Posterior rat eye during acute intraocular pressure elevation studied using polarization sensitive optical coherence tomography

Fialová, Stanislava; Augustin, Marco; Fischak, Corinna; Schmetterer, Leopold; Handschuh, Stephan; Glösmann, Martin; Pircher, Michael; Hitzenberger, Christoph K.; Baumann, Bernhard

doi:10.1364/boe.8.000298

Cited by 14 publications

(15 citation statements)

References 50 publications

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“…Furthermore, the effects may differ with age. 47,48 Different metrics, such as retinal layer thickness, 49 retinal capillary density, 14 birefringence, 50 or gene expression, 51 can be affected to different degrees by IOP elevation. We believe that vis-OCT, which has the ability to provide structural information, as well as data on perfusion of major retinal vessels and capillary beds and total retinal blood flow, oximetry, oxygen extraction, and oxygen metabolism, offers several advantages that may help in understanding these phenomena.…”

Section: Discussionmentioning

confidence: 99%

Monitoring retinal responses to acute intraocular pressure elevation in rats with visible light optical coherence tomography

Hormel

Wei

et al. 2019

Neurophoton.

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Elevated intraocular pressure (IOP) is an important risk factor for glaucoma. However, the role of IOP in glaucoma progression, as well as retinal physiology in general, remains incompletely understood. We demonstrate the use of visible light optical coherence tomography to measure retinal responses to acute IOP elevation in Brown Norway rats. We monitored retinal responses in reflectivity, angiography, blood flow, oxygen saturation (sO 2), and oxygen metabolism over a range of IOP from 10 to 100 mmHg. As IOP was elevated, nerve fiber layer reflectivity was found to decrease. Vascular perfusion in the three retinal capillary plexuses remained steady until IOP exceeded 70 mmHg and arterial flow was noted to reverse periodically at high IOPs. However, a significant drop in total retinal blood flow was observed first at 40 mmHg. As IOP increased, the venous sO 2 demonstrated a gradual decrease despite steady arterial sO 2 , which is consistent with increased arterial-venous oxygen extraction across the retinal capillary beds. Calculated total retinal oxygen metabolism was steady, reflecting balanced responses of blood flow and oxygen extraction, until IOP exceeded 40 mmHg, and fell to 0 at 70 and 80 mmHg. Above this, measurements were unattainable. All measurements reverted to baseline when the IOP was returned to 10 mmHg, indicating good recovery following acute pressure challenge. These results demonstrate the ability of this system to monitor retinal oxygen metabolism noninvasively and how it can help us understand retinal responses to elevated IOP.

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Section: Discussionmentioning

confidence: 99%

Monitoring retinal responses to acute intraocular pressure elevation in rats with visible light optical coherence tomography

Hormel

Wei

et al. 2019

Neurophoton.

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“…In this work we utilized a multifunctional OCT imaging system that was described in detail by Fialová et al 43 and has been recently used to image mouse 41 and rat models 42,44 of ophthalmic diseases. In short, the system is based on a SD, PS-OCT unit enabling high-speed retinal imaging at 83-kHz Ascan rate with an axial resolution of 3.8 lm in retinal tissue.…”

Section: Multifunctional Optical Coherence Tomographymentioning

confidence: 99%

In Vivo Characterization of Spontaneous Retinal Neovascularization in the Mouse Eye by Multifunctional Optical Coherence Tomography

Augustin

Wechdorn

Pfeiffenberger

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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“…ONH and lamina cribrosa biomechanics are also often studied using optical coherence tomography, which has the important advantage that it can be used in vivo. 1,41,42 However, despite exciting advances, such as the integration of polarized light information, 43,44 the light-penetration, resolution, and contrast of these systems are not yet sufficient to quantitatively analyze lamina cribrosa beams with the level of detail demonstrated with SPLM in this work. A study using serial sectioning of eyes fixed at various intraocular pressures followed by PLM imaging has addressed some of these issues, and indeed provided valuable findings.…”

Section: Discussionmentioning

confidence: 99%

Structured polarized light microscopy for collagen fiber structure and orientation quantification in thick ocular tissues

et al. 2018

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Collagen is a major constituent of the eye and understanding its architecture and biomechanics is critical to preserve and restore vision. We, recently, demonstrated polarized light microscopy (PLM) as a powerful technique for measuring properties of the collagen fibers of the eye, such as spatial distribution and orientation. Our implementation of PLM, however, required sectioning the tissues for imaging using transmitted light. This is problematic because it limits analysis to thin sections. This is not only slow, but precludes study of dynamic events such as pressure-induced deformations, which are central to the role of collagen. We introduce structured polarized light microscopy (SPLM), an imaging technique that combines structured light illumination with PLM to allow imaging and measurement of collagen fiber properties in thick ocular tissues. Using pig and sheep eyes, we show that SPLM rejects diffuse background light effectively in thick tissues, significantly enhancing visualization of optic nerve head (ONH) structures, such as the lamina cribrosa, and improving the accuracy of the collagen fiber orientation measurements. Further, we demonstrate the integration of SPLM with an inflation device to enable direct visualization, deformation tracking, and quantification of collagen fibers in ONHs while under controlled pressure.

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Posterior rat eye during acute intraocular pressure elevation studied using polarization sensitive optical coherence tomography

Cited by 14 publications

References 50 publications

Monitoring retinal responses to acute intraocular pressure elevation in rats with visible light optical coherence tomography

Monitoring retinal responses to acute intraocular pressure elevation in rats with visible light optical coherence tomography

In Vivo Characterization of Spontaneous Retinal Neovascularization in the Mouse Eye by Multifunctional Optical Coherence Tomography

Structured polarized light microscopy for collagen fiber structure and orientation quantification in thick ocular tissues

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