Post-Cercarial Development of Schistosoma mansoni in the Rabbit and Hamster after Intraperitoneal and Percutaneous Infection

Yolles, Tamarath K.; Moore, Donald V.; Meleney, Henry E.

doi:10.2307/3273303

Cited by 48 publications

(17 citation statements)

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“…BrieXy, after fasting for 24 h, mice were anesthetized using a mix of medetomidine (0.5 mg kg ¡1 ; Domitor; PWzer, New York, NY, USA) and ketamine (50 mg kg ¡1 ; Anesketin; Eurovet, Bladel, The Netherlands) dissolved in a physiological solution and administered intraperitoneally (Yolles et al 1949). After having been shaved and disinfected, the lower abdomen was incised and the ileum was exteriorized.…”

Section: Animalsmentioning

confidence: 99%

“…To prepare the S. mansoni-infected group, mice were infected with S. mansoni according to the method of Yolles et al (1949). BrieXy, the mice were anesthetized with an intraperitoneal injection of sodium pentobarbital (60 mg kg ¡1 ; Nembutal; SanoW, Brussels, Belgium), and 1 ml of sterile water containing 130 freshly shed cercariae of a Puerto Rico strain of S. mansoni was intraperitoneally injected.…”

Section: Animalsmentioning

confidence: 99%

“…(1) Schistosoma mansoniinduced intestinal schistosomiasis and (2) trinitrobenzene sulphonic acid (TNBS)-induced ileitis. Intestinal schistosomiasis is a parasitic helminth disease, which at the acute stage involves a granulomatous immunopathology with a Th2-type inXammatory response directed against parasite eggs that lodge in the liver and intestine (Vella and Pearce 1992;Yolles et al 1949). TNBS-induced ileitis at the acute stage is characterized by acute mucosal damage and a localized Th1/Th17-type inXammatory reaction in response to the haptenating agent (Elson et al 1996;Pontell et al 2009;Ruyssers et al 2009).…”

Section: Introductionmentioning

confidence: 98%

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The effect of inflammation on the expression and distribution of the MAS-related gene receptors MrgE and MrgF in the murine ileum

Avula

Buckinx

Alpaerts

et al. 2011

Histochem Cell Biol

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The MAS-related gene (Mrg) receptor MrgE has been suggested to be expressed at all tissue levels involved in pain sensation and to influence the expression of another Mrg receptor, MrgF. Given the knowledge on the role of the enteric nervous system (ENS) in sensation, and the plasticity of enteric neurons during intestinal inflammation, it can be hypothesized that MrgE is expressed in enteric neurons, and that MrgE and MrgF change expression in intestinal inflammatory conditions. Therefore, we aimed to reveal the expression details of MrgE and MrgF in the murine ileum in normal and inflamed conditions. Using reverse transcriptase-PCR, quantitative-PCR and immunohistochemistry, we compared the ileum of non-inflamed control mice with that of two models of intestinal inflammation, i.e. intestinal schistosomiasis and chemically induced ileitis. MrgE and MrgF mRNAs were detected in control and inflamed conditions. MrgE and MrgF mRNAs showed a trend towards downregulation during intestinal schistosomiasis and a significant reduction during ileitis. MrgE and MrgF receptors were expressed in distinct enteric neuronal subpopulations, such as the sensory, secretomotor and vasodilator neurons, and in nerve fibres in the tunica muscularis and lamina propria of control and inflamed ileum. Only a minor proportion of enteric neurons co-expressed MrgE and MrgF. The number of enteric neurons expressing MrgE and MrgF receptors was significantly reduced during intestinal schistosomiasis and ileitis. This is the first report on the expression of MrgE and MrgF in the ENS in (patho)physiological conditions. The expression of MrgE and MrgF in enteric neurons was negatively affected by inflammation.

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Section: Animalsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

The effect of inflammation on the expression and distribution of the MAS-related gene receptors MrgE and MrgF in the murine ileum

Avula

Buckinx

Alpaerts

et al. 2011

Histochem Cell Biol

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“…Heterozygous male CX3CR1 +/GFP C57BL/6 mice, 8 weeks old, were intraperitoneally (i.p.) infected with a Puerto Rican strain of S. mansoni following an adapted procedure of Yolles et al . Animals were sacrificed 8 weeks post infection (wpi).…”

Section: Methodsmentioning

confidence: 99%

Effect of schistosomiasis on CX3CR1‐expressing mononuclear phagocytes in the ileum and mesenteric lymph nodes of the mouse

Alpaerts

Buckinx

Cools

et al. 2015

Neurogastroenterology Motil

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The significant upregulation of CD11c(+) CX3CR1(+) F4/80(-) DCs during intestinal schistosomiasis and the restriction of phagocytosis of parasitic antigens to CX3CR1-expresssing MNP indicate a crucial role for this immune cell niche in response to trematodiasis. These findings add insight into the functional specialization of LP immune cells and add to the understanding of cellular mechanisms behind helminth-based therapies.

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“…Mice were infected with S. mansoni according to the method of Yolles et al [28]. Briefly, the mice were anesthetized with an intraperitoneal injection of sodium pentobarbital (60 mg kg -1 ; NEMBUTAL; Sanofi, Brussels, Belgium) followed by an intraperitoneal injection of 1 ml of sterile water containing 130 freshly shed cercariae of a Puerto Rico strain of S. mansoni .…”

Section: Methodsmentioning

confidence: 99%

Whole-genome microarray analysis and functional characterization reveal distinct gene expression profiles and patterns in two mouse models of ileal inflammation

et al. 2012

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BackgroundAlthough a number of intestinal inflammatory conditions pertain to the ileum, whole-genome gene expression analyses in animal models of ileal inflammation are lacking to date. Therefore, we aimed to identify and characterize alterations in gene expression in the acutely inflamed ileum of two murine models of intestinal inflammation, namely intestinal schistosomiasis and TNBS-induced ileitis, compared to healthy controls. To this end, we used whole-genome microarrays, followed by bioinformatics analyses to detect over-represented Kyoto Encyclopedia of Genes and Genomes pathways and Gene Ontology categories.ResultsFollowing screening of almost all known mouse genes and transcripts represented on the array, intestinal schistosomiasis and TNBS-induced ileitis yielded 207 and 1417 differentially expressed genes, respectively, with only 30 overlapping concordantly changed genes. Functional category groups consisting of complement and coagulation cascades, extracellular matrix (ECM)-receptor interaction, Fc epsilon receptor I signaling pathways and protein activation cascade, cell adhesion categories were over-represented in the differential gene list of intestinal schistosomiasis. Antigen processing and presentation, cell adhesion molecules, ABC transporters, Toll-like receptor signaling pathways and response to chemical stimulus categories were over-represented in the differential gene list of TNBS-induced ileitis. Although cytokine-cytokine receptor interaction, intestinal immune network for IgA production, focal adhesion pathways and immune, inflammatory and defense response categories were over-represented in the differential gene lists of both inflammation models, the vast majority of the associated genes and changes were unique to each model.ConclusionsThis study characterized two models of ileal inflammation at a whole-genome level and outlined distinct gene expression profiles and patterns in the two models. The results indicate that intestinal schistosomiasis involves Th2 responses, complement activation, protein activation and enhanced ECM turnover, while TNBS-induced ileitis involves Th17 responses, defective antigen processing and presentation and altered Toll-like receptor-mediated responses. Signs of an impaired epithelial barrier are apparent in both inflammation models. Furthermore, the comprehensive differential gene list and functional groups provided by this study constitute an interesting starting point to explore new targets and extended functional networks dealing with small bowel inflammation.

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Post-Cercarial Development of Schistosoma mansoni in the Rabbit and Hamster after Intraperitoneal and Percutaneous Infection

Cited by 48 publications

References 5 publications

The effect of inflammation on the expression and distribution of the MAS-related gene receptors MrgE and MrgF in the murine ileum

The effect of inflammation on the expression and distribution of the MAS-related gene receptors MrgE and MrgF in the murine ileum

Effect of schistosomiasis on CX3CR1‐expressing mononuclear phagocytes in the ileum and mesenteric lymph nodes of the mouse

Whole-genome microarray analysis and functional characterization reveal distinct gene expression profiles and patterns in two mouse models of ileal inflammation

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