Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats

Souza-Fabjan, J. M. G.; Correia, L.; Batista, Ríbrio Ivan Tavares Pereira; Locatelli, Yann; Freitas, Vicente José de Figueirêdo; Mermillod, Pascal

doi:10.3390/ani11030873

Cited by 4 publications

(14 citation statements)

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“…From 25-36 months to the age of >36 months, muscle tissue plays a role in growth. The bone grows continuously with a relatively slow rate of growth and relatively faster muscle growth [23], so the muscle-to-bone ratio increases during growth [24].…”

Section: A Body Weight Of Kacang Goats and Jawarandu Goatsmentioning

confidence: 99%

Characteristics of Phenotypes of Local Goat Quantitative Properties in East Kolaka Regency, Indonesia

Pagala¹,

Rahim²,

Aku³

et al. 2023

Int. J. Adv. Sci. Eng. Inf. Technol.

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Goats are a type of livestock that most people mostly keep to produce meat and milk to meet the protein needs of animal origin. Even local goats, such as kacang and jawarandu goats in East Kolaka Regency are used as a source of community income, especially education costs. This study aims to determine the quantitative characteristics of local goats in the East Kolaka Regency, carried out in three sub-districts: Ladongi District, Lambandia District, and Uluiwoi District, from January to April 2021. The research sample was 496 male and female local goats grouped by age < 1 year to > 3 years to determine quantitative characteristics in the form of bodyweight, chest circumference, shoulder height, and body length. Data and information were tabulated and analyzed descriptively, and a T-test was performed. The results of T-test analysis for body weights of kacang and jawarandu goats in the age group showed significant differences (P<0.05) in both aged 6-12 months, 13-24 months, 25-36 months and >36 months, including chest circumference, shoulder height, and body length, while the diversity of head sizes of kacang and jawarandu goats including head length, head width, ear length, and ear width there was no significant difference (P>0, 05) between the head length of male and female goats aged 6-12 months, 13-24 months, 25-36 months and >36 months. So, it can be concluded that between kacang and jawarandu goats, there are differences in quantitative characteristics.

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Section: A Body Weight Of Kacang Goats and Jawarandu Goatsmentioning

confidence: 99%

Characteristics of Phenotypes of Local Goat Quantitative Properties in East Kolaka Regency, Indonesia

Pagala¹,

Rahim²,

Aku³

et al. 2023

Int. J. Adv. Sci. Eng. Inf. Technol.

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“…Although MOET is still more predominant (64% vs 36%), the number of IVEP-derived embryos has increased by ~300% from 2019 to 2021 (Figure 1), and the efficiency of producing embryos per doe was quite similar in both techniques [7.7 (MOET) vs 6.4 (IVEP)]. The reasons why MOET remains the main technique for small ruminant embryo production are probably related to species particularities compared to cattle, costs involved, and outcomes (Souza-Fabjan et al, 2021). In general, the IVEP comprises four main steps: (1) the collection of cumulus-oocyte complexes (COCs); (2) COCs in vitro maturation (IVM); (3) in vitro fertilization (IVF), that is the co-cultivation of COCs and sperm; and (4) in vitro culture (IVC) or in vitro development (IVD) of the embryos until the blastocyst stage when they can be transferred to the uterus of synchronized female recipients (with pregnancy rates around 50%) or cryopreserved for future use, according to each production system (Cognié et al, 2004;Souza-Fabjan et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…For anatomical reasons, in small ruminants, the ovum pick-up is performed laparoscopically (LOPU). Despite it is considered a minimally invasive technique (Baldassarre, 2012;Souza-Fabjan et al, 2014b), performing LOPU is more expansive and complex than the bovine ovum pick-up (OPU; Souza-Fabjan et al, 2021), which certainly contributes to the lower use of IVEP in small ruminants.…”

Section: Introductionmentioning

confidence: 99%

“…It is well known that oocyte quality is essential for IVEP success (Kątska-Książkiewicz et al, 2007), being influenced by several factors, such as age, nutritional and health status, the hormonal protocol used for superstimulation, among others (Paramio and Izquierdo, 2016;Bragança et al, 2018). In small ruminants, however, the breeding season has a substantial impact on oocyte developmental competence, reflecting positively on IVEP results (Souza-Fabjan et al, 2021). Furthermore, despite advances in IVF, most protocols are currently based on concepts established many years ago, reinforcing the demand for optimizing the semen capacitation process to improve pregnancy rates (Cognié et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Although there are obviously some peculiarities and adaptations in each step, the results regarding IVEP rates in small ruminants are similar to those reported in cattle. It means that there is no reason for MOET to be still considered virtually the unique technique to produce embryos (Souza-Fabjan et al, 2021), and changes in the global scenario are expected, as experienced in goats recently. This review aims to describe the main factors affecting the outcomes in each IVEP step, in order to identify what is still missing for its wider dissemination in goats and sheep.…”

Section: Introductionmentioning

confidence: 99%

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In vitro embryo production in small ruminants: what is still missing?

Souza-Fabjan,

Leal,

Monteiro

et al. 2023

Anim. Reprod.

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In vitro embryo production (IVEP) is an extremely important tool for genetic improvement in livestock and it is the biotechnology that has grown the most recently. However, multiple ovulation followed by embryo transfer is still considered the leading biotechnology for embryo production in small ruminants. This review aimed to identify what is still missing for more efficient diffusion of IVEP in small ruminants, going through the IVEP steps and highlighting the main factors affecting the outcomes. Oocyte quality is essential for the success of IVEP and an aspect to be considered in small ruminants is their reproductive seasonality and strategies to mitigate the effect of season. The logistics for oocyte collection from live females is more complex than in cattle, and tools to simplify this collection system and/or to promote an alternative way of recovering oocytes may be an important point in this scenario. The heterogeneity of oocytes collected from growing follicles in live females or from ovaries collected from abattoirs remains a challenge, and there is a demand to standardize/homogenize the hormonal stimulatory protocols and IVM protocols for each source of oocytes. The use of sexed semen is technically possible, however the low market demand associated with the high costs of the sexing process prevents the routine use of this technique, but its higher availability is an important aspect aiming for greater dissemination of IVEP. New noninvasive approaches for embryo selection are key factors since the selection for transfer or cryopreservation is another difficulty faced among laboratories. Embryo selection is based on morphological traits, although these are not necessarily reliable in predicting pregnancy. Several issues described in this review must be considered by researchers in other to promote the diffusion of IVEP in small ruminants.

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