Possible role of glutathione in mitochondrial apoptosis of human oral squamous cell carcinoma caused by inorganic selenium compounds

Takahashi, Masato; Sato, Tomonori; Shinohara, Fumiaki; Echigo, Seishi; Rikiishi, Hidemi

doi:10.3892/ijo.27.2.489

Cited by 20 publications

(41 citation statements)

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“…Morales et al (2005) recently confirmed these results, by showing that intracellular glutathione levels determine cell sensitivity to drug-induced apoptosis. It has also been demonstrated that intracellular GSH depletion of human oral squamous cell carcinoma by inorganic selenium compounds may cause caspase-9-mediated apoptosis (Takahashi et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Prognostic impact of multidrug resistance gene expression on the management of breast cancer in the context of adjuvant therapy based on a series of 171 patients

et al. 2006

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Study of the prognostic impact of multidrug resistance gene expression in the management of breast cancer in the context of adjuvant therapy. This study involved 171 patients treated by surgery, adjuvant chemotherapy7radiotherapy7hormonal therapy (mean follow-up: 55 months). We studied the expression of multidrug resistance gene 1 (MDR1), multidrug resistance-associated protein (MRP1), and glutathione-S-transferase P1 (GSTP1) using a standardised, semiquantitative rt -PCR method performed on frozen samples of breast cancer tissue. Patients were classified as presenting low or high levels of expression of these three genes. rt-PCR values were correlated with T stage, N stage, Scarff -Bloom -Richardson (SBR) grade, age and hormonal status. The impact of gene expression levels on 5-year disease-free survival (DFS) and overall survival (OS) was studied by univariate and multivariate Cox analysis. No statistically significant correlation was demonstrated between MDR1, MRP1 and GSTP1 expressions. On univariate analysis, DFS was significantly decreased in a context of low GSTP1 expression (P ¼ 0.0005) and high SBR grade (P ¼ 0.003), size X5 cm (P ¼ 0.038), high T stage (P ¼ 0.013), presence of intravascular embolus (P ¼ 0.034), and 43 N þ (P ¼ 0.05). On multivariate analysis, GSTP1 expression and the presence of ER remained independent prognostic factors for DFS. GSTP1 expression did not affect OS. The levels of MDR1 and MRP1 expression had no significant influence on DFS or OS. GSTP1 expression can be considered to be an independent prognostic factor for DFS in patients receiving adjuvant chemotherapy for breast cancer.

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Section: Discussionmentioning

confidence: 99%

Prognostic impact of multidrug resistance gene expression on the management of breast cancer in the context of adjuvant therapy based on a series of 171 patients

et al. 2006

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“…Analysis was then carried out on a FACScalibur. For the positive control, cells were activated with 50 μM SeO 2 (Sigma-Aldrich) (15).…”

Section: Assessment Of Mitochondrial Membrane Potential (δAem)mentioning

confidence: 99%

Sequence-dependent interaction between cisplatin and histone deacetylase inhibitors in human oral squamous cell carcinoma cells

Sato

Suzuki²,

Sato³

et al. 2006

Int J Oncol

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Abstract. Chemotherapeutic treatment with combinations of drugs is front-line therapy for many types of cancer. Combining drugs that target different signaling pathways often lessens adverse side-effects while increasing the efficacy of treatment and reducing patient morbidity. Histone deacetylase (HDAC) inhibitors represent a novel class of anti-neoplastic agents that act by promoting acetylation of core histones, leading in turn to the uncoiling of chromatin and activation of a variety of genes implicated in the regulation of cell survival, proliferation, differentiation, and apoptosis. A defined scheduling protocol is described by which HDAC inhibitors facilitate the cytotoxic effectiveness of cisplatin (CDDP) in the killing of carcinoma cells. An oral squamous cell carcinoma cell line (HSC-3) was treated with sodium butyrate (NaB), suberoylanilide hydroxamic acid (SAHA) or MS-275 on the day of, the day before, or the day after addition of CDDP. The IC 50 (48-h assay) value of 3.48 μg/ml CDDP could be lowered to 0.41 μg/ml CDDP when concurrently combined with an HDAC inhibitor (MS-275). The percentage of apoptosis by treatment with CDDP for 24 h, followed by NaB for an additional 24 h without washing was significantly greater than that observed in the reverse order. Depending on the time of addition of HDAC inhibitors, CDDP-treated cells displayed varying degrees of apoptotic responses, indicating the critical nature of timing in the use of HDAC inhibitors. Interestingly, experiments suggested that cells arrested at the G 1 /S checkpoint by CDDP were more sensitive to subsequent treatment with an HDAC inhibitor. Moreover, these events were associated with an enhancement of reactive oxygen species (ROS) generation and caspase-3 activation by HDAC inhibitors. They raise the possibility that combining these agents may represent a novel anti-neoplastic strategy.

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“…In a random, double-blind cancer prevention trial, the incidence of prostate cancer was reduced by 63% compared to the placebo group upon selenium supplementation of 200 μg/d as selenium-enriched yeast. Similar studies also showed a noticeable decline in lung and colorectal cancers, as well as in total cancer mortality rates [105,106]. In a separate trial, patients with uterine cervical carcinoma were found to have low glutathione peroxidases and selenium levels [106].…”

Section: Selenium Deficiencymentioning

confidence: 74%

“…Similar studies also showed a noticeable decline in lung and colorectal cancers, as well as in total cancer mortality rates [105,106]. In a separate trial, patients with uterine cervical carcinoma were found to have low glutathione peroxidases and selenium levels [106].…”

Section: Selenium Deficiencymentioning

confidence: 74%

“…In the case of inorganic selenium compounds, sodium selenite is the compound of choice for both antioxidant and anticancer studies. While the mechanism for its antioxidant activity is unclear, it has been proposed that the ability of selenocysteine containing enzymes such as GPx and thioredoxin reductases to prevent radical formation as a possible mechanism [106].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Green Synthesis of Selenium Nanoparticles from Broccoli, Characterization, Application and Toxicity

Kapur¹,

Soni²,

Kohli³

2017

Adv Tech Biol Med

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Selenium in broccoliBroccoli belongs to the Brassicaceae family, originated in the Mediterranean regions and distributed in Europe and in the United States. Broccoli is rich in micronutrients such as carotene, vitamin C and folic acid-fibers, phytochemicals such as glucosinolates (GLs) and *Corresponding author: Kanchan Kohli, PhD, Associate Professor, Department of Pharmaceutics, Jamia Hamdard, Hamdard University, New Delhi 110062, India, Tel: +911126059688; E-mail: drkanchankohli@gmail.com AbstractPlant-based diets and phytochemicals present in plants can be used to decrease the risk of cancer. This article deals with Brassica species and broccoli in particular which are associated with reduced risk of several important cancers. These plants mainly contain selenium (Se) covalently bound in a number of different chemical forms which is mainly responsible for its biological activity. Further, several enzymes contain selenium in the form of the unusual selenocysteine amino acid which act on free radicals. Broccoli has the ability to accumulate Se many-fold beyond the concentration of Se present in the soil. Reflecting on the above it can be suggested that development of methods to increase the natural accumulation of Se in broccoli may greatly enhance its health-promoting properties. The present article details about green synthesis of selenium nanoparticles and their methods of characterizations. Green synthesis is a biological procedure which can be achieved can be achieved by using plant extracts. This method is capable of producing SeNPs in a size range of about 50-150 nm, under ambient conditions. It was found that SeNPs are able to inhibit the cell growth by dose-dependent manner. In addition, combination of SeNPs and doxorubicin shows better anticancer effect than individual treatments. The present review also brings to light about the causes and implications of toxic effects posed by Se nanoparticles as well as applications of Se nanoparticles in medicine.Green Synthesis of Selenium Nanoparticles from Broccoli, Characterization, Application and Toxicity Citation: Kapur M, Soni K, Kohli K (2017) Green Synthesis of Selenium Nanoparticles from Broccoli, Characterization, Application and Toxicity. Adv Tech Biol Med 5: 198. doi: 10.4172/2379-1764.1000198 Page 2 of 7 ManaviVolume Se Nanoparticles Characterization [42]An initial characterization of the test substance is imperative before any toxicity screening is commenced. A more extensive and complete characterization, including size distribution, shape, surface area, surface chemistry, crystallinity, porosity, agglomeration state, surface charge, solubility, etc., is recommended for nanomaterials in order to determine the correct correlation between their physicochemical properties and the biological effects they elicit [43][44][45][46].

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Possible role of glutathione in mitochondrial apoptosis of human oral squamous cell carcinoma caused by inorganic selenium compounds

Cited by 20 publications

References 0 publications

Prognostic impact of multidrug resistance gene expression on the management of breast cancer in the context of adjuvant therapy based on a series of 171 patients

Prognostic impact of multidrug resistance gene expression on the management of breast cancer in the context of adjuvant therapy based on a series of 171 patients

Sequence-dependent interaction between cisplatin and histone deacetylase inhibitors in human oral squamous cell carcinoma cells

Green Synthesis of Selenium Nanoparticles from Broccoli, Characterization, Application and Toxicity

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