Post-maturation treatment improves and synchronizes somatic embryo germination of three species of Japanese pines

Maruyama, Tsuyoshi; Hosoi, Yoshihisa

doi:10.1007/s11240-012-0128-7

Cited by 35 publications

(18 citation statements)

References 31 publications

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“…Many studies have shown a positive effect of drying/ desiccation of mature somatic embryos of conifers on germination and conversion Hogberg et al 2001;Pond et al 2002;Szczygieł et al 2007;Maruyama and Hosoi 2012). Roberts with co-workers (1990) were able to increase the germination efficiency of somatic embryos of interior spruce (a mixture of Picea glauca and P. engelmannii from the interior of British Columbia) to 90 % following treatment at humidities greater than 95 %.…”

Section: Discussionmentioning

confidence: 99%

“…7 Typical somatic seedling of Norway spruce (Picea abies), with first needles visible around the apical bud, obtained after partial drying of embryos at 97 % relative humidity. Bar 10 mm Maruyama and Hosoi (2012) improved germination and conversion rate of somatic embryos of the three Japanese pine species (Pinus thunberghii, P. densiflora, and P. armandii var. amamiana) using the slow drying of embryos at high relative humidity (98 %).…”

Section: Discussionmentioning

confidence: 99%

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Effect of partial drying and desiccation on somatic seedling quality in Norway and Serbian spruce

et al. 2014

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Somatic embryo quality is still a problem for many researchers. To improve the efficiency of germination, special procedures are used, such as partial drying of somatic embryos at high relative humidity or desiccation in the presence of supersaturated solutions of salt. In this work, cotyledonary somatic embryos of Norway spruce (Picea abies) and Serbian spruce (P. omorika) were placed on culture media (ME or BM-5) to germinate. We found that after 4 weeks of incubation on these media, hypocotyl and radicle growth of control (non-dried) somatic embryos of both species was not adequate to yield seedlings able to acclimatize to greenhouse conditions. Therefore, somatic embryos were partly dried at relative humidity of 97 % or desiccated at relative humidity of 79 %, for 2 or 3 weeks, and then placed on the Margara (ME) medium. Partial drying of somatic embryos at the higher relative humidity (97 %) enabled an improvement of radicle growth of germinating somatic embryos in both species. The highest conversion rate (45 %) was obtained for embryos of Norway spruce maintained for 2 weeks at relative humidity of 97 %. This treatment contributed to the improvement of germination and conversion efficiency of somatic embryos of Norway spruce, regardless of the drying period. Improved radicle growth facilitated development of better quality seedlings of this spruce species. In Serbian spruce, we did not obtain seedlings of sufficient quality, due to poor hypocotyl growth. Desiccation at humidity of 79 % for 3 weeks proved to be lethal to somatic embryos of both species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of partial drying and desiccation on somatic seedling quality in Norway and Serbian spruce

et al. 2014

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“…This treatment has been used effectively to improve the germination/conversion of somatic embryos in Picea abies (Bozhkov and Von Arnold, ; Find, ; Högberg et al ., ), P. rubens (Harry and Thorpe, ), P. glauca (Attree et al ., ; Kong and Yeung, ), P. mariana (Beardmore and Charest, ) and the Pinus species, P. patula (Jones and van Staden, ), P. thunbergii , P. densiflora and P. armandii var. amamiana (Maruyama and Hosoi, ), as well as Abies nordmanniana (Nørgaard, ; Salajova and Salaj, ; Vooková and Kormuťák, ).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of stress‐related proteins and metabolic pathways in Picea asperata somatic embryos during partial desiccation

Jing

Zhang

Xia

et al. 2016

Plant Biotechnology Journal

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SummaryPartial desiccation treatment (PDT) stimulates germination and enhances the conversion of conifer somatic embryos. To better understand the mechanisms underlying the responses of somatic embryos to PDT, we used proteomic and physiological analyses to investigate these responses during PDT in Picea asperata. Comparative proteomic analysis revealed that, during PDT, stress‐related proteins were mainly involved in osmosis, endogenous hormones, antioxidative proteins, molecular chaperones and defence‐related proteins. Compared with those in cotyledonary embryos before PDT, these stress‐related proteins remained at high levels on days 7 (D7) and 14 (D14) of PDT. The proteins that differentially accumulated in the somatic embryos on D7 were mapped to stress and/or stimuli. They may also be involved in the glyoxylate cycle and the chitin metabolic process. The most significant difference in the differentially accumulated proteins occurred in the metabolic pathways of photosynthesis on D14. Furthermore, in accordance with the changes in stress‐related proteins, analyses of changes in water content, abscisic acid, indoleacetic acid and H2O2 levels in the embryos indicated that PDT is involved in water‐deficit tolerance and affects endogenous hormones. Our results provide insight into the mechanisms responsible for the transition from morphologically mature to physiologically mature somatic embryos during the PDT process in P. asperata.

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“…This is in contrast to studies of somatic embryo maturation of Picea abies , in which PEG is reported to stimulate embryogenesis but inhibit the subsequent germination process [ 72 ]. Partial desiccation and/or cold treatments after maturation on medium containing PEG have been reported necessary to improve somatic embryo germination and conversion in a number of conifer species, including some pines [ 73 , 74 , 75 , 76 , 77 , 78 , 79 ], spruces [ 80 , 81 , 82 , 83 ], hybrid larch [ 84 ], Fraser fir [ 66 ], and Chinese fir [ 85 ].…”

Section: Resultsmentioning

confidence: 99%

Somatic Embryogenesis and Plant Regeneration from Sugi (Japanese Cedar, Cryptomeria japonica D. Don, Cupressaceae) Seed Families by Marker Assisted Selection for the Male Sterility Allele ms1

Maruyama

Ueno

Hirayama

et al. 2020

Plants

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One of the possible countermeasures for pollinosis caused by sugi (Cryptomeria japonica), a serious public health problem in Japan, is the use of male sterile plants (MSPs; pollen-free plants). However, the production efficiencies of MSPs raised by conventional methods are extremely poor, time consuming, and resulting in a high seedling cost. Here, we report the development of a novel technique for efficient production of MSPs, which combines marker-assisted selection (MAS) and somatic embryogenesis (SE). SE from four full sib seed families of sugi, carrying the male sterility gene MS1, was initiated using megagametophyte explants that originated from four seed collections taken at one-week intervals during the month of July 2017. Embryogenic cell lines (ECLs) were achieved in all families, with initiation rates varying from 0.6% to 59%. Somatic embryos were produced from genetic marker-selected male sterile ECLs on medium containing maltose, abscisic acid (ABA), polyethylene glycol (PEG), and activated charcoal (AC). Subsequently, high frequencies of germination and plant conversion (≥76%) were obtained on plant growth regulator-free medium. Regenerated plantlets were acclimatized successfully, and the initial growth of male sterile somatic plants was monitored in the field.

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Post-maturation treatment improves and synchronizes somatic embryo germination of three species of Japanese pines

Cited by 35 publications

References 31 publications

Effect of partial drying and desiccation on somatic seedling quality in Norway and Serbian spruce

Effect of partial drying and desiccation on somatic seedling quality in Norway and Serbian spruce

Proteomic analysis of stress‐related proteins and metabolic pathways in Picea asperata somatic embryos during partial desiccation

Somatic Embryogenesis and Plant Regeneration from Sugi (Japanese Cedar, Cryptomeria japonica D. Don, Cupressaceae) Seed Families by Marker Assisted Selection for the Male Sterility Allele ms1

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