfaces and can be removed through selective washing with cleaving enzymes, while ethanol can maintain the bound DNA to the surface. When the pH is increased, the negative charge density on the membrane surface results in greater electrostatic repulsion between the DNA and the silica surface, causing elution. − The wide adaptation of silica surfaces is due to its stability, biocompatibility, and easy modification properties in solid-phase extraction. However, a common problem is the carry-over of residual chaotropic salts and ethanol into the amplification reaction, which can inhibit polymerase amplification …”