Effect of schistosomiasis on CX3CR1‐expressing mononuclear phagocytes in the ileum and mesenteric lymph nodes of the mouse

Abstract

The significant upregulation of CD11c(+) CX3CR1(+) F4/80(-) DCs during intestinal schistosomiasis and the restriction of phagocytosis of parasitic antigens to CX3CR1-expresssing MNP indicate a crucial role for this immune cell niche in response to trematodiasis. These findings add insight into the functional specialization of LP immune cells and add to the understanding of cellular mechanisms behind helminth-based therapies.

Identifying Therapeutic Targets for Sepsis Research: A Characterization Study of the Inflammatory Players in the Cecal Ligation and Puncture Model

“…Flow cytometric identification of different immune cells was performed as described elsewhere [ 37 , 40 , 41 ]. In short, spleens and MLN collected in full RPMI medium were manually dissociated applying a 10 ml syringe plunger over a 40 μ M cell strainer (Falcon®, BD) and rinsed with RPMI followed by 5 min centrifugation at 4°C, 1500 rpm.…”

“…In short, for all samples, cell aggregates were excluded based upon the forward scatter area versus forward scatter height plot (FSC-A/FSC-H). Then, leukocytes were gated based upon their FSC-H and side scatter H (SSC-H) characteristics in order to exclude debris [ 40 , 42 , 48 ]. Finally, cell populations were defined based upon their expression of several cell surface markers (Supplementary Table 4 ) and cell subsets were expressed as a percentage of the total leukocyte count in each studied tissue.…”

Open multi-organ communication device for easy interrogation of tissue slices

“…For all experiments that involved living tissue slices, 300 μm slices of the PP were placed in culture well #1 and 300 μm slices of the MLN were placed in culture well #2. PP's were always placed in the first well to mimic the physiological communication between the PP and MLN in vivo (lymphatic flow travels from the PP to the MLN 9,41,42 ). Media was perfused into the inlet of culture well #1 through the device and pulled from the outlet of culture well #2 into a waste container via an external peristaltic pump.…”