Postharvest rot and mummification of strawberry fruits caused by Neofusicoccum parvum and N. kwambonambiense in Brazil

Lopes, Ueder Pedro; Zambolim, Laércio; Pinho, D. B.; Barros, Alexandre Guimarães de Almeida; Costa, Hélcio; Pereira, Olinto Liparini

doi:10.1590/s1982-56762014000200009

Cited by 13 publications

(6 citation statements)

References 19 publications

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“…N. brasiliense has been described in Northeast Brazil associated with fruit rot in mango (Marques et al 2013); thus, this is the second report of this species in Brazil and the first in guava branches. N. kwambonambiense was described for the first time in South Africa in asymptomatic stems, leaves and fruits of dead plants of Syzygium cordatum (Pavilic et al 2009, Phillips et al 2013 and was later reported in grapevines in Uruguay (Abreo et al 2013), and to cause fruit rot in post-harvested strawberry in Brazil (Lopes et al 2014). The occurrence of N. kwambonambiense associated with symptoms of dieback and canker in stems of cashew plants is unprecedented in Brazilian territory.…”

Section: Discussionmentioning

confidence: 99%

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An emended description of Neofusicoccum brasiliense and characterization of Neoscytalidium and Pseudofusicoccum species associated with tropical fruit plants in northeastern Brazil

et al. 2018

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The Botryosphaeriaceae family is one of the most important groups of plant pathogenic fungi associated with tropical woody species such as Anacardium occidentale (cashew) and Mangifera indica (mango), which are fruiting species with broad distributions and high economic importance in north-eastern Brazil. This study was designed to characterize species of this family associated with cashew, mango and Psidium guajava (guava) that cause dieback and stem and branch cankers. Characterization comprised phylogenetic, morphological, physiological and pathogenic features. The phylogenetic study combined sequences of the internal transcribed spacer (ITS) of the rDNA and partial elongation factor 1-α (EF1-α) regions of DNA. Measurements of fungal conidia and colony growth on different culture media and at different temperatures were conducted. Pathogenicity tests were also performed through inoculation of different host species. By combining the sequences of the referenced genes, it was possible to identify the following Botryosphaeriaceae species: Neofusicoccum kwambonambiense, Neoscytalidium hyalinum and Pseudofusicoccum stromaticum as the first report in cashew, Neoscytalidium hyalinum in mango and Neofusicoccum brasiliense as the first report in guava. Examination of N. brasiliense revealed conidia, providing the first morphological description of the species. Neofusicoccum kwambonambiense did not sporulate, but the other isolates produced conidia with dimensions comparable to those described in the literature. All isolates were virulent to mango fruits and young cashew and “caja-umbu” plants, causing necrotic lesions and gum exudation by the hosts. The isolates of Neofusicoccum were the most aggressive in the inoculated hosts.

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Section: Discussionmentioning

confidence: 99%

“…The association of these genera with pathosystems has recently increased (Lopes et al 2014, Gonçalves et al 2016. Twenty-two species of Neofusicoccum (Berraf-Tebbal et al 2014), two of Neoscytalidium and six of Pseudofusicoccum have been reported in Brazil (Phillips et al 2013).…”

Section: Introductionmentioning

confidence: 99%

An emended description of Neofusicoccum brasiliense and characterization of Neoscytalidium and Pseudofusicoccum species associated with tropical fruit plants in northeastern Brazil

et al. 2018

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“…One of the limiting factors that influence its economic value is the relatively short shelf life caused by postharvest spoilage (Al-Najada and Gherbawy 2015 ). Fungi were considered the main reason for strawberry postharvest spoilage (Lopes et al 2014 ). The fungal species causing postharvest spoilage in strawberries vary according to the region, weather and crop management (Maas 1998 ).…”

Section: Introductionmentioning

confidence: 99%

Mycobiota associated with strawberry fruits, their mycotoxin potential and pectinase activity

2020

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Forty-three species and variety belonging to 15 genera were collected from 30 strawberry fruit samples on Glucose-Czapek's agar medium. Among them, Aspergillus flavus, Aspergillus niger and Penicillium citrinum were the most frequent species recovered from 53.3%, 70.0% and 50.0% of the samples, respectively. According to the ITS rDNA sequence, we confirmed the morphological identification result. Moreover, aflatoxin biosynthesis gene omt-A was detected in A. flavus, while Aopks gene was found in A. niger. Interestingly, we could not detect any aflatoxin or ochratoxin biosynthesis genes in the P. citrinum strain. The concentration of detected aflatoxin was 3.5 ppb produced by A. flavus, while A. niger gave 4.1 ppb as ochratoxin. A. flavus was the most pectinase producer among the selected strains, and the highest amount was obtained at 30°C after 6 days of incubation with initial medium pH 8.

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“…During our study of forest pathology, we observed a serious disease occurring in the leaves of Machilus thunbergii plants. The pathogen was identified through morphological and molecular approaches by following methods applied earlier [27][28][29][30][31]. Leaf spot disease caused by the fungal pathogen Neofusicoccum parvum has been found in other forest plants, including eucalyptus in Spain, blueberry in Korea, cannabis in Italy, and pine in Australia [13,[27][28][29].…”

Section: Discussionmentioning

confidence: 99%

Morphology, Molecular Phylogeny, and Pathogenicity of Neofusicoccum parvum, Associated with Leaf Spot Disease of a New Host, the Japanese Bay Tree (Machilus thunbergii)

Choi

Paul

Lee

et al. 2021

Forests

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During a survey of diseased plants on Wando Island, Korea from May to June 2020, a severe leaf spot disease was observed in the upper leaves of Japanese bay tree (Machilus thunbergii). Early symptoms were light blackish spots on the leaf surface and enlargement of older spots. Dry leaf spots surrounded with deep black margins were common throughout the plants. Symptomatic leaf samples were collected, and the causal pathogen was isolated on potato dextrose agar (PDA). Three fungal isolates (CMML20-1, CMML20-3, and CMML20-4) were cultured on PDA for morphological characterization at 25 °C in the darkness. Fungal colonies were circular, fast-growing, olivaceous to dark grey, and with abundant aerial mycelium. Sporulation was induced in 14 h-10 h light-dark conditions, and the conidia were single-celled, thin-walled with a smooth surface, ellipsoid with round apices, and measuring 17.5–20.5 (avg. 17.5) μm × 7.5–10.0 (7.9) μm. The morphological characteristics resembled those typical for Neofusicoccum parvum. Molecular identification was confirmed by partially sequencing the internal transcribed spacer (ITS) region and the translation elongation factor 1-α (EF1-α) genes. Pathogenicity tests were conducted on detached leaves and whole plants of M. thunbergii. High disease prevalence was observed, and Koch postulates were fulfilled. This is the first worldwide report of N. parvum causing leaf spots on Machilus thunbergii.

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Postharvest rot and mummification of strawberry fruits caused by Neofusicoccum parvum and N. kwambonambiense in Brazil

Cited by 13 publications

References 19 publications

An emended description of Neofusicoccum brasiliense and characterization of Neoscytalidium and Pseudofusicoccum species associated with tropical fruit plants in northeastern Brazil

An emended description of Neofusicoccum brasiliense and characterization of Neoscytalidium and Pseudofusicoccum species associated with tropical fruit plants in northeastern Brazil

Mycobiota associated with strawberry fruits, their mycotoxin potential and pectinase activity

Morphology, Molecular Phylogeny, and Pathogenicity of Neofusicoccum parvum, Associated with Leaf Spot Disease of a New Host, the Japanese Bay Tree (Machilus thunbergii)

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