In Vivo Characterization of Spontaneous Retinal Neovascularization in the Mouse Eye by Multifunctional Optical Coherence Tomography

Augustin, Marco; Wechdorn, Matthias; Pfeiffenberger, Ulrike; Himmel, Tanja; Fialová, Stanislava; Werkmeister, René M.; Hitzenberger, Christoph K.; Glösmann, Martin; Baumann, Bernhard

doi:10.1167/iovs.17-23208

Cited by 15 publications

(9 citation statements)

References 60 publications

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“…3 In disease models of human retinal pathology, PS-OCT has also proven a valuable contrast enhancement and an additional route toward the quantitative assessment of tissue parameters and lesion properties. 39,40,57 Although PS-OCT has been successfully demonstrated for imaging the human anterior eye in health and disease by multiple experiments and studies, this is-to the best of our knowledge-the first presentation of PS-OCT in the anterior segment of murine eyes.…”

Section: Discussionmentioning

confidence: 96%

Polarization-sensitive optical coherence tomography imaging of the anterior mouse eye

et al. 2018

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Polarization-sensitive optical coherence tomography (PS-OCT) enables noninvasive, high-resolution imaging of tissue polarization properties. In the anterior segments of human eyes, PS-OCT allows the visualization of birefringent and depolarizing structures. We present the use of PS-OCT for imaging the murine anterior eye. Using a spectral domain PS-OCT setup operating in the 840-nm regime, we performed in vivo volumetric imaging in anesthetized C57BL/6 mice. The polarization properties of murine anterior eye structures largely replicated those known from human PS-OCT imagery, suggesting that the mouse eye may also serve as a model system under polarization contrast. However, dissimilarities were found in the depolarizing structure of the iris which, as we confirmed in postmortem histological sections, were caused by anatomical differences between both species. In addition to the imaging of tissues in the anterior chamber and the iridocorneal angle, we demonstrate longitudinal PS-OCT imaging of the murine anterior segment during mydriasis as well as birefringence imaging of corneal pathology in an aged mouse.

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Section: Discussionmentioning

confidence: 96%

Polarization-sensitive optical coherence tomography imaging of the anterior mouse eye

et al. 2018

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“…Although the tissue preparation process can cause folding or shrinkage in histology, OCT is known to suffer from artefacts caused by, for example, sample motion or poor signal quality (e.g., in case of cataract). 26,33 In 5 out of 18 eyes from the CTRL, we found hyperreflective lesions. Although spontaneous age-related changes might be one explanation for these findings, another reason could be inherited defects owing to the genetic background of the wildtype CTRL.…”

Section: Discussionmentioning

confidence: 78%

“…This observation is further substantiated by the regular appearance of the RPE in the DOPU images. Although PS-OCT has been proven capable of detecting pigmentation changes such as the migration of melanin [25][26][27] or the elevation of the RPE as in the case of soft drusen, 28,29 the drusenlike lesions observed in the SOD1 KO mice exhibited polarization characteristics similar to those found in patients with pseudodrusen: a polarization-preserving, hyper-reflective focal deposit overlying the depolarizing RPE layer (Fig. 5).…”

Section: Discussionmentioning

confidence: 98%

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Optical Coherence Tomography Findings in the Retinas of SOD1 Knockout Mice

Augustin

Harper

Merkle

et al. 2020

Trans. Vis. Sci. Tech.

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The retinal phenotype of popular mouse models mimicking ophthalmic diseases, such as the superoxide dismutase 1 (SOD1) knockout (KO) mouse model, has mainly been assessed by ex vivo histology and in vivo fundus photography. We used multifunctional optical coherence tomography (OCT) to characterize the retinas of SOD1 KO mice in vivo. Methods: The custom-made ophthalmoscope featured a combination of conventional OCT, polarization-sensitive OCT, and OCT angiography. Seven SOD1 KO mice and nine age-matched controls were imaged between 6 and 17 months of age. A postprocessing framework was used to analyze total and outer retinal thickness changes. Drusenlike lesions were segmented, and their sizes and the number of lesions were assessed quantitatively. Their appearance in the conventional reflectivity images, as well as in the corresponding polarization-sensitive images, was characterized qualitatively. Results: Drusenlike lesions increased in size and number with age for SOD1 KO mice. Exploiting the multiple contrast channels, the appearance of the lesions was found to resemble pseudodrusen observed in eyes of patients suffering from dry age-related macular degeneration. The total and outer retinal thicknesses were lower on average after 11 months and 7 months in SOD1 KO mice compared with age-matched controls. Neovascularizations were found in one out of seven KO animals. Conclusions: OCT imaging proved beneficial for a detailed in vivo characterization of the pathological changes in SOD1 KO mice. Translational Relevance: Phenotyping of animal models using modern imaging concepts can be conducted with more precision and might also ease the translation of conclusions between clinical and preclinical research.

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“…The examination of the visual pathway, as a part of the central nervous system, has been proven to be an effective starting point in addressing not only ophthalmologic 1,2,3,4,5 , but also neurologic 6,7,8,9,10,11,12,13,14,15,16 questions. In recent years, OCT and OKR have been identified as useful analytic, non-invasive tools to evaluate a large variety of retinopathies and retinal manifestations in various rodent models 17,18,19,20,21,22,23,24,25 . OCT allows for fast and high resolution in vivo visualization of the retinal morphology and structure in mice and rats, with results in good accordance with histological sections of the animals retinae 26 .…”

Section: Introductionmentioning

confidence: 99%

Using Optical Coherence Tomography and Optokinetic Response As Structural and Functional Visual System Readouts in Mice and Rats

Dietrich

Hecker

Hilla

et al. 2019

JoVE

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Optical coherence tomography (OCT) is a fast, non-invasive, interferometric technique allowing high-resolution retinal imaging. It is an ideal tool for the investigation of processes of neurodegeneration, neuroprotection and neuro-repair involving the visual system, as these often correlate well with retinal changes. As a functional readout, visually evoked compensatory eye and head movements are commonly used in experimental models involving the visual function. Combining both techniques allows a quantitative in vivo investigation of structure and function, which can be used to investigate the pathological conditions or to evaluate the potential of novel therapeutics. A great benefit of the presented techniques is the possibility to perform longitudinal analyses allowing the investigation of dynamic processes, reducing variability and cuts down the number of animals needed for the experiments. The protocol described aims to provide a manual for acquisition and analysis of high quality retinal scans of mice and rats using a low cost customized holder with an option to deliver inhalational anesthesia. Additionally, the proposed guide is intended as an instructional manual for researchers using optokinetic response (OKR) analysis in rodents, which can be adapted to their specific needs and interests.

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In Vivo Characterization of Spontaneous Retinal Neovascularization in the Mouse Eye by Multifunctional Optical Coherence Tomography

Cited by 15 publications

References 60 publications

Polarization-sensitive optical coherence tomography imaging of the anterior mouse eye

Polarization-sensitive optical coherence tomography imaging of the anterior mouse eye

Optical Coherence Tomography Findings in the Retinas of SOD1 Knockout Mice

Using Optical Coherence Tomography and Optokinetic Response As Structural and Functional Visual System Readouts in Mice and Rats

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