Postischemic treatment bytrans-resveratrol in rat liver ischemia-reperfusion: A possible strategy in liver surgery

Abstract

Liver ischemia-reperfusion (I/R) injury occurs in many clinical conditions, including liver surgery and transplantation. Oxygen free radicals generated during I/R reduce endogenous antioxidant systems and contribute to hepatic injury. trans-Resveratrol (trans-3,5,4'-trihydroxystilbene) is reported to have antioxidant properties. We investigated the effect of trans-resveratrol on liver injury induced by I/R. After 1 hour of ischemia, administered 5 minutes before 3 hours of reperfusion, trans-resveratrol was hepatoprotective at a low dose (0.02 mg/kg). It significantly decreased aminotransferase levels by about 40% and improved sinusoidal dilatation. trans-Resveratrol preserved antioxidant defense by preventing total and reduced glutathione depletion caused by I/R. At 0.2 mg/kg, trans-resveratrol significantly increased glutathione reductase, Cu/Zn-superoxide dismutase, and catalase activities. However, at a high dose (20 mg/kg), trans-resveratrol became prooxidant with an aggravation of liver injury evaluated by aminotransferase release and histological analysis and associated with a depletion of total and reduced glutathione levels and a decrease of antioxidant enzyme activities. In conclusion, a prereperfusion treatment by trans-resveratrol only at low doses decreases liver injury induced by I/R by protecting against antioxidant defense failure. This administration protocol could reduce liver damage during surgery or transplantation.

Role of food-derived antioxidant agents against acetaminophen-induced hepatotoxicity

“…It has been shown to protect against liver damage caused by hepatic ischemia-reperfusion (Plin et al 2005;Hassan-Khabbar et al 2008), ethanol toxicity (Ajmo et al 2008Min et al 2008), highfat diet (Ahn et al 2008) and hepatotoxic agents such as APAP (Sener et al 2006;Masubuchi et al 2009) and carbon tetrachloride (Rivera et al 2008). The mechanisms that may explain this protection include antioxidant effects, up-regulation of phase II enzymes, modulation of inflammatory responses and induction of mitochondrial biogenesis (Baur et al 2006;Sener et al 2006;Lagouge et al 2006;Masubuchi et al 2009;Bishayee et al 2010).…”

Resveratrol attenuates oxidative stress and histological alterations induced by liver ischemia/reperfusion in rats

“…Histopathologically, the resveratrol-treated group showed well preserved liver parenchyma with hepatocytes arranged radially around the central vein. In a study by Hassan-Khabbar et al [50] , the effect of trans-resveratrol on liver injury induced by I/R was investigated. While Hassan-Khabbar performed measurements after 3 h of reperfusion, in the present study, measurements were performed after less than 1 h. Thus, the present study suggests that the protective effect of resveratrol may occur more rapidly than previously thought.…”

Nigella sativa relieves the deleterious effects of ischemia reperfusion injury on liver

“…This remains a significant problem for surgical procedures, and also remains limitation of liver transplantation [3] . Oxygen free radicals, produced on reperfusion, play a critical role in the injury caused by ischemia-reperfusion [4] . Reactive oxygen radicals lead to an inflammatory response and tissue damage by activating some mediators.…”