Postcolumn chemiluminescence, ultraviolet and evaporative light-scattering detectors in high-performance liquid chromatographic determination of triacylglycerol oxidation products

Mäkinen, Marjukka; Piironen, Vieno; Hopia, Anu

doi:10.1016/0021-9673(95)01298-2

Cited by 35 publications

(18 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Firstly, a wide range of hydroperoxides can be analysed: molecules of different volatility, molecular size and polarity. The technique has been applied to fatty acids [62,63] [63][64][65][66][67][68], fatty acid methyl esters [69][70][71], phospholipids [63,[72][73][74][75][76], cholesterol and cholesterol esters [63,77] using either normal or reverse phase columns.…”

Section: Liquid Chromatographymentioning

confidence: 99%

“…Also, different detection systems have been used for the analysis of hydroperoxides in model systems and biological samples. Thus UV detection at 230-235 nm [66,69,70], evaporative light-scattering detector [66] and an electrochemical detector [65,72] have been employed. However, the most interesting approach to increase the sensitivity and specificity of hydroperoxide analysis has been the use of post-column reactions [6].…”

Section: Liquid Chromatographymentioning

confidence: 99%

“…The decomposition of hydroperoxides to the corresponding alkoxy radicals is catal-ysed by heme compounds (i.e. cytochrome c or microperoxidase), and subsequent oxidation of luminol or isoluminol is accompanied by the emission of light [62,63,66,71,[74][75][76][77].…”

Section: Liquid Chromatographymentioning

confidence: 99%

See 2 more Smart Citations

Analysis of lipid hydroperoxides

Dobarganes

Velasco

2002

Eur. J. Lipid Sci. Technol.

122

View full text Add to dashboard Cite

Section: Liquid Chromatographymentioning

confidence: 99%

Section: Liquid Chromatographymentioning

confidence: 99%

See 1 more Smart Citation

Analysis of lipid hydroperoxides

Dobarganes

Velasco

2002

Eur. J. Lipid Sci. Technol.

122

View full text Add to dashboard Cite

“…These products play an important role in many pathological processes such as atherosclerosis, cancer, Alzheimer's and Parkinson disease [1][2][3][4]. Hydroperoxides further react forming heterogeneous mixtures of volatile, monomeric and polymeric products [5,6]. Among many analytical methods there is no standard method to detect all oxidative changes in foods.…”

Section: Introductionmentioning

confidence: 99%

Influence of native antioxidants on the formation of fatty acid hydroperoxides in model systems

Nogala‐Kałucka

Kupczyk

Polewski³

et al. 2007

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

The effect of alpha-tocopherol (alpha-T) and quercetin on the formation of hydroperoxides of linoleic and linolenic acids during autoxidation at 60 6 1 7C was investigated. Three isomers of hydroperoxides were detected using HPLC. Of isomers of linoleic acid hydroperoxides, 13-hydroperoxy-octadecadienoic acid trans-trans (13-HPODE tt), 9-HPODE cis-trans (9-HPODE c-t) and 9-HPODE trans-trans (9-HPODE t-t) were identified, constituting 64, 19 and 17% of the total amount, respectively. For linolenic acid, the components 13-hydroperoxy-octadecatrienoic acid trans-trans (13-HPO-TE t-t), 9-HPOTE c-t and 9-HPOTE t-t contributed 7, 33 and 60% to the total, respectively. The different dominant hydroperoxide isomers detected in linoleic and linolenic acids during oxidation are related to their chemical structure and the microenvironment of emulsion droplets. The ratios between specific isomers for both fatty acid hydroperoxides did not change during oxidation with or without antioxidants. Alpha-T effectively inhibited the oxidation of fatty acids and reduced the formation of hydroperoxides. The total amount of the hydroperoxides decreased along with the increase in the concentration of alpha-T, 1-40 mM. Quercetin inhibited the oxidation of both fatty acids at similar efficiency only at 40 mM concentration. A synergistic antioxidant effect of quercetin with alpha-T in a binary system on both fatty acids was observed.

show abstract

“…For HPLC coupled with some newly developed detection technologies, such as chemiluminescence (CL) and evaporative light scattering (ELC) detectors [14,15], the detection sensitivity was obviously enhanced. In addition, efforts focusing on the improvement of separation efficiency for chromatographic columns have also emerged [16,17].…”

Section: Introductionmentioning

confidence: 99%

Determining the purity of samples from natural products by coupling HPLC and CCD spectrometry

Chen

Shen

Kong³

2005

J. Sep. Science

View full text Add to dashboard Cite

Isolation and identification of natural products is a very important and active research field. However, establishing the purity of the samples during the isolation process is quite difficult, especially when the retention times are similar for two desired components in HPLC. Although some technologies, e.g. MS and NMR, offer effective ways of obtaining purity information about the samples, the expensive instrumentation required or the off-line nature of coupling (generally speaking) make purity analysis somewhat inconvenient. In this paper, an on-line analytical system coupling HPLC and a CCD spectrometer for determination of purity for each eluate was developed in a thin layer spectrometric cell. The effectiveness of the system was demonstrated by differentiating Tanshinone I, Tanshinone IIA, and their mixture. The time-resolved UV-Vis spectra promptly revealed significant differences between the three samples while conventional single wavelength detection (CSWLD) could not. The system was then used to distinguish two steroid compounds which behaved as a single component in CSWLD. The compounds were isolated from a Chinese marine invertebrate animal, a marine annelid, Arenicola cristata, referred to here as Stimpson. The method reported here provided an efficient, convenient, fast, and inexpensive approach holding promise for on-line determination of the purity of samples isolated from natural products.

show abstract

Postcolumn chemiluminescence, ultraviolet and evaporative light-scattering detectors in high-performance liquid chromatographic determination of triacylglycerol oxidation products

Cited by 35 publications

References 23 publications

Analysis of lipid hydroperoxides

Analysis of lipid hydroperoxides

Influence of native antioxidants on the formation of fatty acid hydroperoxides in model systems

Determining the purity of samples from natural products by coupling HPLC and CCD spectrometry

Contact Info

Product

Resources

About