Post‐mortem recovery, in vitro maturation and fertilization of fallow deer (<i>Dama dama</i>, Linnaeus 1758) oocytes collected during reproductive and no reproductive season

Uccheddu, Stefania; Pintus, Eliana; Garde, José Julián; Fleba, Lidia; Muzzeddu, Marco; Pudda, Flavia; Bogliolo, Luisa; Strina, Alessandro; Nieddu, Stefano; Ledda, Sergio

doi:10.1111/rda.13773

Cited by 3 publications

(2 citation statements)

References 57 publications

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“…Apoptotic sperm can be also responsible for the loss of human embryos in the early stages of development [ 33 ]. Therefore, the highest number of cleaved embryos and blastocysts we measured was in the group fertilized with sperm stored for one day, and the results were lower than those reported by other authors who conducted IVF with cryopreserved sperm [ 34 , 35 ]. Thus, storage-induced changes in sperm, embryonic development affected by oocyte quality, in vitro culture conditions such as temperature, and the applied culture media [ 36 , 37 ] are all aspects demanding further study.…”

Section: Discussioncontrasting

confidence: 70%

The Quality and Fertilizing Potential of Red Deer (Cervus elaphus L.) Epididymal Spermatozoa Stored in a Liquid State

Dziekońska

Koziorowska-Gilun

Kordan

et al. 2022

IJMS

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The aim of this study was to assess the quality and fertilizing potential of red deer epididymal spermatozoa stored in a liquid state for up to 11 days (D11). In Experiment 1, sperm quality was determined. In Experiment 2, the efficiency of in vitro fertilization (IVF) and artificial insemination (AI) of stored sperm were evaluated. An analysis of sperm quality on D5 of storage revealed a decrease (p < 0.05) in motility and morphology, and a higher proportion of apoptotic spermatozoa. On D1, D7 and D10, the total motility of sperm for IVF and AI was determined to be 82.6%, 71.0% and 64.8%, respectively. The results of IVF and AI demonstrated that the fertilizing potential of spermatozoa differs between days of storage. The percentage of blastocysts was higher when oocytes were fertilized on D1 (17.4 %) compared to D7 (8.5%) and D10 sperm (10.5%). Differences were noted in the pregnancy rates of inseminated hinds. The insemination with D1, D7 and D10 sperm led to live births (33% from D7 and D10). The results indicate that the quality of red deer epididymal spermatozoa remains satisfactory during ten days of storage in a liquid state, and that these spermatozoa maintain their fertility potential.

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Section: Discussioncontrasting

confidence: 70%

The Quality and Fertilizing Potential of Red Deer (Cervus elaphus L.) Epididymal Spermatozoa Stored in a Liquid State

Dziekońska

Koziorowska-Gilun

Kordan

et al. 2022

IJMS

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“…Moreover, the fertilization rate depends on the reproductive status when oocytes are collected; results of our study [25] and the Berg and Asher [41] study showed low fertilization rates during the no-breeding season. The content of the medium during IVF also plays an important role because even if successful fertilization occurs, further embryo development may be inhibited or finished before the blastocyst stage when the culture conditions are not proper, as in a study on fallow deer [42]. In the case of cervids, of interest is research connected with the differences in zona pellucida (ZP) content in selected Cervidae species that may cross naturally, e.g., Cervus elaphus and Cervus nippon [43].…”

Section: In Vitro Maturation (Ivm)mentioning

confidence: 99%

Artificial Reproductive Technology (ART) Applied to Female Cervids Adapted from Domestic Ruminants

Korzekwa

Kotlarczyk

2021

Animals

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There are about 150 Cervidae species on the IUCN Red List of Threatened Species. Only a small part is counted among farm animals, and most of them are free roaming. The universality and large numbers of representatives of cervids such as red deer (Cervus elaphus) and roe deer (Capreolus capreolus) may predispose these species to be used as models for research on reintroduction or assisted reproduction of deer at risk of extinction. We outlined the historical fluctuation of cervids in Europe and the process of domestication, which led to breeding management. Consequently, the reproductive techniques used in domestic ruminants were adapted for use in female deer which we reviewed based on our results and other available results. We focused on stress susceptibility in cervids depending on habitat and antropopression and proposed copeptin as a novel diagnostic parameter suitable for stress determination. Some reproductive biotechniques have been adopted for female cervids with satisfactory results, e.g., in vitro fertilization, while others still require methodological refinement, e.g., cryopreservation of oocytes and embryos.

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Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats

Souza-Fabjan

Correia

Batista

et al. 2021

Animals

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Reproductive seasonality may have a considerable influence on the efficiency of assisted reproductive technologies in seasonal species. This study evaluated the effect of season on cleavage, blastocyst rates and quality of in vitro produced (IVP) goat embryos. In total, 2348 cumulus–oocyte complexes (COCs) were recovered from slaughterhouse ovaries and subjected to the same IVP system throughout 1.5 years (49 replicates). The odds ratio (OR) among seasons was calculated from values of cleavage and blastocyst rates in each season. Cleavage rate was lower (p < 0.05) in spring (anestrus), in comparison with either autumn (peak of breeding season) or summer, while the winter had intermediate values. Furthermore, lower OR of cleavage was observed in spring. Blastocyst formation rate (from initial number of COCs) was higher (p < 0.05) in autumn (52 ± 2.5%) when compared with the other seasons (combined rates: 40 ± 1.9%). Moreover, its OR was higher (p < 0.05) in autumn compared to all other seasons and impaired in the spring compared to winter (OR: 0.54) and summer (OR: 0.48). Embryo hatchability and blastocyst cell number were similar (p > 0.05) among seasons. In conclusion, the breeding season leads to improved oocyte developmental competence, resulting in higher cleavage and blastocyst yield, whereas embryo quality remained similar throughout the years.

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Post‐mortem recovery, in vitro maturation and fertilization of fallow deer (Dama dama, Linnaeus 1758) oocytes collected during reproductive and no reproductive season

Cited by 3 publications

References 57 publications

The Quality and Fertilizing Potential of Red Deer (Cervus elaphus L.) Epididymal Spermatozoa Stored in a Liquid State

The Quality and Fertilizing Potential of Red Deer (Cervus elaphus L.) Epididymal Spermatozoa Stored in a Liquid State

Artificial Reproductive Technology (ART) Applied to Female Cervids Adapted from Domestic Ruminants

Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats

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