Postprandial recruitment of neutrophils may contribute to endothelial dysfunction

Oostrom, A.J.H.H.M. van; Sijmonsma, Tjeerd P.; Verseyden, C.; Jansen, Eugène; Koning, Eelco J.P. de; Rabelink, Ton J.; Cabezas, Manuel Castro

doi:10.1194/jlr.m200419-jlr200

Cited by 226 publications

(205 citation statements)

References 53 publications

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“…Consistent with previous studies [37][38][39], we found that plasmatic triacylglycerols and free fatty acids increased in plasma after a high-fat challenge in healthy men, and peaked 4 h after fat consumption (Fig. 1).…”

Section: Discussionsupporting

confidence: 81%

Modulation of gene expression in endothelial cells by hyperlipaemic postprandial serum from healthy volunteers

et al. 2010

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A single high-fat challenge induces plasmatic pro-inflammatory and pro-oxidative responses in the postprandial state, even in healthy men. This period is also associated with vascular endothelial dysfunction, which is an early event in the development of cardiovascular diseases. However, knowledge about the mechanisms involved in postprandial hyperlipaemia-induced endothelial dysfunction is sparse. An objective of our study was to characterize the behaviour and gene expression of vascular endothelial cells exposed to postprandial hyperlipaemic sera. Human umbilical vein endothelial cells (HUVECs) were cultured in media containing 10% serum from healthy men withdrawn either before or 4 h after a high-fat challenge. Endothelial cell proliferation, adhesion and migration were then assessed. The transcriptomic profiles of endothelial cells exposed to pre and postprandial sera were also compared. Exposure to postprandial hyperlipaemic sera significantly decreased HUVEC proliferation when compared to preprandial serum (P \ 0.0001), while no changes in migration or endothelial/monocyte interactions were observed. The transcriptomic analysis revealed changes in the expression of 675 genes, of which 431 have a known function. Among them, a set of differentially expressed genes was linked to cell cycle regulation and apoptosis and are regulated in favour of cell cycle arrest or death. This result was confirmed by measuring the induction of apoptosis after postprandial sera exposure (P = 0.011). Taken together, the transcriptomic results and pathway analysis showed that postprandial serum promotes apoptosis in HUVECs, potentially through the activation of the p53 network. We conclude that upon postprandial serum exposure, vascular endothelial cells transcriptionally regulate genes involved in the control of cell cycle and death to favour growth arrest and apoptosis. These findings support the hypothesis that postprandial hyperlipaemia is associated with vascular dysfunction and offer new insights into the mechanisms involved.

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Section: Discussionsupporting

confidence: 81%

Modulation of gene expression in endothelial cells by hyperlipaemic postprandial serum from healthy volunteers

et al. 2010

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“…Il-8 concentration is increased after an oral glucose tolerance test in nondiabetic subjects [31]. WBCs increase after a meal [32], or glucose challenge with a significant increase of the plasma IL-8 level [33]. The results of this study that total WBC count was associated with postprandial glucose levels may reflect such postprandial inflammatory changes.…”

Section: Discussionsupporting

confidence: 49%

The association of total and differential white blood cell count with metabolic syndrome in type 2 diabetic patients

Shim¹,

Kim²,

Kang³

et al. 2006

Diabetes Research and Clinical Practice

102

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“…Both excessive eating and consuming a poor quality diet are associated with more systemic inflammatory stress. In the nonpregnant state, meals and especially fat rich meals cause transient inflammatory stress 63,64 which may be sustained if the intervals between meals are short.…”

Section: Oxidative and Inflammatory Stress And The Onset Of Preeclampsiamentioning

confidence: 99%

Micronutrients in pregnancy: Current knowledge and unresolved questions

et al. 2011

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One-carbon metabolism Pregnancy Liposoluble vitamins Minerals s u m m a r yMicronutrient status is increasingly recognized to play an important role in the health and well-being of pregnant women and in the development and long-term health of the offspring. On 26th e 28th February 2009, The Child Health Foundation invited leading experts in this area to a scientific workshop at Obergurgl, Austria to review and critically discuss current knowledge, to identify issues that may need to be addressed in future recommendations, and to highlight priorities and opportunities for future research. This report summarizes updated key conclusions of the workshop with regards to micronutrients' intake and physiological role related to mother, placenta and fetus, as well as relevance for adverse pregnancy and long-term outcomes.

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Postprandial recruitment of neutrophils may contribute to endothelial dysfunction

Cited by 226 publications

References 53 publications

Modulation of gene expression in endothelial cells by hyperlipaemic postprandial serum from healthy volunteers

Modulation of gene expression in endothelial cells by hyperlipaemic postprandial serum from healthy volunteers

The association of total and differential white blood cell count with metabolic syndrome in type 2 diabetic patients

Micronutrients in pregnancy: Current knowledge and unresolved questions

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