Posterior Capsule Opacification in Pseudophakic Eyes

McDonnell, Peter J.; Zarbin, Marco A.; Green, W. Richard

doi:10.1016/s0161-6420(83)34350-5

Cited by 279 publications

(100 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Of note, EMT and stress fiber formation of LECs were observed in anterior subcapsular fibrosis of human cataracts and posterior capsule opacity after cataract surgery (16,65,72). Our findings regarding ROS-induced TGF-␤-mediated induction of Tm may thus be associated with cataractogenesis and posterior-capsular opacification after cataract surgery.…”

Section: Prdx6mentioning

confidence: 56%

Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure

Kubo¹,

Hasanova²,

Tanaka

et al. 2010

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Oxidative stress is one of the causative factors in progression and etiology of agerelated cataract. Peroxiredoxin 6 (Prdx6), a savior for cells from internal or external environmental stresses, plays a role in cellular signaling by detoxifying reactive oxygen species (ROS) and thereby controlling gene regulation. Using targeted inactivation of the Prdx6 gene, we show that Prdx6-deficient lens epithelial cells (LECs) are more vulnerable to UV-triggered cell death, a major cause of skin disorders including cataractogenesis, and these cells display abnormal protein profiles. PRDX6-depleted LECs showed phenotypic changes and formed lentoid body, a characteristic of terminal cell differentiation and epithelial-mesenchymal transition. Prdx6 Ϫ/Ϫ LECs exposed to UV-B showed higher ROS expression and were prone to apoptosis compared with wild-type LECs, underscoring a protective role for Prdx6. Comparative proteomic analysis using fluorescence-based difference gel electrophoresis along with mass spectrometry and database searching revealed a total of 13 proteins that were differentially expressed in Prdx6 Ϫ/Ϫ cells. Six proteins were upregulated, whereas expression of seven proteins was decreased compared with Prdx6LECs. Among the cytoskeleton-associated proteins that were highly expressed in Prdx6-deficient LECs was tropomyosin (Tm)2␤. Protein blot and real-time PCR validated dramatic increase of Tm2␤ and Tm1␣ expression in these cells. Importantly, Prdx6 ϩ/ϩ LECs showed a similar pattern of Tm2␤ protein expression after transforming growth factor (TGF)-␤ or H2O2 treatment. An extrinsic supply of PRDX6 could restore Tm2␤ expression, demonstrating that PRDX6 may attenuate adverse signaling in cells and thereby maintain cellular homeostasis. Exploring redox-proteomics (Prdx6 Ϫ/Ϫ ) and characterization and identification of abnormally expressed proteins and their attenuation by PRDX6 delivery should provide a basis for development of novel therapeutic interventions to postpone ROS-mediated abnormal signaling deleterious to cells or tissues.peroxiredoxin; oxidative stress; proteomics; tropomyosin OXIDATIVE STRESS has been identified as one of the major causes of age-related diseases, including cataracts (1, 45, 62, 74 -76, 82). Oxidative stress-induced etiology and progression of diseases may result either from diminished natural antioxidants such as catalase, glutathione peroxidase, and peroxiredoxins (PRDXs) due to aging or from increased generation of reactive oxygen species (ROS) by exposure to environmental factors, X-rays, chemicals, toxins, and ultraviolet (UV) radiation. Environmental stressors such as these have been heavily implicated in the etiology and progression of several diseases by inducing ROS-mediated oxidative stress.UV radiation exposure induces several complex sets of acute or chronic responses that can lead to initiation of diseases through overproduction of ROS. ROS modify cellular signaling. Modulation of signal transduction pathways includes changes in gene expression (18). However, investigation...

show abstract

Section: Prdx6mentioning

confidence: 56%

Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure

Kubo¹,

Hasanova²,

Tanaka

et al. 2010

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

show abstract

“…Each patient was advised during this visit to attend for an eye examination with their favoured optometrist for a change in spectacle lenses as required. Although the interval between cataract surgery and the appearance of PCO varies considerably, McDonnell et al 21 have reported that PCO usually does not occur until at least 3 months post-operatively, with about 50% of 10L implant patients developing PCO within 5 years. Since we were interested in the visual outcomes of the surgery unhindered by PCO, the chosen post-operative period should have been a relatively stable period in which eyes had recovered sufficiently from the effects of the surgery and were unlikely to have developed PCO.…”

Section: Methodsmentioning

confidence: 99%

A comparison of visual performance between a rigid PMMA and a foldable acrylic intraocular lens

Afsar

Patel

Woods

et al. 1999

Eye

View full text Add to dashboard Cite

show abstract

“…It is formed due to the migration of equatorial capsular epithelial cells towards the posterior capsule. PCO occurs as result of formation of opaque secondary membrane by lens epithelial cell proliferation, transformation of lens epithelial cells into fibroblasts with contractile element and collagen deposition [1]. PCO is the most common delayed complication of cataract surgery that causes decreased vision, glare and other symptoms similar to that of original cataract; PCO causes reduction in visual acuity and contrast sensitivity by obstructing the view or by scattering the light that is perceived as glare by the patient.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Different Techniques and Energy Used in Nd: Yag Laser Posterior Capsulotomy on Intraocular Pressure: A Prospective Study

Fahim¹

2017

Austin J Clin Ophthalmol

View full text Add to dashboard Cite

Purpose: To study the relationship between energy used and its effect on IOP in different capsulotomy techniques i.e. cruciate, circular and inverted 'u'. Subjects and Methods:The study was conducted in 154 patients from the out patients department who fulfilled the inclusion criteria and developed posterior capsular opacification following successful cataract surgery. And the patients were randomly divided into three groups. Group I undergoing cruciate technique, group II undergoing circular technique and group III undergoing inverted U technique. Preprocedure intraocular pressure was recorded by using applanation tonometry. Patients undergoing Nd: Yag laser capsulotomy were seen after 2 hours, 1 day, 1week, 1 month and 3 months and IOP was recorded. The outcome was measured in terms of number of shots given in the technique, total energy used and intra ocular pressure changes after the laser. Results:In the light of our present study, we have found that group II which is circular technique group consumes higher no. of laser shots and higher amount of total laser energy which leads to transient rise in intraocular pressure. Correlation was found between the IOP readings obtained and amount of total laser energy used. Conclusions:The techniques cruciate and inverted U appears to be better and safer than the circular technique in terms of amount of energy used and the complications associated with it.

show abstract

Posterior Capsule Opacification in Pseudophakic Eyes

Cited by 279 publications

References 10 publications

Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure

Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure

A comparison of visual performance between a rigid PMMA and a foldable acrylic intraocular lens

The Effects of Different Techniques and Energy Used in Nd: Yag Laser Posterior Capsulotomy on Intraocular Pressure: A Prospective Study

Contact Info

Product

Resources

About