Postnatal changes in expression of vesicular glutamate transporters in the main olfactory bulb of the rat

Ohmomo, Hideki; Ina, Ayuka; Yoshida, Shigeo; Shutoh, Fumihiro; Ueda, Seinosuke; Hisano, Setsuji

doi:10.1016/j.neuroscience.2009.02.048

Cited by 11 publications

(14 citation statements)

References 45 publications

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“…Given the ubiquitous nature of the amino acid glutamate, in situ hybridization for VGluTs has come to be recognized as the most reliable method to label the cell bodies of glutamatergic neurons [25]. In agreement with recent findings in the rat OB [26], we found that, in the mouse OB, VGluT1 and 2 showed marked differences in their distributions through the OB layers. A larger proportion of VGluT1-positive cells was observed in the mitral cell layer, as well as strong expression at the boundary of the GL and EPL.…”

Section: Resultssupporting

confidence: 89%

Sequential generation of olfactory bulb glutamatergic neurons by Neurog2-expressing precursor cells

Winpenny¹,

Lebel-Potter

Fernández

et al. 2011

Neural Dev

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BackgroundWhile the diversity and spatio-temporal origin of olfactory bulb (OB) GABAergic interneurons has been studied in detail, much less is known about the subtypes of glutamatergic OB interneurons.ResultsWe studied the temporal generation and diversity of Neurog2-positive precursor progeny using an inducible genetic fate mapping approach. We show that all subtypes of glutamatergic neurons derive from Neurog2 positive progenitors during development of the OB. Projection neurons, that is, mitral and tufted cells, are produced at early embryonic stages, while a heterogeneous population of glutamatergic juxtaglomerular neurons are generated at later embryonic as well as at perinatal stages. While most juxtaglomerular neurons express the T-Box protein Tbr2, those generated later also express Tbr1. Based on morphological features, these juxtaglomerular cells can be identified as tufted interneurons and short axon cells, respectively. Finally, targeted electroporation experiments provide evidence that while the majority of OB glutamatergic neurons are generated from intrabulbar progenitors, a small portion of them originate from extrabulbar regions at perinatal ages.ConclusionsWe provide the first comprehensive analysis of the temporal and spatial generation of OB glutamatergic neurons and identify distinct populations of juxtaglomerular interneurons that differ in their antigenic properties and time of origin.

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Section: Resultssupporting

confidence: 89%

Sequential generation of olfactory bulb glutamatergic neurons by Neurog2-expressing precursor cells

Winpenny¹,

Lebel-Potter

Fernández

et al. 2011

Neural Dev

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“…Intense vGluT1 and vGluT2 mRNA signals were observed in numerous cells located in the mitral cell layer, the external plexiform layer and the glomerular layer, but not in the GCL32, 34 (Fig. 6a-e; Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Adult generation of glutamatergic olfactory bulb interneurons

Brill

Ninkovic

Winpenny³

et al. 2009

Nat Neurosci

319

346

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The adult mouse subependymal zone (SEZ) harbours neural stem cells that are thought to generate exclusively GABAergic interneurons of the olfactory bulb. Here we describe the adult generation of glutamatergic juxtaglomerular neurons, with dendritic arborizations that project into adjacent glomeruli identifying them as short-axon cells. Fate mapping revealed that these originate from Neurogenin2- and Tbr2-expressing progenitors located in the dorsal region of the SEZ. Progenitors of these glutamatergic interneurons recapitulate the sequential expression of transcription factors that hallmark glutamatergic neurogenesis in the developing cerebral cortex and adult hippocampus. Indeed, the molecular specification of these SEZ progenitors allows for their recruitment into the cerebral cortex upon lesion. Taken together, our data show that SEZ progenitors not only produce a novel population of adult-born glutamatergic juxtaglomerular neurons, but may also provide a new source of progenitors for endogenous repair.

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“…As reported in several brains regions 36-38 , the expression of VGLUTs in the GL of the OB is developmentally regulated [Fig. 2; 18 ]. From our study it emerges that VGLUT3 neurons represent the smallest population of glutamatergic cells in the GL, but its density increases with age.…”

Section: Discussionmentioning

confidence: 61%

“…VGLUT1 neurons had larger somata (Fig. 3a-d) and may represent tufted cells (TCs) 18, 19 . In contrast, TOM + and VGLUT2 + cells could not be easily distinguished from each other but had larger soma than GABAergic neurons identified by the expression of the vesicular GABA transporter (VGAT; Fig.…”

Section: Resultsmentioning

confidence: 99%

A population of glomerular glutamatergic neurons controls sensory information transfer in the mouse olfactory bulb

et al. 2014

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In sensory systems, peripheral organs convey sensory inputs to relay networks where information is shaped by local microcircuits before being transmitted to cortical areas. In the olfactory system, odorants evoke specific patterns of sensory neuron activity which are transmitted to output neurons in olfactory bulb glomeruli. How sensory information is transferred and shaped at this level remains still unclear. Here we employ mouse genetics, 2-photon microscopy, electrophysiology and optogenetics, to identify a novel population of glutamatergic neurons (VGLUT3+) in the glomerular layer of the adult mouse olfactory bulb as well as several of their synaptic targets. Both peripheral and serotoninergic inputs control VGLUT3+ neurons firing. Furthermore, we show that VGLUT3+ neurons photostimulation in vivo strongly suppresses both spontaneous and odor-evoked firing of bulbar output neurons. In conclusion, we identify and characterize here a microcircuit controlling the transfer of sensory information at an early stage of the olfactory pathway.

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Postnatal changes in expression of vesicular glutamate transporters in the main olfactory bulb of the rat

Cited by 11 publications

References 45 publications

Sequential generation of olfactory bulb glutamatergic neurons by Neurog2-expressing precursor cells

Sequential generation of olfactory bulb glutamatergic neurons by Neurog2-expressing precursor cells

Adult generation of glutamatergic olfactory bulb interneurons

A population of glomerular glutamatergic neurons controls sensory information transfer in the mouse olfactory bulb

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