Possible juvenile-related proteins in olive tree tissues

Differential Protein Expression in Olive Tissues Due to Salt Treatments

“…Leaves and stems of each treatment and clone were grounded separately in liquid nitrogen and stored at -80 °C until use. Protein extraction was performed as described previously (García et al, 2000) and protein concentration of the extracts according to Bradford (1976).…”

“…Differential protein expression was reported for juvenile and mature olive tissues (García et al, 2000;García et al, 2002). In relation to stress resistance, Bartolini et al (1994) found different protein patterns between tolerant and non tolerant clones of cv.…”

Nutritional and Proteomic Profiles in Developing Olive Inflorescence

“…A modified protocol based on those suggested by Garcia et al (2000) Süle et al (2004), and Wang et al (2003and Wang et al ( , 2004 for olive was followed for protein extraction and purification. Proteins were extracted using a 10fold volume/weight buffer (50 mM sodium borate, 50 mM ascorbic acid pH 9.0, 1% β-mercaptoethanol, 1% soluble PVP, 1% insoluble PVP, 10 mM PMSF) under continuous vortexing for 60 min at 4 o C. The homogenate was centrifuged at 4 o C for 30 min at 35,000 g and the pellet was discarded.…”

“…The pellets for SDS-PAGE analysis (about 50 μg) were solubilised in sample buffer 62,5 mM Tris-HCl, pH 6.8, 2% SDS, 5% β-mercaptoethanol, 10% glycerol and traces of bromophenol blue (Garcia et al 2000) enhanced with 8.0 M urea, and denatured by heating in boiling water for 5 min. Then cooled to room temperature and centrifuged for 5 min at 14,000g.…”

“…However, little effort have been done on olive proteomic, probably because of the difficulty on sample handling, due to the presence of many phenolic compounds (Garcia et al 2000, Wang et al, 2003, Wang et al, 2004, Wang et al, 2010.…”