Postnatal Development of the Mouse Volatile Papilla Taste Bud Cells

Amasaki, Hajime; Arai, Rie; Ogawa, Miyuki; Takemura, Naoyuki; Yamagami, Takeshi; Nagasao, Jun; Mutoh, Ken-ichiro; Ichihara, Nobutsune; Asari, Masao

doi:10.1292/jvms.65.541

Cited by 7 publications

(6 citation statements)

References 11 publications

(12 reference statements)

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“…It is secreted into saliva by serous acinar cells of the parotid, submandibular (Parkkila et al 1990), and lingual von Ebner's glands (Amasaki et al 2003;Leinonen et al 2001) and into milk by the mammary glands (Karhumaa et al 2001). Recently this enzyme has been found in the lacrimal glands (Ogawa et al 2002), nasal, septal, and lateral glands (Kimoto et al 2004), esophagus (Kasuya et al 2007;Murakami et al 2003), tracheobronchial glands, epithelial serous cells (Leinonen et al 2004), and alimentary canal (Kaseda et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

et al. 2010

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Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and CAVI may have additional functions that have yet to be discovered. To explore the functions of CAVI more fully, we generated Car6 (-/-) mice and analyzed Car6 (-/-) mutant phenotypes. We also examined transcriptomic responses to CAVI deficiency in the submandibular gland, stomach, and duodenum of Car6 (-/-) mice. Car6 (-/-) mice were viable and fertile and had a normal life span. Histological analyses indicated a greater number of lymphoid follicles in the small intestinal Peyer's patches. A total of 94, 56, and 127 genes were up- or down-regulated in the submandibular gland, stomach, and duodenum of Car6 (-/-) mice, respectively. The functional clustering of differentially expressed genes revealed a number of altered biological processes. In the duodenum, the significantly affected biological pathways included the immune system process and retinol metabolic processes. The response to oxidative stress and brown fat cell differentiation changed remarkably in the submandibular gland. Notably, the submandibular gland, stomach, and duodenum shared one important transcriptional susceptibility pathway: catabolic process. Real-time PCR confirmed an altered expression in 14 of the 16 selected genes. The generation and of Car6 (-/-) mice and examination of the effects of CAVI deficiency on gene transcription have revealed several affected clusters of biological processes, which implicate CAVI in catabolic processes and the immune system response.

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Section: Introductionmentioning

confidence: 99%

Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

et al. 2010

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“…It was firstly purified by Feldstein and Silverman [6] from rat saliva, and later by Murakami and Sly [20], Kadoya et al [13], and Parkkila et al [25] from human, and Asari et al [5] from bovine saliva. Previous immunohistochemical analysis has demonstrated the cellular and intracellular origins of CA-VI in the parotid and mandibular glands of humans, mice, rats, and sheep [8,24,25,27], in developing parotid glands of cattle [5], in the lacrimal gland of rat [23], and in the lingual serous von Ebner's glands of humans, rats [18], and mice [2]. CA-VI has been detected in bovine mammary gland [11,16] and in rat lower airways and lung [18,19].…”

Section: Resultsmentioning

confidence: 99%

Immunohistochemistry of the Bovine Secretory Carbonic Anhydrase Isozyme (CA-VI) in Bovine Alimentary Canal and Major Salivary Glands

Kaseda

Ichihara

Nishita

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. In the present study, we firstly demonstrated immunohistochemical expressions of secretory carbonic anhydrase (CA-VI) isozyme in bovine forestomach, large intestine and major salivary glands. CA-VI was detected in basal layer epithelial cells of esophageal and forestomach stratified epithelium, in mucous cells of upper glandular region of large intestine, in serous acinar cells of the parotid gland, in serous demilune cells and some ductal liner cells of mandibular, monostomatic sublingual and esophageal glands. These immunohistolocalizations suggested that bovine CA-VI plays various roles in pH regulation, maintenance of ion and fluid balance, and cell proliferation. KEY WORDS: bovine digestive organs, CA-VI, immunohistochemical localization.J. Vet. Med. Sci. 68(2): 131-135, 2006 Carbonic anhydrase isozyme-VI (CA-VI) is a unique secretory type CA isozyme. It was initially discovered and purified from ovine saliva [7], and then rat saliva [6,21], human saliva [13,20,22,25] and bovine saliva [5]. Fernley et al. [8] examined sheep CA-VI in 18 different tissues from sheep using biochemical analysis. They observed an immunoreactive signal for CA-VI only in parotid and mandibular glands, which suggested that CA-VI increased buffering capacity in the oral cavity. Some studies have identified the acinar cells of salivary glands as the origin of CA-VI in several species [5,8,21,22,25]. These studies have shown that CA-VI was present in acinar cells of the mammalian parotid and mandibular glands, from where CA-VI is secreted into the saliva. However, CA-VI expression in other segments of the alimentary canal has received little information and the precise physiological role of bovine CA-VI has never been revealed. The present study examines the immunohistochemical expression of CA-VI in bovine alimentary canal and in major salivary glands, and conjectures the function of CA-VI in the bovine digestive organs. MATERIALS AND METHODSTissue: Three adult Holstein cows were used in the present study. Small tissue blocks were prepared for microscopic observation. Samples were cut from the parotid, mandibular, and monostomatic sublingual glands, from cervical to thoracic segments of the esophagus, rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon and rectum. The samples were immediately fixed in Bouin's solution, and then sliced into 3 µm-thick sections. CA-VI antibody:We purified CA-VI from bovine saliva as described previously [5]. A rabbit polyclonal antibody has been raised against purified bovine CA-VI and specific immunity to bovine CA-VI has been confirmed [5]. Briefly, the antibody against purified bovine CA-VI was produced in the rabbit. Rabbits were injected initially with 1 mg of purified CA-VI emulsified with an equal volume of Freund's complete adjuvant, followed by a weekly booster injection of a fixed amount of the enzyme for 5 weeks. The rabbits were bled via the auricular vein 10 days following the last injection. The specificity of the antiserum was confirmed by the doubl...

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“…3M∼3O). At NE21, the mandibular molar tooth erupted into the Completion of taste bud in fungiform papillae 19,27) Functional stage Weaning phase 19,20) oral cavity and was at a functional stage (Fig. 3P).…”

Section: ) Neonatal Day 21 (Ne21)mentioning

confidence: 99%

Comparative Morphological Study on the Embryonic and Neonatal Development of the Filiform Papillae and Teeth in Mice

Jeong

2020

J Dent Hyg Sci

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Background: In the early stages of development, teeth and lingual papillae are induced and developed through special and complex epithelial-mesenchymal interactions. Tooth completion indicates the beginning of the weaning phase, and accordingly, many oral tissues and organs are completed, and it is thought that their developmental completion times are related to each other. The purpose of this study was to clarify the embryonic and neonatal development of the filiform papillae and mandibular molar tooth, and discuss the developmental relationship between these organs by comparing the developmental completion times. Methods: Embryos at embryonic day 15 (EM15), 17 (EM17), and 21 (EM21) and mice at neonatal day 1 (NE1), 5 (NE5), 10 (NE10), and 21 (NE21) were used for experimentation. Tissues dissected from embryos and mice were fixed, and processed for histological analysis. Sections from the tissues were stained with hematoxylin and eosin for observation under a light microscope. Results: Based on the histological analysis results, the developmental process of the lingual epithelium covering the dorsal surface of the tongue was classified into three stages: initiation, morphogenesis, and functional. The development of the filiform papillae begins at EM17; undergoes rapid morphological changes in epithelial cells at EM21, PN1 and PN5, and reaches the functional stage at PN10, which is the sucking phase. Tooth development begins at EM13 or 15 and is completed at NE21 through prenatal and postnatal development. Conclusion: The development of the filiform papillae was initiated late and completed quickly through embryonic and neonatal development in comparison with the mandibular molar tooth. The filiform papillae are considered to play an important role in sucking rather than mastication as it is completed in the sucking phase.

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Postnatal Development of the Mouse Volatile Papilla Taste Bud Cells

Cited by 7 publications

References 11 publications

Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

Immunohistochemistry of the Bovine Secretory Carbonic Anhydrase Isozyme (CA-VI) in Bovine Alimentary Canal and Major Salivary Glands

Comparative Morphological Study on the Embryonic and Neonatal Development of the Filiform Papillae and Teeth in Mice

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