Proteomic analysis of stress‐related proteins and metabolic pathways in <i>Picea asperata</i> somatic embryos during partial desiccation

Jing, Danlong; Zhang, Jianwei; Xia, Yan; Kong, Lisheng; Ouyang, Fangqun; Zhang, Shougong; Zhang, Hanguo; Wang, Junhui

doi:10.1111/pbi.12588

Cited by 38 publications

(40 citation statements)

References 56 publications

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“…Consistent with our findings, genes encoding auxin-responsive protein exhibited significantly increased abundance under partial desiccation treatment [94]. It is well recorded that ABA is a critical messenger that mediates the adaptive response of plants to abiotic stresses [95]. When plants are subjected to drought stress, a large number of ABA-regulated genes are accumulated.…”

Section: Genes Related To Hormone Signaling Are Critical For Drought supporting

confidence: 90%

Global transcriptome and weighted gene co-expression network analyses of growth-stage-specific and hybrid-cultivar-specific drought stress responses in maize

Liu¹,

Zenda²,

Jin³

et al. 2020

Preprint

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Background Drought is the major abiotic stress threatening maize ( Zea mays L.) production globally. Therefore, deciphering the molecular basis of maize drought tolerance remains pertinent. Results Here, through a comprehensive comparative leaf transcriptome analysis of drought-tolerant hybrid ND476 plants subjected to water-sufficient (control) and water-deficit (drought) treatment conditions at four (V12, VT, R1, and R4) crop growth stages, we report key cultivar-specific and growth-stage-specific molecular mechanisms regulating drought stress responses in maize. Based on the transcriptome analysis, a total of 3451 differentially expressed genes (DEGs) were identified from the four experimental comparisons, with 2403, 650, 397 and 313 DEGS observed at the V12, VT, R1, and R4 stages, respectively. The expression changes in these genes effected corresponding metabolic pathway responses related to drought tolerance in maize. Subsequently, 3451 DEGs were divided into 12 modules by weighted gene co-expression network analysis (WGCNA), comprising 277 hub genes covering drought-responsive genes involved in water-deficit stress and developmental signaling crosstalk. Interestingly, the co-expressed genes that clustered into similar modules exhibited diverse expression tendencies and got annotated to different GO terms at different stages. MapMan analysis revealed that DEGs related to stress signal transduction, detoxification, transcription factor regulation, hormone signaling and secondary metabolites biosynthesis were universal across the four growth stages. However, the DEGs associated with photosynthesis and amino acid metabolism; protein degradation; transport; and RNA transcriptional regulation were uniquely enriched at the V12, VT, R2, and R4 stages, respectively. Conclusions Our results affirmed that maize drought stress adaptation is a whole-plant response as well as a stage-specific response process. We hope that our findings will aid in clarifying the fundamental cultivar-specific and growth-stage-specific molecular mechanisms regulating drought stress responses in maize. In addition, the genes and metabolic pathways identified here can be valuable genetic resources or selection targets for developing new drought resistant maize cultivars.

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Section: Genes Related To Hormone Signaling Are Critical For Drought supporting

confidence: 90%

Global transcriptome and weighted gene co-expression network analyses of growth-stage-specific and hybrid-cultivar-specific drought stress responses in maize

Liu¹,

Zenda²,

Jin³

et al. 2020

Preprint

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“…Proteins were extracted from floral buds using the modified method described previously [81]. Flower buds (500 mg) were ground to a fine powder in liquid nitrogen.…”

Section: Protein Extraction and Digestionmentioning

confidence: 99%

An Integrative Analysis of Transcriptome, Proteome and Hormones Reveals Key Differentially Expressed Genes and Metabolic Pathways Involved in Flower Development in Loquat

Jing

Chen

et al. 2020

IJMS

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Flower development is a vital developmental process in the life cycle of woody perennials, especially fruit trees. Herein, we used transcriptomic, proteomic, and hormone analyses to investigate the key candidate genes/proteins in loquat (Eriobotrya japonica) at the stages of flower bud differentiation (FBD), floral bud elongation (FBE), and floral anthesis (FA). Comparative transcriptome analysis showed that differentially expressed genes (DEGs) were mainly enriched in metabolic pathways of hormone signal transduction and starch and sucrose metabolism. Importantly, the DEGs of hormone signal transduction were significantly involved in the signaling pathways of auxin, gibberellins (GAs), cytokinin, ethylene, abscisic acid (ABA), jasmonic acid, and salicylic acid. Meanwhile, key floral integrator genes FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) and floral meristem identity genes SQUAMOSA PROMOTER BINDING LIKE (SPL), LEAFY (LFY), APETALA1 (AP1), and AP2 were significantly upregulated at the FBD stage. However, key floral organ identity genes AGAMOUS (AG), AP3, and PISTILLATA (PI) were significantly upregulated at the stages of FBE and FA. Furthermore, transcription factors (TFs) such as bHLH (basic helix-loop-helix), NAC (no apical meristem (NAM), Arabidopsis transcription activation factor (ATAF1/2) and cup-shaped cotyledon (CUC2)), MYB_related (myeloblastosis_related), ERF (ethylene response factor), and C2H2 (cysteine-2/histidine-2) were also significantly differentially expressed. Accordingly, comparative proteomic analysis of differentially accumulated proteins (DAPs) and combined enrichment of DEGs and DAPs showed that starch and sucrose metabolism was also significantly enriched. Concentrations of GA3 and zeatin were high before the FA stage, but ABA concentration remained high at the FA stage. Our results provide abundant sequence resources for clarifying the underlying mechanisms of the flower development in loquat.

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“…To gain deeper insight into somatic embryogenesis in conifers, a proteomic approach has been used in Picea glauca (Lippert et al, 2005), Picea asperata (Jing et al, 2017), Larix x eurolepis (Teyssier et al, 2014), P. pinaster Ait. (Morel et al, 2014a) and Araucaria angustifolia (dos Santos et al, 2016; Fraga et al, 2016a).…”

Section: Somatic Embryogenesis In Pinus Nigra Arnmentioning

confidence: 99%

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

et al. 2019

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Somatic embryogenesis was achieved in the conifers Pinus nigra Arn. and in the hybrids Abies alba ×A. cephalonica and Abies alba ×A. numidica. For initiation of embryogenic tissue in P. nigra, immature zygotic embryos enclosed in megagametophytes were used. The initiated embryogenic cultures were maintained and proliferated on solid culture medium DCR supplemented with 9 μM 2,4-D and 2.2 μM BA. Microscopic investigations revealed the presence of bipolar early somatic embryos in proliferating tissue. Suspension cultures have also been established by resuspending the embryogenic tissue in liquid culture medium. Experimentation with abscisic acid concentration resulted in successful somatic embryo maturation. Besides abscisic acid, the carbohydrate content or higher concentration of gelling agent in maturation medium were also important requirements for somatic embryo maturation. Germination of cotyledonary somatic embryos occurred on hormone-free medium and terminated in somatic seedlings regeneration. The regenerated somatic seedlings were transferred to soil and were capable of successful development. For initiation of embryogenic tissue in Abies hybrids juvenile explants as immature or mature zygotic embryos as well as cotyledons were used and 4.4 μM BA as sole plant growth regulator was sufficient. Medium of the same composition was also suitable for their long-term maintenance. Maturation of somatic embryos was achieved on solid DCR medium supplemented with 38 μM abscisic acid, polyethylene glycol (0, 5, 7.5, and 10% PEG-4000) and different carbohydrates such as maltose, sucrose and glucose (each 3%). PEG-4000 stimulated somatic embryo development depending on the carbohydrate source used. Cotyledonary somatic embryos germinated after desiccation treatment and the regenerated somatic seedlings were transferred to soil. Cryopreservation of embryogenic tissue could be an alternative method for long-term maintenance. For cryopreservation the slow-freezing method was used with success. Tissue regeneration in the post thaw period was relatively high and the regenerated tissue produced mature somatic embryos and subsequent plantlets. The embryogenic tissue was also used in experiments focused on genetic transformation either by biolistic (P. nigra) or Agrobacterium-mediated (Abies hybrids) methods. A proteomic study was performed to gain a deeper insight into the early stages of P. nigra somatic embryogenesis.

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Proteomic analysis of stress‐related proteins and metabolic pathways in Picea asperata somatic embryos during partial desiccation

Cited by 38 publications

References 56 publications

Global transcriptome and weighted gene co-expression network analyses of growth-stage-specific and hybrid-cultivar-specific drought stress responses in maize

Global transcriptome and weighted gene co-expression network analyses of growth-stage-specific and hybrid-cultivar-specific drought stress responses in maize

An Integrative Analysis of Transcriptome, Proteome and Hormones Reveals Key Differentially Expressed Genes and Metabolic Pathways Involved in Flower Development in Loquat

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

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