Open multi-organ communication device for easy interrogation of tissue slices

Delong, Lauren M.; Ross, Ashley E.

doi:10.1039/d3lc00115f

Cited by 4 publications

(2 citation statements)

References 51 publications

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“…200 Further, Delong and Ross (2023) developed a multi-organ system to allow for communication between ex vivo Peyer's patches and mesenteric lymph node slices. 201 The advances in immune models will hopefully aid in developing more advanced gut–immune models in future, allowing us to discover more about the intestinal immune system and its role in the development and progression of various diseases.…”

Section: Gut–immune Lab-on-chip Technologies For Metabolic Disease Re...mentioning

confidence: 99%

Lab-on-chip technologies for exploring the gut–immune axis in metabolic disease

Wheeler,

Stoeger,

Owens

2024

Lab Chip

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Section: Gut–immune Lab-on-chip Technologies For Metabolic Disease Re...mentioning

confidence: 99%

Lab-on-chip technologies for exploring the gut–immune axis in metabolic disease

Wheeler,

Stoeger,

Owens

2024

Lab Chip

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“…23 Microscale and organoid models of the LN are still in early stages even in isolation, 20,[24][25][26][27][28][29][30] let alone in connection with other organs. [31][32][33] The model with the longest history is ex vivo LN slice culture, which have been used to model the response to infection and vaccination in humans and animals for thirty years. [34][35][36][37][38] Here, we developed a user-friendly, self-contained system for multi-tissue culture under continuous recirculating fluid flow, and applied it to model the communication between the lymph node and upstream drainage sites.…”

Section: Introductionmentioning

confidence: 99%

A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node

Cook,

Ball,

Mohammad

et al. 2024

Preprint

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Multi-organ-on-chip systems (MOOCs) have the potential to mimic communication between organ systems and reveal mechanisms of health and disease. However, many existing MOOCs are challenging for non-experts to implement, due to complex tubing, electronics, or pump mechanisms. In addition, few MOOCs have incorporated immune organs such as the lymph node (LN), limiting their applicability to critical events such as vaccination. Here we developed a 3D-printed, user-friendly device and companion tubing-free impeller pump to co-culture two or more tissue samples, including a LN, under a recirculating common media. Native tissue structure and immune function were incorporated by maintaining slices of murine LN tissue ex vivo in 3D- printed mesh supports for at least 24 hr. In a two-compartment model of a LN and an upstream injection site, vaccination of the multi-tissue chip was similar to in vivo vaccination in terms of locations of antigen accumulation and acute changes in activation markers and gene expression in the LN. We anticipate that in the future, this flexible platform will enable models of multi-organ immune responses throughout the body.

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Recent Advances in Gut‐ and Gut–Organ‐Axis‐on‐a‐Chip Models

Kim,

Sung

2024

Adv Healthcare Materials

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The human gut extracts nutrients from the diet while forming the largest barrier against the outer environment. In addition, the gut actively maintains homeostasis through intricate interactions with the gut microbes, the immune system, the enteric nervous system, and other organs. These interactions influence digestive health and, furthermore, play crucial roles in systemic health and disease. Given its primary role in absorbing and metabolizing orally administered drugs, there is significant interest in the development of preclinical in vitro model systems that can accurately emulate the intestine in vivo. A gut‐on‐a‐chip system holds great potential as a testing and screening platform because of its ability to emulate the physiological aspects of in vivo tissues and expandability to incorporate and combine with other organs. This review aims to identify the key physiological features of the human gut that need to be incorporated to build more accurate preclinical models and highlights the recent progress in gut‐on‐a‐chip systems and competing technologies toward building more physiologically relevant preclinical model systems. Furthermore, various efforts to construct multi‐organ systems with the gut, called gut‐organ‐axis‐on‐a‐chip models, are discussed. In vitro gut models with physiological relevance can provide valuable platforms for bridging the gap between preclinical and clinical studies.This article is protected by copyright. All rights reserved

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Open multi-organ communication device for easy interrogation of tissue slices

Abstract: Here, we have developed an open multi-organ communication device that facilitates cellular and molecular communication between ex-vivo organ slices. Measuring communication between organs is vital for understanding the mechanisms of...

Cited by 4 publications

References 51 publications

Lab-on-chip technologies for exploring the gut–immune axis in metabolic disease

Lab-on-chip technologies for exploring the gut–immune axis in metabolic disease

A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node

Recent Advances in Gut‐ and Gut–Organ‐Axis‐on‐a‐Chip Models

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