Possible role of mucosal mast cells in the recovery process of colitis induced by dextran sulfate sodium in rats

Iba, Yoshinori; Sugimoto, Yukihiko; Kamei, Chiaki; Masukawa, Tohru

doi:10.1016/s1567-5769(02)00299-0

Cited by 40 publications

(37 citation statements)

References 21 publications

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“…Most of these histological changes have been observed by others in several DSS colitis rat models (Gaudio et al, 1999;Iba et al, 2003;Herias et al, 2005). Peculiar to our study, we noted that the severity of these lesions increased with time so that the most severe lesions were observed in Cycle 3, less severe in Cycle 2, and the least severe in Cycle 1.…”

Section: Discussionsupporting

confidence: 86%

“…This harmonizes with other experimental setups in which chronic colitis signs were observed several days after replacement of DSS with normal drinking water. According to other researchers, these signs can be observed as long as 10 d after replacement of DSS with drinking water (Kullmann et al, 2001;Iba et al, 2003). Since there were only 7 d of treatment with drinking water between cycles in our setup, the observed clinical signs on the beginning of Cycles 2 and 3 are tied to the preceding cycle of DSS treatment.…”

Section: Discussionmentioning

confidence: 59%

“…In several rat models of DSS colitis, diarrhea, loose stools, hemoccult positive stools, gross bleeding, and loss of body weight are common signs observed (Gaudio et al, 1999;Kullmann et al, 2001;Vetuschi et al, 2002;Iba et al, 2003). They can occur as early as Day 2 in acute colitis rat models and as Day 4 in chronic models (Kullmann et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Polymorphonuclear neutrophil infiltration is regarded as the primary and central lesion of colitis that is usually followed by loss of the epithelium, loss of goblet cells and crypt damage (Iba et al, 2003). Stemming on the evidence that SA decreases the neutrophil infiltration in the colon mucosa of colitis models and the fact that infiltration of neutrophils to this mucosa is central to colitis, it can be argued that SA may inhibit development of other lesions of colitis and subsequently prevent colitis.…”

Section: Inflammatory Bowel Diseases (Ibd) That Includementioning

confidence: 99%

“…After deparaffinizing the thin tissue sections on glass slides, they were stained with hematoxylin and eosin (H & E). For histological evaluation of the tissue damage, mucosal integrity was assessed in a blinded manner and quantitated by two independent pathologists using a slightly modified method by Iba et al(2003). The scoring was on a 0~3 scale as shown in Table 2.…”

Section: Evaluation Of Histopathological Findingsmentioning

confidence: 99%

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Sodium arsenite reduces severity of dextran sulfate sodium-induced ulcerative colitis in rats

Malago

Nondoli²

2008

J. Zhejiang Univ. Sci. B

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Abstract:The histopathological features and the associated clinical findings of ulcerative colitis (UC) are due to persistent inflammatory response in the colon mucosa. Interventions that suppress this response benefit UC patients. We tested whether sodium arsenite (SA) benefits rats with dextran sulfate sodium (DSS)-colitis. The DSS-colitis was induced by 5% DSS in drinking water. SA (10 mg/kg; intraperitoneally) was given 8 h before DSS treatment and then every 48 h for 3 cycles of 7, 14 or 21 d. At the end of each cycle rats were sacrificed and colon sections processed for histological examination. DSS induced diarrhea, loose stools, hemoccult positive stools, gross bleeding, loss of body weight, loss of epithelium, crypt damage, depletion of goblet cells and infiltration of inflammatory cells. The severity of these changes increased in the order of Cycles 1, 2 and 3. Treatment of rats with SA significantly reduced this severity and improved the weight gain.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Inflammatory Bowel Diseases (Ibd) That Includementioning

confidence: 99%

Section: Evaluation Of Histopathological Findingsmentioning

confidence: 99%

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Sodium arsenite reduces severity of dextran sulfate sodium-induced ulcerative colitis in rats

Malago

Nondoli²

2008

J. Zhejiang Univ. Sci. B

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The role of galanin in the differentiation of mucosal mast cells in mice

et al. 2019

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Mast cells are generally classified into two phenotypically distinct populations: mucosal‐type mast cells (MMCs) and connective tissue‐type mast cells (CTMCs). However, the molecular basis determining the different characteristics of the mast cell subclasses still remains unclear. Unfortunately, the number of mast cells that can be obtained from tissues is limited, which makes it difficult to study the function of each mast cell subclass. Here, we report the generation and characterization of MMCs and CTMCs derived from mouse BM mast cells (BMMCs). We found that the expression of galanin receptor 3 was elevated in MMCs when compared to the expression in CTMCs. Moreover, intraperitoneal injection of a galanin antagonist reduced MMCs and inhibited the inflammation of dextran sodium sulfate‐induced colitis in mice. Therefore, these results suggest that galanin promotes MMC differentiation in vivo, and provide important insights into the molecular mechanisms underlying the differentiation of mast cell subclasses.

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