Post-translational modifications of Abutilon mosaic virus movement protein (BC1) in fission yeast

Kleinow, Tatjana; Holeiter, Gerlinde; Nischang, Marc; Stein, Monika; Karayavuz, Miriam; Wege, Christina; Jeske, Holger

doi:10.1016/j.virusres.2007.08.011

Cited by 12 publications

(12 citation statements)

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“…PCP has been reported previously to perform some MP functions, such as intracellular movement and cell periphery localization [9], [25], [26]. Additionally, in-vitro phosphorylation activity was reported in MP of Abutilon mosaic virus [33]. Our thorough bioinformatic comparison of geographically separated begomovirus species has produced a candidate region for detailed wet lab analysis.…”

Section: Discussionmentioning

confidence: 84%

Bioinformatic Analysis Reveals Genome Size Reduction and the Emergence of Tyrosine Phosphorylation Site in the Movement Protein of New World Bipartite Begomoviruses

Kuchie

Duffy

2014

PLoS ONE

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Begomovirus (genus Begomovirus, family Geminiviridae) infection is devastating to a wide variety of agricultural crops including tomato, squash, and cassava. Thus, understanding the replication and adaptation of begomoviruses has important translational value in alleviating substantial economic loss, particularly in developing countries. The bipartite genome of begomoviruses prevalent in the New World and their counterparts in the Old World share a high degree of genome homology except for a partially overlapping reading frame encoding the pre-coat protein (PCP, or AV2). PCP contributes to the essential functions of intercellular movement and suppression of host RNA silencing, but it is only present in the Old World viruses. In this study, we analyzed a set of non-redundant bipartite begomovirus genomes originating from the Old World (N = 28) and the New World (N = 65). Our bioinformatic analysis suggests ∼120 nucleotides were deleted from PCP’s proximal promoter region that may have contributed to its loss in the New World viruses. Consequently, genomes of the New World viruses are smaller than the Old World counterparts, possibly compensating for the loss of the intercellular movement functions of PCP. Additionally, we detected substantial purifying selection on a portion of the New World DNA-B movement protein (MP, or BC1). Further analysis of the New World MP gene revealed the emergence of a putative tyrosine phosphorylation site, which likely explains the increased purifying selection in that region. These findings provide important information about the strategies adopted by bipartite begomoviruses in adapting to new environment and suggest future in planta experiments.

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Section: Discussionmentioning

confidence: 84%

Bioinformatic Analysis Reveals Genome Size Reduction and the Emergence of Tyrosine Phosphorylation Site in the Movement Protein of New World Bipartite Begomoviruses

Kuchie

Duffy

2014

PLoS ONE

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“…The successful expression of ACMV CP in fission yeast extends the list of several geminiviral proteins that were produced in this organism [ 35 , 36 , 46 , 47 , 48 , 49 ]. S. pombe is a more suitable model organism than Saccharomyces cerevisiae and bacteria because its molecular mechanisms are more closely related to those of plants concerning post-translational modifications, chromatin structure, and RNA processing [ 50 , 51 , 52 , 53 , 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast

Hipp

Schafer

Kepp

et al. 2016

Viruses

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The capsid proteins (CPs) of geminiviruses combine multiple functions for packaging the single-stranded viral genome, insect transmission and shuttling between the nucleus and the cytoplasm. African cassava mosaic virus (ACMV) CP was expressed in fission yeast, and purified by SDS gel electrophoresis. After tryptic digestion of this protein, mass spectrometry covered 85% of the amino acid sequence and detected three N-terminal phosphorylation sites (threonine 12, serines 25 and 62). Differential centrifugation of cell extracts separated the CP into two fractions, the supernatant and pellet. Upon isopycnic centrifugation of the supernatant, most of the CP accumulated at densities typical for free proteins, whereas the CP in the pellet fraction showed a partial binding to nucleic acids. Size-exclusion chromatography of the supernatant CP indicated high order complexes. In DNA binding assays, supernatant CP accelerated the migration of ssDNA in agarose gels, which is a first hint for particle formation. Correspondingly, CP shifted ssDNA to the expected densities of virus particles upon isopycnic centrifugation. Nevertheless, electron microscopy did not reveal any twin particles, which are characteristic for geminiviruses.

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“…It moves across the cell wall to adjacent uninfected cells through the plasmodesmata (Rojas et al ., 1998). MP of BDMV (Lee, 2008), like those of Tobacco mosaic virus (TMV) (Trutnyeva et al ., 2005; Waigmann et al ., 2000), AbMV (Kleinow et al ., 2008) and others, can be phosphorylated at its C terminus by cell wall‐associated kinases. This may be necessary for increasing the plasmodesmata size exclusion limit, allowing viral cell‐to‐cell movement.…”

Section: Cell‐to‐cell Movementmentioning

confidence: 99%

“…Lee (2008) identified a host protein kinase, termed plasmodesmal‐associated protein kinase (PAPK), which can phosphorylate both TMV and BDMV MPs. It has been proposed (Kleinow et al ., 2008) that there are multiple phosphorylation sites on AbMV MP and that the virus exploits the cell signal transduction and signalling pathways via protein phosphorylation during plant infection. Furthermore, the phosphorylation status may vary during infection according to different kinase activities in different plant tissues and developmental stages of the plant.…”

Section: Cell‐to‐cell Movementmentioning

confidence: 99%

“…Furthermore, the phosphorylation status may vary during infection according to different kinase activities in different plant tissues and developmental stages of the plant. Kleinow et al . (2008) have suggested a model in which the phosphorylation process switches off MP following successful transfer, leading to the inhibition of its transport capability.…”

Section: Cell‐to‐cell Movementmentioning

confidence: 99%

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Bean dwarf mosaic virus: a model system for the study of viral movement

Levy¹,

Tzfira

2010

Molecular Plant Pathology

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The symptom intensity in common bean plants depends on the stage of infection. Early infection of susceptible bean seedlings will result in severe stunting and dwarfing, leaf distortion and mottling or mosaic, as well as chlorotic or yellow spots or blotches. BDMV-infected plants usually abort their flowers or produce severely distorted pods. Late infection of susceptible plants or early infection of moderately resistant genotypes may show a mild mosaic, mottle and crumpling or an irregular distribution of variegated patches. BIOLOGICAL PROPERTIES: As a member of the Begomovirus group, BDMV is transmitted from plant to plant by the whitefly Bemisia tabaci. BDMV is a nonphloem-limited virus and can replicate and move in the epidermal, cortical and phloem cells. As a nonphloem-limited virus, it is sap-transmissible.

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Post-translational modifications of Abutilon mosaic virus movement protein (BC1) in fission yeast

Cited by 12 publications

References 68 publications

Bioinformatic Analysis Reveals Genome Size Reduction and the Emergence of Tyrosine Phosphorylation Site in the Movement Protein of New World Bipartite Begomoviruses

Bioinformatic Analysis Reveals Genome Size Reduction and the Emergence of Tyrosine Phosphorylation Site in the Movement Protein of New World Bipartite Begomoviruses

Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast

Bean dwarf mosaic virus: a model system for the study of viral movement

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